Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection.
<h4>Rationale</h4>Understanding mechanisms of resistance to M. tuberculosis (M.tb) infection in humans could identify novel therapeutic strategies as it has for other infectious diseases, such as HIV.<h4>Objectives</h4>To compare the early transcriptional response of M.tb-inf...
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| Format: | Article |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0175844&type=printable |
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| author | Chetan Seshadri Nafiseh Sedaghat Monica Campo Glenna Peterson Richard D Wells Gregory S Olson David R Sherman Catherine M Stein Harriet Mayanja-Kizza Ali Shojaie W Henry Boom Thomas R Hawn Tuberculosis Research Unit (TBRU) |
| author_facet | Chetan Seshadri Nafiseh Sedaghat Monica Campo Glenna Peterson Richard D Wells Gregory S Olson David R Sherman Catherine M Stein Harriet Mayanja-Kizza Ali Shojaie W Henry Boom Thomas R Hawn Tuberculosis Research Unit (TBRU) |
| author_sort | Chetan Seshadri |
| collection | DOAJ |
| description | <h4>Rationale</h4>Understanding mechanisms of resistance to M. tuberculosis (M.tb) infection in humans could identify novel therapeutic strategies as it has for other infectious diseases, such as HIV.<h4>Objectives</h4>To compare the early transcriptional response of M.tb-infected monocytes between Ugandan household contacts of tuberculosis patients who demonstrate clinical resistance to M.tb infection (cases) and matched controls with latent tuberculosis infection.<h4>Methods</h4>Cases (n = 10) and controls (n = 18) were selected from a long-term household contact study in which cases did not convert their tuberculin skin test (TST) or develop tuberculosis over two years of follow up. We obtained genome-wide transcriptional profiles of M.tb-infected peripheral blood monocytes and used Gene Set Enrichment Analysis and interaction networks to identify cellular processes associated with resistance to clinical M.tb infection.<h4>Measurements and main results</h4>We discovered gene sets associated with histone deacetylases that were differentially expressed when comparing resistant and susceptible subjects. We used small molecule inhibitors to demonstrate that histone deacetylase function is important for the pro-inflammatory response to in-vitro M.tb infection in human monocytes.<h4>Conclusions</h4>Monocytes from individuals who appear to resist clinical M.tb infection differentially activate pathways controlled by histone deacetylase in response to in-vitro M.tb infection when compared to those who are susceptible and develop latent tuberculosis. These data identify a potential cellular mechanism underlying the clinical phenomenon of resistance to M.tb infection despite known exposure to an infectious contact. |
| format | Article |
| id | doaj-art-be1f5fc0e02c4c509ccb60eacd7db1ef |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-be1f5fc0e02c4c509ccb60eacd7db1ef2025-08-20T02:03:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017584410.1371/journal.pone.0175844Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection.Chetan SeshadriNafiseh SedaghatMonica CampoGlenna PetersonRichard D WellsGregory S OlsonDavid R ShermanCatherine M SteinHarriet Mayanja-KizzaAli ShojaieW Henry BoomThomas R HawnTuberculosis Research Unit (TBRU)<h4>Rationale</h4>Understanding mechanisms of resistance to M. tuberculosis (M.tb) infection in humans could identify novel therapeutic strategies as it has for other infectious diseases, such as HIV.<h4>Objectives</h4>To compare the early transcriptional response of M.tb-infected monocytes between Ugandan household contacts of tuberculosis patients who demonstrate clinical resistance to M.tb infection (cases) and matched controls with latent tuberculosis infection.<h4>Methods</h4>Cases (n = 10) and controls (n = 18) were selected from a long-term household contact study in which cases did not convert their tuberculin skin test (TST) or develop tuberculosis over two years of follow up. We obtained genome-wide transcriptional profiles of M.tb-infected peripheral blood monocytes and used Gene Set Enrichment Analysis and interaction networks to identify cellular processes associated with resistance to clinical M.tb infection.<h4>Measurements and main results</h4>We discovered gene sets associated with histone deacetylases that were differentially expressed when comparing resistant and susceptible subjects. We used small molecule inhibitors to demonstrate that histone deacetylase function is important for the pro-inflammatory response to in-vitro M.tb infection in human monocytes.<h4>Conclusions</h4>Monocytes from individuals who appear to resist clinical M.tb infection differentially activate pathways controlled by histone deacetylase in response to in-vitro M.tb infection when compared to those who are susceptible and develop latent tuberculosis. These data identify a potential cellular mechanism underlying the clinical phenomenon of resistance to M.tb infection despite known exposure to an infectious contact.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0175844&type=printable |
| spellingShingle | Chetan Seshadri Nafiseh Sedaghat Monica Campo Glenna Peterson Richard D Wells Gregory S Olson David R Sherman Catherine M Stein Harriet Mayanja-Kizza Ali Shojaie W Henry Boom Thomas R Hawn Tuberculosis Research Unit (TBRU) Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection. PLoS ONE |
| title | Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection. |
| title_full | Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection. |
| title_fullStr | Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection. |
| title_full_unstemmed | Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection. |
| title_short | Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection. |
| title_sort | transcriptional networks are associated with resistance to mycobacterium tuberculosis infection |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0175844&type=printable |
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