Three-dimensional reconstruction of the early development of tooth germ: An experimental study in the mouse
[Objective:] To reconstruct the structure of mouse tooth germ in the early stage of development, and to compare the morphological differences between the rudimentary bud 2 (R2) in the diastema region and the first molar (M1) in the molar region. [Methods:] After being fixed, the head specimens of E1...
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| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-10-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.05.002 |
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| Summary: | [Objective:] To reconstruct the structure of mouse tooth germ in the early stage of development, and to compare the morphological differences between the rudimentary bud 2 (R2) in the diastema region and the first molar (M1) in the molar region. [Methods:] After being fixed, the head specimens of E12.5 and E13.5 mouse embryos were embedded in paraffin, serially sectioned in the frontal plane, and stained with hematoxylin and eosin (HE). All images were processed with Photoshop CC 2018 software and Fiji software, then imported into Mimics Research 19.0 software for three-dimensional reconstruction and analysis. [Results:] It was observed in the reconstructed three-dimensional model that the epithelium of the tooth germ in the diastema forms a bud shape, but there was no obvious condensation of mesenchyme cells below it. After assigning virtual CT values to the R2 and M1, semi-quantitative measurements of the reconstructed images revealed that the mesenchymal cells coalescing around the bud-like epithelium of R2 were less dense and had a smaller condensation area. This phenomenon may be related to less cell proliferation in the diastema region. [Conclusion:] Three-dimensional reconstructions based on histological sections revealed a loss of the ability to form the condensational mesenchymal cell cluster in the diastema region of mice during early tooth development. |
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| ISSN: | 1005-4979 |