Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima
The metamorphosis of the planktonic larvae of bivalve species plays a key role in their complex life history strategies, and this process involves changes in the molecular machinery required for sessile life and the development of intricate shells. Understanding the key molecular agents and special...
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Elsevier
2024-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424004654 |
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| author | Minxin Liang Wenhao Yu Kaiyao He Qingheng Wang Yongshan Liao Ronglian Huang Dewei Cheng Yuewen Deng Zhe Zheng Chuangye Yang |
| author_facet | Minxin Liang Wenhao Yu Kaiyao He Qingheng Wang Yongshan Liao Ronglian Huang Dewei Cheng Yuewen Deng Zhe Zheng Chuangye Yang |
| author_sort | Minxin Liang |
| collection | DOAJ |
| description | The metamorphosis of the planktonic larvae of bivalve species plays a key role in their complex life history strategies, and this process involves changes in the molecular machinery required for sessile life and the development of intricate shells. Understanding the key molecular agents and special requirements in this development stage is critically important for increasing the production of larvae and promoting population recovery. Here, we cultured larvae of the giant pearl oyster Pinctada maxima, a protected bivalve species in China that is used for the commercial production of large pearls. RNA sequencing was used to identify differentially expressed genes (DEGs) before (E) and after metamorphosis (ES). A total of 8270 DEGs were identified. The up-regulation of genes related to oxidative phosphorylation at the ES stage indicate an increase in energy metabolism. The DEGs were systematically determined by conserved domain analysis. DEGs involved in apoptosis and autophagy coincided with changes in morphology and tissue reconstruction at the metamorphosis stage. In addition, the increased expression of genes related to hormone, neurotransmitter/neuropeptide receptor, and immunitye at the ES stage suggests that they play an important role in the induction of various metabolic processes and the development of the immune system following exposure to new environments. The high expression of vitamin B transporters indicates that the absorption and bioavailability of vitamin B are particularly important for the metamorphosis of P. maxima. These findings provide new insights into the factors inducing metamorphosis and the nutritional requirements of rare bivalve species, which have implications for the population restoration and breeding of bivalves. |
| format | Article |
| id | doaj-art-be24ebefe75d4a1daccf87c81090cd85 |
| institution | OA Journals |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-be24ebefe75d4a1daccf87c81090cd852025-08-20T02:38:45ZengElsevierAquaculture Reports2352-51342024-12-013910237710.1016/j.aqrep.2024.102377Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maximaMinxin Liang0Wenhao Yu1Kaiyao He2Qingheng Wang3Yongshan Liao4Ronglian Huang5Dewei Cheng6Yuewen Deng7Zhe Zheng8Chuangye Yang9Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural Resources, Guangzhou 510301, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Corresponding authors at: Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Corresponding authors at: Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.The metamorphosis of the planktonic larvae of bivalve species plays a key role in their complex life history strategies, and this process involves changes in the molecular machinery required for sessile life and the development of intricate shells. Understanding the key molecular agents and special requirements in this development stage is critically important for increasing the production of larvae and promoting population recovery. Here, we cultured larvae of the giant pearl oyster Pinctada maxima, a protected bivalve species in China that is used for the commercial production of large pearls. RNA sequencing was used to identify differentially expressed genes (DEGs) before (E) and after metamorphosis (ES). A total of 8270 DEGs were identified. The up-regulation of genes related to oxidative phosphorylation at the ES stage indicate an increase in energy metabolism. The DEGs were systematically determined by conserved domain analysis. DEGs involved in apoptosis and autophagy coincided with changes in morphology and tissue reconstruction at the metamorphosis stage. In addition, the increased expression of genes related to hormone, neurotransmitter/neuropeptide receptor, and immunitye at the ES stage suggests that they play an important role in the induction of various metabolic processes and the development of the immune system following exposure to new environments. The high expression of vitamin B transporters indicates that the absorption and bioavailability of vitamin B are particularly important for the metamorphosis of P. maxima. These findings provide new insights into the factors inducing metamorphosis and the nutritional requirements of rare bivalve species, which have implications for the population restoration and breeding of bivalves.http://www.sciencedirect.com/science/article/pii/S2352513424004654Pinctada maximaTranscriptomicSettlement and metamorphosisLarval |
| spellingShingle | Minxin Liang Wenhao Yu Kaiyao He Qingheng Wang Yongshan Liao Ronglian Huang Dewei Cheng Yuewen Deng Zhe Zheng Chuangye Yang Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima Aquaculture Reports Pinctada maxima Transcriptomic Settlement and metamorphosis Larval |
| title | Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima |
| title_full | Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima |
| title_fullStr | Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima |
| title_full_unstemmed | Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima |
| title_short | Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima |
| title_sort | transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of pinctada maxima |
| topic | Pinctada maxima Transcriptomic Settlement and metamorphosis Larval |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424004654 |
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