Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout

Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout

Bacillus licheniformis is a significant industrial microorganism. Traditional gene editing techniques relying on homologous recombination often exhibit low efficiency due to their reliance on resistance genes. Additionally, the established CRISPR gene editing technology, utilizing Cas9 endonuclease,...

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Bibliographic Details
Main Authors: Suxin Liu, Fengxu Xiao, Youran Li, Yupeng Zhang, Yanling Wang, Guiyang Shi
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-03-01
Series:Synthetic and Systems Biotechnology
Subjects:
Bacillus licheniformis
Gene editing
CRISPR-Cpf1
MCherry
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X24001133
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