Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia
Objective. In 2023–2014, a significant increase in the incidence of pneumonia caused by M. pneumoniae has been reported in Russia and China. Despite being one of the primary causative agents of community-acquired lower respiratory tract infections in children, due to the difficulties in obtaining a...
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| Format: | Article |
| Language: | Russian |
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Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
2024-09-01
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| Series: | Клиническая микробиология и антимикробная химиотерапия |
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| Online Access: | https://cmac-journal.ru/publication/2024/3/cmac-2024-t26-n3-p274/cmac-2024-t26-n3-p274.pdf |
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| author | Matsvay A.D. Bezrukov V.M. Nikolaeva P.A. Stetsenko I.F. Nurmukanova V.A. Dikaya G.S. Gordukova M.A. Galeeva E.V. Shipulin G.A. |
| author_facet | Matsvay A.D. Bezrukov V.M. Nikolaeva P.A. Stetsenko I.F. Nurmukanova V.A. Dikaya G.S. Gordukova M.A. Galeeva E.V. Shipulin G.A. |
| author_sort | Matsvay A.D. |
| collection | DOAJ |
| description | Objective.
In 2023–2014, a significant increase in the incidence of pneumonia caused by M. pneumoniae has been reported in Russia and China. Despite being one of the primary causative agents of community-acquired lower respiratory tract infections in children, due to the difficulties in obtaining a culture of this pathogen, the amount of genomic data on M. pneumoniae is currently extremely limited, with fewer than 300 genomic sequences available, of which only 79 are complete. Until this study, no genomic data for isolates relevant to the Russian Federation were available.
Materials and Methods.
Using biological material from patients with confirmed mycoplasma infection, cultures of M. pneumoniae were obtained, for which whole-genome sequencing was carried out, followed by assembly and annotation of the pathogen genome.
Results.
We have obtained and characterized three complete circular genome sequences of M. pneumoniae, first described for Russia. Utilizing all genomic sequences of M. pneumoniae available in public databases, we conducted a phylogenetic analysis as well as a comparative analysis of clinically significant genes, revealing previously undescribed mutations in cytoadherence proteins.
Conclusions.
In this study, we characterized the first full genome sequences of M. pneumoniae isolated in the Russian Federation, as well as provided detailed methods for cultivating the pathogen and the data processing process, which can be used to further expand the amount of available data on the genetic structure of the pathogen. |
| format | Article |
| id | doaj-art-be75fa8c1a8144acb10aafccd8f0da31 |
| institution | Kabale University |
| issn | 1684-4386 2686-9586 |
| language | Russian |
| publishDate | 2024-09-01 |
| publisher | Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy |
| record_format | Article |
| series | Клиническая микробиология и антимикробная химиотерапия |
| spelling | doaj-art-be75fa8c1a8144acb10aafccd8f0da312025-01-14T06:34:16ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862024-09-0126327428410.36488/cmac.2024.3.274-284cmac-2024-t26-n3-p274Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in RussiaMatsvay A.D.0Bezrukov V.M.1Nikolaeva P.A.2Stetsenko I.F.3Nurmukanova V.A.4Dikaya G.S.5Gordukova M.A.6Galeeva E.V.7Shipulin G.A.8Federal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, Moscow, Russia“ArchiMed Clinic of New Medical Technologies, LLC”, Moscow, RussiaFederal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, Moscow, RussiaFederal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, Moscow, RussiaFederal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, Moscow, RussiaFederal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, Moscow, Russia“G. Speransky Children’s Hospital No. 9“, Moscow, Russia“G. Speransky Children’s Hospital No. 9“, Moscow, RussiaFederal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, Moscow, RussiaObjective. In 2023–2014, a significant increase in the incidence of pneumonia caused by M. pneumoniae has been reported in Russia and China. Despite being one of the primary causative agents of community-acquired lower respiratory tract infections in children, due to the difficulties in obtaining a culture of this pathogen, the amount of genomic data on M. pneumoniae is currently extremely limited, with fewer than 300 genomic sequences available, of which only 79 are complete. Until this study, no genomic data for isolates relevant to the Russian Federation were available. Materials and Methods. Using biological material from patients with confirmed mycoplasma infection, cultures of M. pneumoniae were obtained, for which whole-genome sequencing was carried out, followed by assembly and annotation of the pathogen genome. Results. We have obtained and characterized three complete circular genome sequences of M. pneumoniae, first described for Russia. Utilizing all genomic sequences of M. pneumoniae available in public databases, we conducted a phylogenetic analysis as well as a comparative analysis of clinically significant genes, revealing previously undescribed mutations in cytoadherence proteins. Conclusions. In this study, we characterized the first full genome sequences of M. pneumoniae isolated in the Russian Federation, as well as provided detailed methods for cultivating the pathogen and the data processing process, which can be used to further expand the amount of available data on the genetic structure of the pathogen.https://cmac-journal.ru/publication/2024/3/cmac-2024-t26-n3-p274/cmac-2024-t26-n3-p274.pdfpneumoniamycoplasmagenomicsphylogenyrussia |
| spellingShingle | Matsvay A.D. Bezrukov V.M. Nikolaeva P.A. Stetsenko I.F. Nurmukanova V.A. Dikaya G.S. Gordukova M.A. Galeeva E.V. Shipulin G.A. Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia Клиническая микробиология и антимикробная химиотерапия pneumonia mycoplasma genomics phylogeny russia |
| title | Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia |
| title_full | Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia |
| title_fullStr | Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia |
| title_full_unstemmed | Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia |
| title_short | Cultivation and molecular characterization of complete genome sequences of M. pneumoniae isolated in Russia |
| title_sort | cultivation and molecular characterization of complete genome sequences of m pneumoniae isolated in russia |
| topic | pneumonia mycoplasma genomics phylogeny russia |
| url | https://cmac-journal.ru/publication/2024/3/cmac-2024-t26-n3-p274/cmac-2024-t26-n3-p274.pdf |
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