Local de novo assembly of RAD paired-end contigs using short sequencing reads.

Local de novo assembly of RAD paired-end contigs using short sequencing reads.

Despite the power of massively parallel sequencing platforms, a drawback is the short length of the sequence reads produced. We demonstrate that short reads can be locally assembled into longer contigs using paired-end sequencing of restriction-site associated DNA (RAD-PE) fragments. We use this RAD...

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Bibliographic Details
Main Authors: Paul D Etter, Jessica L Preston, Susan Bassham, William A Cresko, Eric A Johnson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-04-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018561&type=printable
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