Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse
[Objective:] To reconstruct the structure of mouse tooth germ in the early stage of development, and to compare the morphological differences between the rudimentary bud 2 (R2) in the diastema region and the first molar (M1) in the molar region. [Methods:] After being fixed, the head specimens of E1...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-10-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.05.002 |
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| _version_ | 1849225125417713664 |
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| author | ZHANG Han SUN Yao |
| author_facet | ZHANG Han SUN Yao |
| author_sort | ZHANG Han |
| collection | DOAJ |
| description | [Objective:] To reconstruct the structure of mouse tooth germ in the early stage of development, and to compare the morphological differences between the rudimentary bud 2 (R2) in the diastema region and the first molar (M1) in the molar region. [Methods:] After being fixed, the head specimens of E12.5 and E13.5 mouse embryos were embedded in paraffin, serially sectioned in the frontal plane, and stained with hematoxylin and eosin (HE). All images were processed with Photoshop CC 2018 software and Fiji software, then imported into Mimics Research 19.0 software for three-dimensional reconstruction and analysis. [Results:] It was observed in the reconstructed three-dimensional model that the epithelium of the tooth germ in the diastema forms a bud shape, but there was no obvious condensation of mesenchyme cells below it. After assigning virtual CT values to the R2 and M1, semi-quantitative measurements of the reconstructed images revealed that the mesenchymal cells coalescing around the bud-like epithelium of R2 were less dense and had a smaller condensation area. This phenomenon may be related to less cell proliferation in the diastema region. [Conclusion:] Three-dimensional reconstructions based on histological sections revealed a loss of the ability to form the condensational mesenchymal cell cluster in the diastema region of mice during early tooth development. |
| format | Article |
| id | doaj-art-be83b4b288f94a3cbf7d74b71a9181fe |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-10-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-be83b4b288f94a3cbf7d74b71a9181fe2025-08-25T06:10:21ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-10-0133528529110.12439/kqhm.1005-4979.2023.05.002Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouseZHANG Han0SUN Yao1Department of Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, ChinaDepartment of Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China[Objective:] To reconstruct the structure of mouse tooth germ in the early stage of development, and to compare the morphological differences between the rudimentary bud 2 (R2) in the diastema region and the first molar (M1) in the molar region. [Methods:] After being fixed, the head specimens of E12.5 and E13.5 mouse embryos were embedded in paraffin, serially sectioned in the frontal plane, and stained with hematoxylin and eosin (HE). All images were processed with Photoshop CC 2018 software and Fiji software, then imported into Mimics Research 19.0 software for three-dimensional reconstruction and analysis. [Results:] It was observed in the reconstructed three-dimensional model that the epithelium of the tooth germ in the diastema forms a bud shape, but there was no obvious condensation of mesenchyme cells below it. After assigning virtual CT values to the R2 and M1, semi-quantitative measurements of the reconstructed images revealed that the mesenchymal cells coalescing around the bud-like epithelium of R2 were less dense and had a smaller condensation area. This phenomenon may be related to less cell proliferation in the diastema region. [Conclusion:] Three-dimensional reconstructions based on histological sections revealed a loss of the ability to form the condensational mesenchymal cell cluster in the diastema region of mice during early tooth development.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.05.002three-dimensional reconstructiontooth developmenttooth germdiastemamesenchyme |
| spellingShingle | ZHANG Han SUN Yao Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse Kouqiang hemian waike zazhi three-dimensional reconstruction tooth development tooth germ diastema mesenchyme |
| title | Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse |
| title_full | Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse |
| title_fullStr | Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse |
| title_full_unstemmed | Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse |
| title_short | Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse |
| title_sort | three dimensional reconstruction of the early development of tooth germ an experimental study in the mouse |
| topic | three-dimensional reconstruction tooth development tooth germ diastema mesenchyme |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.05.002 |
| work_keys_str_mv | AT zhanghan threedimensionalreconstructionoftheearlydevelopmentoftoothgermanexperimentalstudyinthemouse AT sunyao threedimensionalreconstructionoftheearlydevelopmentoftoothgermanexperimentalstudyinthemouse |