Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles
Secretory phospholipase A2, an enzyme that exhibits substantial immunological activity, was measured in the serum of three species of diverse West African crocodiles. Incubation of different volumes of crocodile serum with bacteria labeled with a fluorescent fatty acid in the sn-2 position of membra...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2011/925012 |
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| author | Mark Merchant Kate Juneau Jared Gemillion Rodolfo Falconi Aaron Doucet Matthew H. Shirley |
| author_facet | Mark Merchant Kate Juneau Jared Gemillion Rodolfo Falconi Aaron Doucet Matthew H. Shirley |
| author_sort | Mark Merchant |
| collection | DOAJ |
| description | Secretory phospholipase A2, an enzyme that exhibits substantial immunological activity, was measured in the serum of three species of diverse West African crocodiles. Incubation of different volumes of crocodile serum with bacteria labeled with a fluorescent fatty acid in the sn-2 position of membrane lipids resulted in a volume-dependent liberation of fluorescent probe. Serum from the Nile crocodile (Crocodylus niloticus) exhibited slightly higher activity than that of the slender-snouted crocodile (Mecistops cataphractus) and the African dwarf crocodile (Osteolaemus tetraspis). Product formation was inhibited by BPB, a specific PLA2 inhibitor, confirming that the activity was a direct result of the presence of serum PLA2. Kinetic analysis showed that C. niloticus serum produced product more rapidly than M. cataphractus or O. tetraspis. Serum from all three species exhibited temperature-dependent PLA2 activities but with slightly different thermal profiles. All three crocodilian species showed high levels of activity against eight different species of bacteria. |
| format | Article |
| id | doaj-art-be421c780aed4060862b50dd04305c8e |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-be421c780aed4060862b50dd04305c8e2025-02-03T06:42:20ZengWileyBiochemistry Research International2090-22472090-22552011-01-01201110.1155/2011/925012925012Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African CrocodilesMark Merchant0Kate Juneau1Jared Gemillion2Rodolfo Falconi3Aaron Doucet4Matthew H. Shirley5Department of Chemistry, McNeese State University, 450 Beauregard, Kirkman Hall 221A, Lake Charles, LA 70609, USADepartment of Chemistry, McNeese State University, 450 Beauregard, Kirkman Hall 221A, Lake Charles, LA 70609, USADepartment of Chemistry, McNeese State University, 450 Beauregard, Kirkman Hall 221A, Lake Charles, LA 70609, USADepartment of Chemistry, McNeese State University, 450 Beauregard, Kirkman Hall 221A, Lake Charles, LA 70609, USADepartment of Chemistry, McNeese State University, 450 Beauregard, Kirkman Hall 221A, Lake Charles, LA 70609, USADepartment of Wildlife Ecology & Conservation, University of Florida, Gainesville, FL, USASecretory phospholipase A2, an enzyme that exhibits substantial immunological activity, was measured in the serum of three species of diverse West African crocodiles. Incubation of different volumes of crocodile serum with bacteria labeled with a fluorescent fatty acid in the sn-2 position of membrane lipids resulted in a volume-dependent liberation of fluorescent probe. Serum from the Nile crocodile (Crocodylus niloticus) exhibited slightly higher activity than that of the slender-snouted crocodile (Mecistops cataphractus) and the African dwarf crocodile (Osteolaemus tetraspis). Product formation was inhibited by BPB, a specific PLA2 inhibitor, confirming that the activity was a direct result of the presence of serum PLA2. Kinetic analysis showed that C. niloticus serum produced product more rapidly than M. cataphractus or O. tetraspis. Serum from all three species exhibited temperature-dependent PLA2 activities but with slightly different thermal profiles. All three crocodilian species showed high levels of activity against eight different species of bacteria.http://dx.doi.org/10.1155/2011/925012 |
| spellingShingle | Mark Merchant Kate Juneau Jared Gemillion Rodolfo Falconi Aaron Doucet Matthew H. Shirley Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles Biochemistry Research International |
| title | Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles |
| title_full | Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles |
| title_fullStr | Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles |
| title_full_unstemmed | Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles |
| title_short | Characterization of Serum Phospholipase A2 Activity in Three Diverse Species of West African Crocodiles |
| title_sort | characterization of serum phospholipase a2 activity in three diverse species of west african crocodiles |
| url | http://dx.doi.org/10.1155/2011/925012 |
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