Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway

Background Cervical cancer remains a global health challenge. The identification of new immunotherapeutic targets may provide a promising platform for advancing cervical cancer treatment.Objective This study aims to investigate the role of CUB domain-containing protein 1 (CDCP1) in cervical cancer p...

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Main Authors: Jie Li, Wei Wang, Chunyu Zhang, Tianyu Liu, Li Yuan, Yili Chen, Qiqiao Du, Junxiu Liu, Jiaming Huang, Yanchun Liang, Shuzhong Yao, Hua Huang, Qiuwen Mai, Qiaojian Zou, Yuwen Pan, Yuandong Liao, Shuhang Qin, Yijia Zhou, Chao Yun Pan
Format: Article
Language:English
Published: BMJ Publishing Group 2024-10-01
Series:Journal for ImmunoTherapy of Cancer
Online Access:https://jitc.bmj.com/content/12/10/e009416.full
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author Jie Li
Wei Wang
Chunyu Zhang
Tianyu Liu
Li Yuan
Yili Chen
Qiqiao Du
Junxiu Liu
Jiaming Huang
Yanchun Liang
Shuzhong Yao
Hua Huang
Qiuwen Mai
Qiaojian Zou
Yuwen Pan
Yuandong Liao
Shuhang Qin
Yijia Zhou
Chao Yun Pan
author_facet Jie Li
Wei Wang
Chunyu Zhang
Tianyu Liu
Li Yuan
Yili Chen
Qiqiao Du
Junxiu Liu
Jiaming Huang
Yanchun Liang
Shuzhong Yao
Hua Huang
Qiuwen Mai
Qiaojian Zou
Yuwen Pan
Yuandong Liao
Shuhang Qin
Yijia Zhou
Chao Yun Pan
author_sort Jie Li
collection DOAJ
description Background Cervical cancer remains a global health challenge. The identification of new immunotherapeutic targets may provide a promising platform for advancing cervical cancer treatment.Objective This study aims to investigate the role of CUB domain-containing protein 1 (CDCP1) in cervical cancer progression and evaluate its potential as a therapeutic target.Methods We performed comprehensive analyses using patient cohorts and preclinical models to examine the association between CDCP1 expression and cervical cancer prognosis. Then in immunodeficient and immunocompetent mouse models, we further investigated the impact of CDCP1 on the tumor immune microenvironment, focusing on its effects on tumor-infiltrating T cells, including cytotoxic T lymphocytes (CTLs) and regulatory T cells (Tregs). Mechanistic studies were performed to elucidate the pathways involved in CDCP1-mediated immune modulation, in particular its interaction with the T cell receptor CD6 and the activation of the JAK-STAT signaling pathway.Results Our results show that CDCP1 overexpression is associated with poor prognosis and T cell infliction in cervical cancer. Specifically, it affects the activity of CTLs and Tregs. Mechanistically, CDCP1 binds to CD6 and inhibits the JAK-STAT pathway of T cells. The study further demonstrates that targeting CDCP1 with the inhibitor 8-prenylnaringenin (8PN) effectively suppresses tumor growth in vivo and enhances antitumor immunity.Conclusions CDCP1 plays a critical role in cervical cancer progression by modulating the tumor immune microenvironment. Targeting CDCP1 offers a promising therapeutic strategy to improve the outcome of patients with cervical cancer.
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spelling doaj-art-be3c323be8594ca0a1d24cfc7d4cb2852025-08-20T02:58:01ZengBMJ Publishing GroupJournal for ImmunoTherapy of Cancer2051-14262024-10-01121010.1136/jitc-2024-009416Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathwayJie Li0Wei Wang1Chunyu Zhang2Tianyu Liu3Li Yuan4Yili Chen5Qiqiao Du6Junxiu Liu7Jiaming Huang8Yanchun Liang9Shuzhong Yao10Hua Huang11Qiuwen Mai12Qiaojian Zou13Yuwen Pan14Yuandong Liao15Shuhang Qin16Yijia Zhou17Chao Yun Pan18associate director for RWEDepartment of Orthopedics, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaGuangdong Provincial Clinical Research Center for Obstetrical and Gynecological Diseases, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Neurology, Dazhou Central Hospital, Dazhou, Sichuan, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Obstetrics and Gynecology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, Guangdong, ChinaDepartment of Biochemistry, Sun Yat-Sen University, Guangzhou, Guangdong, ChinaBackground Cervical cancer remains a global health challenge. The identification of new immunotherapeutic targets may provide a promising platform for advancing cervical cancer treatment.Objective This study aims to investigate the role of CUB domain-containing protein 1 (CDCP1) in cervical cancer progression and evaluate its potential as a therapeutic target.Methods We performed comprehensive analyses using patient cohorts and preclinical models to examine the association between CDCP1 expression and cervical cancer prognosis. Then in immunodeficient and immunocompetent mouse models, we further investigated the impact of CDCP1 on the tumor immune microenvironment, focusing on its effects on tumor-infiltrating T cells, including cytotoxic T lymphocytes (CTLs) and regulatory T cells (Tregs). Mechanistic studies were performed to elucidate the pathways involved in CDCP1-mediated immune modulation, in particular its interaction with the T cell receptor CD6 and the activation of the JAK-STAT signaling pathway.Results Our results show that CDCP1 overexpression is associated with poor prognosis and T cell infliction in cervical cancer. Specifically, it affects the activity of CTLs and Tregs. Mechanistically, CDCP1 binds to CD6 and inhibits the JAK-STAT pathway of T cells. The study further demonstrates that targeting CDCP1 with the inhibitor 8-prenylnaringenin (8PN) effectively suppresses tumor growth in vivo and enhances antitumor immunity.Conclusions CDCP1 plays a critical role in cervical cancer progression by modulating the tumor immune microenvironment. Targeting CDCP1 offers a promising therapeutic strategy to improve the outcome of patients with cervical cancer.https://jitc.bmj.com/content/12/10/e009416.full
spellingShingle Jie Li
Wei Wang
Chunyu Zhang
Tianyu Liu
Li Yuan
Yili Chen
Qiqiao Du
Junxiu Liu
Jiaming Huang
Yanchun Liang
Shuzhong Yao
Hua Huang
Qiuwen Mai
Qiaojian Zou
Yuwen Pan
Yuandong Liao
Shuhang Qin
Yijia Zhou
Chao Yun Pan
Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway
Journal for ImmunoTherapy of Cancer
title Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway
title_full Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway
title_fullStr Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway
title_full_unstemmed Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway
title_short Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway
title_sort targeting cdcp1 boost cd8 t cells mediated cytotoxicity in cervical cancer via the jak stat signaling pathway
url https://jitc.bmj.com/content/12/10/e009416.full
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