Capturing microalgae within aerosols provides carbon capture bio-functionality

Climate change due to the greenhouse effect poses arguably the greatest challenge to humanity. Addressing the sources of CO2 and reducing current atmospheric levels is the paramount task for scientists and engineers. Carbon capture with storage or utilization technologies are key to achieving this g...

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Main Authors: Elbaraa Elghazy, Matt M.J Davies, Nicholas T.H Farr, Cornelia Rodenburg, Jon R. Willmott, Jagroop Pandhal
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Journal of CO2 Utilization
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Online Access:http://www.sciencedirect.com/science/article/pii/S2212982025000083
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author Elbaraa Elghazy
Matt M.J Davies
Nicholas T.H Farr
Cornelia Rodenburg
Jon R. Willmott
Jagroop Pandhal
author_facet Elbaraa Elghazy
Matt M.J Davies
Nicholas T.H Farr
Cornelia Rodenburg
Jon R. Willmott
Jagroop Pandhal
author_sort Elbaraa Elghazy
collection DOAJ
description Climate change due to the greenhouse effect poses arguably the greatest challenge to humanity. Addressing the sources of CO2 and reducing current atmospheric levels is the paramount task for scientists and engineers. Carbon capture with storage or utilization technologies are key to achieving this goal. Biological carbon fixation is an effective method of converting pollutant CO2 into usable biochemicals for industrial applications. Inspired by recent evidence that 95 % of CO2 from aerosol emissions from an Australian forest fire was captured by algae in the Southern Ocean, as well as the ability of algae to be transported within aerosols, we propose a novel technique for CO2 sequestration based on creating aerosols containing metabolically active cyanobacteria. Using aerosols as a microenvironment for Synechocystis cells enables a significant increase in gas-liquid-interfacial-surface-area while reducing the volume of water required. We utilize electron microscopy and hyperspectral microscopy to assess the effects of aerosolization and high CO2 concentrations on microbial cell viability. Additionally, we implemented highspeed imaging and oil immersion microscopy to determine the effectiveness of the aerosolization technique for forming aerosols and optimizing process parameters. We show that 1 % CO2 (v/v) is ideal for CO2 capture, where cell stress was minimized. Using cell densities of 1.2 × 108 cell/mL was the most efficient in terms of the number of cells aerosolized when compared to the input cell density. We report a 6-fold increase in carbon fixation rates (gCO2 g−1 biomass hr−1) over alternative popular cultivation techniques such as bubble columns.
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spelling doaj-art-be5cfd35ac7d43d692276139cf26dd6c2025-08-20T02:13:48ZengElsevierJournal of CO2 Utilization2212-98392025-02-019210302410.1016/j.jcou.2025.103024Capturing microalgae within aerosols provides carbon capture bio-functionalityElbaraa Elghazy0Matt M.J Davies1Nicholas T.H Farr2Cornelia Rodenburg3Jon R. Willmott4Jagroop Pandhal5School of Chemical, Materials and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom; Department of Construction and Building Engineering, Arab Academy for Science, Technology, and Maritime Transport, Cairo, EgyptSchool of Electronic and Electrical Engineering, The University of Sheffield, Sheffield, United KingdomSchool of Chemical, Materials and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom; Insigneo Institute for in silico Medicine, University of Sheffield, Sheffield, United KingdomSchool of Chemical, Materials and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom; Insigneo Institute for in silico Medicine, University of Sheffield, Sheffield, United KingdomSchool of Electronic and Electrical Engineering, The University of Sheffield, Sheffield, United KingdomSchool of Chemical, Materials and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom; Corresponding author.Climate change due to the greenhouse effect poses arguably the greatest challenge to humanity. Addressing the sources of CO2 and reducing current atmospheric levels is the paramount task for scientists and engineers. Carbon capture with storage or utilization technologies are key to achieving this goal. Biological carbon fixation is an effective method of converting pollutant CO2 into usable biochemicals for industrial applications. Inspired by recent evidence that 95 % of CO2 from aerosol emissions from an Australian forest fire was captured by algae in the Southern Ocean, as well as the ability of algae to be transported within aerosols, we propose a novel technique for CO2 sequestration based on creating aerosols containing metabolically active cyanobacteria. Using aerosols as a microenvironment for Synechocystis cells enables a significant increase in gas-liquid-interfacial-surface-area while reducing the volume of water required. We utilize electron microscopy and hyperspectral microscopy to assess the effects of aerosolization and high CO2 concentrations on microbial cell viability. Additionally, we implemented highspeed imaging and oil immersion microscopy to determine the effectiveness of the aerosolization technique for forming aerosols and optimizing process parameters. We show that 1 % CO2 (v/v) is ideal for CO2 capture, where cell stress was minimized. Using cell densities of 1.2 × 108 cell/mL was the most efficient in terms of the number of cells aerosolized when compared to the input cell density. We report a 6-fold increase in carbon fixation rates (gCO2 g−1 biomass hr−1) over alternative popular cultivation techniques such as bubble columns.http://www.sciencedirect.com/science/article/pii/S2212982025000083AerosolCO2 CaptureIndustrial emissionsMicroalgaeCarbon sequestrationSustainable technology
spellingShingle Elbaraa Elghazy
Matt M.J Davies
Nicholas T.H Farr
Cornelia Rodenburg
Jon R. Willmott
Jagroop Pandhal
Capturing microalgae within aerosols provides carbon capture bio-functionality
Journal of CO2 Utilization
Aerosol
CO2 Capture
Industrial emissions
Microalgae
Carbon sequestration
Sustainable technology
title Capturing microalgae within aerosols provides carbon capture bio-functionality
title_full Capturing microalgae within aerosols provides carbon capture bio-functionality
title_fullStr Capturing microalgae within aerosols provides carbon capture bio-functionality
title_full_unstemmed Capturing microalgae within aerosols provides carbon capture bio-functionality
title_short Capturing microalgae within aerosols provides carbon capture bio-functionality
title_sort capturing microalgae within aerosols provides carbon capture bio functionality
topic Aerosol
CO2 Capture
Industrial emissions
Microalgae
Carbon sequestration
Sustainable technology
url http://www.sciencedirect.com/science/article/pii/S2212982025000083
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AT nicholasthfarr capturingmicroalgaewithinaerosolsprovidescarboncapturebiofunctionality
AT corneliarodenburg capturingmicroalgaewithinaerosolsprovidescarboncapturebiofunctionality
AT jonrwillmott capturingmicroalgaewithinaerosolsprovidescarboncapturebiofunctionality
AT jagrooppandhal capturingmicroalgaewithinaerosolsprovidescarboncapturebiofunctionality