<i>Zingiber officinale</i> Polysaccharide Silver Nanoparticles: A Study of Its Synthesis, Structure Elucidation, Antibacterial and Immunomodulatory Activities
Green-synthesized metal nanoparticles show promise in nanomedicine and material engineering. In this study, the polysaccharide of <i>Zingiber officinale</i> (ZOP) was used as a raw material. Through single-factor experiments and a response surface methodology, the optimum synthesis proto...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/15/14/1064 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Green-synthesized metal nanoparticles show promise in nanomedicine and material engineering. In this study, the polysaccharide of <i>Zingiber officinale</i> (ZOP) was used as a raw material. Through single-factor experiments and a response surface methodology, the optimum synthesis protocol of <i>Zingiber officinale</i> polysaccharide silver nanoparticles (ZOP-NPs-AgNPs) was determined as follows: V(AgNO<sub>3</sub>):V(ZOP) = 2.98:1, 59.79 °C, 3 h, pH 9, and 20 mL NaCl, achieving a 92.51% silver chelation rate. Structure analysis revealed that ZOP-NPs-AgNPs were spherical or quasi-spherical, with a particle size < 20 nm and a face-centered cubic crystal structure, which has good thermal stability. Subsequent studies explored the antibacterial and immunomodulatory effects of ZOP-NPs-AgNPs. The minimum inhibitory concentration (MIC) of ZOP-NPs-AgNPs against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> was determined to be 0.5000 mg/mL and 0.0310 mg/mL, respectively, while the minimum bactericidal concentration (MBC) was 0.5000 mg/mL and 0.0310 mg/mL, respectively. Additionally, ZOP-NPs-AgNPs significantly enhance RAW264.7 cell proliferation and phagocytosis and boost IL−1β, IL−6, NO, and TNF-α production. This confirms that ZOP can act as a green reductant and stabilizer, offering a new method for green nano-silver synthesis. This provides a sustainable way to produce antibacterial products and functional foods, and offers useful references for eco-friendly nano-silver applications. |
|---|---|
| ISSN: | 2079-4991 |