Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia
Background. Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification. We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia. Methods. We retrospectively reviewed 467 mNGS results and...
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| Format: | Article |
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Wiley
2020-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/6839103 |
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| author | Xu Chen Shuizi Ding Cheng Lei Jieli Qin Ting Guo Danhui Yang Min Yang Jie Qing Wenlong He Min Song Yan Zhang Huihui Zeng Qingwu Qin Lizhen Yang Yingjiao Long Yan Chen Bingyin Ma Ruoyun Ouyang Ping Chen Hong Luo |
| author_facet | Xu Chen Shuizi Ding Cheng Lei Jieli Qin Ting Guo Danhui Yang Min Yang Jie Qing Wenlong He Min Song Yan Zhang Huihui Zeng Qingwu Qin Lizhen Yang Yingjiao Long Yan Chen Bingyin Ma Ruoyun Ouyang Ping Chen Hong Luo |
| author_sort | Xu Chen |
| collection | DOAJ |
| description | Background. Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification. We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia. Methods. We retrospectively reviewed 467 mNGS results and assessed the diagnostic performance of paired blood and BALF mNGS in 39 patients with pneumonia. Results. For bacteria and fungi, 16 patients had culture-confirmed pathogen diagnosis, while 13 patients were culture-negative. BALF mNGS was more sensitive than blood mNGS (81.3% vs. 25.0%, p=0.003), and the specificity in BALF and blood mNGS was not statistically significant different (76.9% vs. 84.6%, p=0.317). For 10 patients without culture test, treatments were changed in 2 patients. For viruses, Epstein-Barr virus was positive in blood mNGS in 9 patients. Human adenovirus was detected in both BALF and blood mNGS in 3 patients. Conclusion. Our study suggests that BALF mNGS is more sensitive than blood mNGS in detecting bacteria and fungi, but blood also has advantages to identify the pathogens of pneumonia, especially for some viruses. |
| format | Article |
| id | doaj-art-be698973ebf74a06b60ec15cfb816552 |
| institution | DOAJ |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-be698973ebf74a06b60ec15cfb8165522025-08-20T03:23:01ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932020-01-01202010.1155/2020/68391036839103Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in PneumoniaXu Chen0Shuizi Ding1Cheng Lei2Jieli Qin3Ting Guo4Danhui Yang5Min Yang6Jie Qing7Wenlong He8Min Song9Yan Zhang10Huihui Zeng11Qingwu Qin12Lizhen Yang13Yingjiao Long14Yan Chen15Bingyin Ma16Ruoyun Ouyang17Ping Chen18Hong Luo19Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaBGI Genomics, BGI-Shenzhen, Shenzhen 518083, ChinaTianjin Medical Laboratory, BGI-Tianjin, BGI-Shenzhen, Tianjin, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaBackground. Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification. We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia. Methods. We retrospectively reviewed 467 mNGS results and assessed the diagnostic performance of paired blood and BALF mNGS in 39 patients with pneumonia. Results. For bacteria and fungi, 16 patients had culture-confirmed pathogen diagnosis, while 13 patients were culture-negative. BALF mNGS was more sensitive than blood mNGS (81.3% vs. 25.0%, p=0.003), and the specificity in BALF and blood mNGS was not statistically significant different (76.9% vs. 84.6%, p=0.317). For 10 patients without culture test, treatments were changed in 2 patients. For viruses, Epstein-Barr virus was positive in blood mNGS in 9 patients. Human adenovirus was detected in both BALF and blood mNGS in 3 patients. Conclusion. Our study suggests that BALF mNGS is more sensitive than blood mNGS in detecting bacteria and fungi, but blood also has advantages to identify the pathogens of pneumonia, especially for some viruses.http://dx.doi.org/10.1155/2020/6839103 |
| spellingShingle | Xu Chen Shuizi Ding Cheng Lei Jieli Qin Ting Guo Danhui Yang Min Yang Jie Qing Wenlong He Min Song Yan Zhang Huihui Zeng Qingwu Qin Lizhen Yang Yingjiao Long Yan Chen Bingyin Ma Ruoyun Ouyang Ping Chen Hong Luo Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia |
| title_full | Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia |
| title_fullStr | Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia |
| title_full_unstemmed | Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia |
| title_short | Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia |
| title_sort | blood and bronchoalveolar lavage fluid metagenomic next generation sequencing in pneumonia |
| url | http://dx.doi.org/10.1155/2020/6839103 |
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