Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction

Background/Objectives: Myocardial infarction (MI) and diabetes pose significant health challenges globally, necessitating the development of innovative medication strategies to improve outcomes in affected populations. This research aimed to determine the defensive impact of ferulic acid (FA) agains...

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Main Authors: Sarah Ouda Bekheit, Eman Kolieb, El-Sayed E. El-Awady, Maha Abdullah Alwaili, Afaf Alharthi, Dina M. Khodeer
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/18/3/319
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author Sarah Ouda Bekheit
Eman Kolieb
El-Sayed E. El-Awady
Maha Abdullah Alwaili
Afaf Alharthi
Dina M. Khodeer
author_facet Sarah Ouda Bekheit
Eman Kolieb
El-Sayed E. El-Awady
Maha Abdullah Alwaili
Afaf Alharthi
Dina M. Khodeer
author_sort Sarah Ouda Bekheit
collection DOAJ
description Background/Objectives: Myocardial infarction (MI) and diabetes pose significant health challenges globally, necessitating the development of innovative medication strategies to improve outcomes in affected populations. This research aimed to determine the defensive impact of ferulic acid (FA) against isoproterenol-induced myocardial infarction (MI) in diabetic rats. Methods: A group of male rats was partitioned into five distinct groups: control group, diabetic group, diabetic + MI, diabetic + MI + 20 mg/kg FA, and diabetic + MI + 40 mg/kg FA. The experimental groups received isoproterenol (ISO) subcutaneously at a dosage of 50 mg/kg body weight for two consecutive days. Results: The outcome was severe cardiac toxicity, as shown by changes in electrocardiogram (ECG) rhythm and a substantial increase in blood cardiac enzymes such as creatinine kinase (CK-MB), cardiac troponin I (cTnI), and lactate dehydrogenase (LDH). Additionally, there was a surge in inflammatory cytokines, like tumor necrosis factor-alpha (TNF-α), and a disruption of the antioxidant system, evidenced by a rise in malondialdehyde (MDA) content. Moreover, there was a rise in cardiac receptor of advanced glycation end products (RAGE). Treatment with FA with escalating dosages of 20 and 40 mg/kg b.w. effectively mitigated changes in serum cardiac enzymes and improved the cellular architecture, which was evaluated by histopathological examination. Conclusions: In conclusion, in a dose-dependent manner, FA successfully showed a cardioprotective effect against ISO-induced cardiac toxicity in diabetic rats, as shown by the improvement in ECG findings, normalization of serum cardiac biomarkers, and augmentation of the endogenous antioxidant system. Therefore, the aforementioned data indicate that ferulic acid may potentially have a protective effect on MI patients who have diabetes mellitus.
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spelling doaj-art-be61eb45441842298338976223dfe7cd2025-08-20T01:49:05ZengMDPI AGPharmaceuticals1424-82472025-02-0118331910.3390/ph18030319Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial InfarctionSarah Ouda Bekheit0Eman Kolieb1El-Sayed E. El-Awady2Maha Abdullah Alwaili3Afaf Alharthi4Dina M. Khodeer5Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptDepartment of Medical Physiology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptBackground/Objectives: Myocardial infarction (MI) and diabetes pose significant health challenges globally, necessitating the development of innovative medication strategies to improve outcomes in affected populations. This research aimed to determine the defensive impact of ferulic acid (FA) against isoproterenol-induced myocardial infarction (MI) in diabetic rats. Methods: A group of male rats was partitioned into five distinct groups: control group, diabetic group, diabetic + MI, diabetic + MI + 20 mg/kg FA, and diabetic + MI + 40 mg/kg FA. The experimental groups received isoproterenol (ISO) subcutaneously at a dosage of 50 mg/kg body weight for two consecutive days. Results: The outcome was severe cardiac toxicity, as shown by changes in electrocardiogram (ECG) rhythm and a substantial increase in blood cardiac enzymes such as creatinine kinase (CK-MB), cardiac troponin I (cTnI), and lactate dehydrogenase (LDH). Additionally, there was a surge in inflammatory cytokines, like tumor necrosis factor-alpha (TNF-α), and a disruption of the antioxidant system, evidenced by a rise in malondialdehyde (MDA) content. Moreover, there was a rise in cardiac receptor of advanced glycation end products (RAGE). Treatment with FA with escalating dosages of 20 and 40 mg/kg b.w. effectively mitigated changes in serum cardiac enzymes and improved the cellular architecture, which was evaluated by histopathological examination. Conclusions: In conclusion, in a dose-dependent manner, FA successfully showed a cardioprotective effect against ISO-induced cardiac toxicity in diabetic rats, as shown by the improvement in ECG findings, normalization of serum cardiac biomarkers, and augmentation of the endogenous antioxidant system. Therefore, the aforementioned data indicate that ferulic acid may potentially have a protective effect on MI patients who have diabetes mellitus.https://www.mdpi.com/1424-8247/18/3/319advanced glycation end products and their receptorsdiabetes mellitusferulic acidisoproterenolmyocardial infarctionoxidative stress
spellingShingle Sarah Ouda Bekheit
Eman Kolieb
El-Sayed E. El-Awady
Maha Abdullah Alwaili
Afaf Alharthi
Dina M. Khodeer
Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction
Pharmaceuticals
advanced glycation end products and their receptors
diabetes mellitus
ferulic acid
isoproterenol
myocardial infarction
oxidative stress
title Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction
title_full Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction
title_fullStr Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction
title_full_unstemmed Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction
title_short Cardioprotective Effects of Ferulic Acid Through Inhibition of Advanced Glycation End Products in Diabetic Rats with Isoproterenol-Induced Myocardial Infarction
title_sort cardioprotective effects of ferulic acid through inhibition of advanced glycation end products in diabetic rats with isoproterenol induced myocardial infarction
topic advanced glycation end products and their receptors
diabetes mellitus
ferulic acid
isoproterenol
myocardial infarction
oxidative stress
url https://www.mdpi.com/1424-8247/18/3/319
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