Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta)
Yeasts are an important component of insect gut microbial content, playing roles such as degradation of polymers and toxic compounds, biological control, and hormone, vitamin, and digestive enzyme production. The xylophagous beetle gut is a hyperdiverse habitat and a potential source of new species...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/5346741 |
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| author | Italo Thiago Silveira Rocha Matos Enedina Nogueira Assunção Edson Junior do Carmo Verena Makaren Soares Spartaco Astolfi-Filho |
| author_facet | Italo Thiago Silveira Rocha Matos Enedina Nogueira Assunção Edson Junior do Carmo Verena Makaren Soares Spartaco Astolfi-Filho |
| author_sort | Italo Thiago Silveira Rocha Matos |
| collection | DOAJ |
| description | Yeasts are an important component of insect gut microbial content, playing roles such as degradation of polymers and toxic compounds, biological control, and hormone, vitamin, and digestive enzyme production. The xylophagous beetle gut is a hyperdiverse habitat and a potential source of new species with industrial abilities such as enzyme production, pentose fermentation, and biodetoxification. In this work, samples of Veturius transversus (Passalidae, Coleoptera, and Insecta) were collected from the Central Amazon Rainforest. Their guts were dissected and a total of 20 microbial colonies were isolated using sugarcane bagasse hemicellulosic hydrolysate. They were identified as having 10 distinct biochemical profiles, and genetic analysis allowed identification as three clades in the genera Candida, Williopsis, and Geotrichum. All colonies were able to assimilate D-xylose and 18 were able to produce xylitol, especially a strain of Geotrichum, with a maximum yield of 0.502 g·g−1. These results agree with a previous prediction that the microbial community associated with xylophagous insects is a promising source of species of biotechnological interest. |
| format | Article |
| id | doaj-art-be37c1f32b504d71bcd86ba49a2b04c3 |
| institution | DOAJ |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-be37c1f32b504d71bcd86ba49a2b04c32025-08-20T03:23:03ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/53467415346741Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta)Italo Thiago Silveira Rocha Matos0Enedina Nogueira Assunção1Edson Junior do Carmo2Verena Makaren Soares3Spartaco Astolfi-Filho4Departamento de Genética, Universidade Federal do Amazonas, Av. Gal. Rodrigo Octávio, 3000 Manaus, AM, BrazilDepartamento de Genética, Universidade Federal do Amazonas, Av. Gal. Rodrigo Octávio, 3000 Manaus, AM, BrazilDepartamento de Genética, Universidade Federal do Amazonas, Av. Gal. Rodrigo Octávio, 3000 Manaus, AM, BrazilDepartamento de Genética, Universidade Federal do Amazonas, Av. Gal. Rodrigo Octávio, 3000 Manaus, AM, BrazilDepartamento de Genética, Universidade Federal do Amazonas, Av. Gal. Rodrigo Octávio, 3000 Manaus, AM, BrazilYeasts are an important component of insect gut microbial content, playing roles such as degradation of polymers and toxic compounds, biological control, and hormone, vitamin, and digestive enzyme production. The xylophagous beetle gut is a hyperdiverse habitat and a potential source of new species with industrial abilities such as enzyme production, pentose fermentation, and biodetoxification. In this work, samples of Veturius transversus (Passalidae, Coleoptera, and Insecta) were collected from the Central Amazon Rainforest. Their guts were dissected and a total of 20 microbial colonies were isolated using sugarcane bagasse hemicellulosic hydrolysate. They were identified as having 10 distinct biochemical profiles, and genetic analysis allowed identification as three clades in the genera Candida, Williopsis, and Geotrichum. All colonies were able to assimilate D-xylose and 18 were able to produce xylitol, especially a strain of Geotrichum, with a maximum yield of 0.502 g·g−1. These results agree with a previous prediction that the microbial community associated with xylophagous insects is a promising source of species of biotechnological interest.http://dx.doi.org/10.1155/2017/5346741 |
| spellingShingle | Italo Thiago Silveira Rocha Matos Enedina Nogueira Assunção Edson Junior do Carmo Verena Makaren Soares Spartaco Astolfi-Filho Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta) International Journal of Microbiology |
| title | Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta) |
| title_full | Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta) |
| title_fullStr | Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta) |
| title_full_unstemmed | Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta) |
| title_short | Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta) |
| title_sort | isolation and characterization of yeasts able to assimilate sugarcane bagasse hemicellulosic hydrolysate and produce xylitol associated with veturius transversus passalidae coleoptera and insecta |
| url | http://dx.doi.org/10.1155/2017/5346741 |
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