Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses
Gingival and osseous augmentations are reported as hypertrophic or hyperplastic reactions to different factors including chronic traumatisms and surgeries such as free gingival graft (FGG) that induce an abnormal growth of both hard and soft tissues in genetically predisposed subjects. Since an imba...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Case Reports in Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2019/3231759 |
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| author | Luca Francetti Claudia Dellavia Stefano Corbella Nicolò Cavalli Claudia Moscheni Elena Canciani Nicoletta Gagliano |
| author_facet | Luca Francetti Claudia Dellavia Stefano Corbella Nicolò Cavalli Claudia Moscheni Elena Canciani Nicoletta Gagliano |
| author_sort | Luca Francetti |
| collection | DOAJ |
| description | Gingival and osseous augmentations are reported as hypertrophic or hyperplastic reactions to different factors including chronic traumatisms and surgeries such as free gingival graft (FGG) that induce an abnormal growth of both hard and soft tissues in genetically predisposed subjects. Since an imbalance in collagen turnover plays a key role in the development of gingival overgrowth leading to an accumulation of collagen in gingival connective tissue, in this study we described the histological and molecular features of three oral overgrowths obtained from a 34-year-old woman previously operated for FGG in order to evaluate a possible relationship between exostoses and overgrown tissue. Healthy and overgrown gingiva were analyzed by histological methods, and the expression of genes and proteins involved in collagen synthesis, maturation, and degradation was assessed in cultured fibroblasts obtained from gingival fragments at the molecular level. Our results show that general morphology and collagen content were similar in healthy and overgrown gingivae. However, fibroblasts obtained from the overgrown gingiva revealed an anabolic phenotype characterized by an increased collagen turnover and maturation. These findings indicate that an exostosis could act as a mechanical stimulus stretching the overlying connective tissue and triggering an anabolic phenotype of gingival fibroblasts and suggest to use minimally invasive surgical techniques to avoid traumatizing the periosteal tissues for the eradication of the exostosis with minimal relapses. |
| format | Article |
| id | doaj-art-be76a144d3124e04abab52a17ba2d07b |
| institution | OA Journals |
| issn | 2090-6447 2090-6455 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Case Reports in Dentistry |
| spelling | doaj-art-be76a144d3124e04abab52a17ba2d07b2025-08-20T02:18:35ZengWileyCase Reports in Dentistry2090-64472090-64552019-01-01201910.1155/2019/32317593231759Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral ExostosesLuca Francetti0Claudia Dellavia1Stefano Corbella2Nicolò Cavalli3Claudia Moscheni4Elena Canciani5Nicoletta Gagliano6Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical and Clinical Sciences “L. Sacco”, Università degli Studi di Milano, via G.B. Grassi 74, 20157 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical Sciences for Health, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, ItalyGingival and osseous augmentations are reported as hypertrophic or hyperplastic reactions to different factors including chronic traumatisms and surgeries such as free gingival graft (FGG) that induce an abnormal growth of both hard and soft tissues in genetically predisposed subjects. Since an imbalance in collagen turnover plays a key role in the development of gingival overgrowth leading to an accumulation of collagen in gingival connective tissue, in this study we described the histological and molecular features of three oral overgrowths obtained from a 34-year-old woman previously operated for FGG in order to evaluate a possible relationship between exostoses and overgrown tissue. Healthy and overgrown gingiva were analyzed by histological methods, and the expression of genes and proteins involved in collagen synthesis, maturation, and degradation was assessed in cultured fibroblasts obtained from gingival fragments at the molecular level. Our results show that general morphology and collagen content were similar in healthy and overgrown gingivae. However, fibroblasts obtained from the overgrown gingiva revealed an anabolic phenotype characterized by an increased collagen turnover and maturation. These findings indicate that an exostosis could act as a mechanical stimulus stretching the overlying connective tissue and triggering an anabolic phenotype of gingival fibroblasts and suggest to use minimally invasive surgical techniques to avoid traumatizing the periosteal tissues for the eradication of the exostosis with minimal relapses.http://dx.doi.org/10.1155/2019/3231759 |
| spellingShingle | Luca Francetti Claudia Dellavia Stefano Corbella Nicolò Cavalli Claudia Moscheni Elena Canciani Nicoletta Gagliano Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses Case Reports in Dentistry |
| title | Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses |
| title_full | Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses |
| title_fullStr | Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses |
| title_full_unstemmed | Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses |
| title_short | Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses |
| title_sort | morphological and molecular characterization of human gingival tissue overlying multiple oral exostoses |
| url | http://dx.doi.org/10.1155/2019/3231759 |
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