Response of soybean root exudates and related metabolic pathways to low phosphorus stress.
Phosphorus (P) is an essential elemental nutrient required in high abundance for robust soybean growth and development. Low P stress negatively impacts plant physiological and biochemical processes, such as photosynthesis, respiration, and energy transfer. Soybean roots play key roles in plant adapt...
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0314256&type=printable |
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| author | Yubo Yao Hongmei Yuan Dandan Liu Lili Cheng |
| author_facet | Yubo Yao Hongmei Yuan Dandan Liu Lili Cheng |
| author_sort | Yubo Yao |
| collection | DOAJ |
| description | Phosphorus (P) is an essential elemental nutrient required in high abundance for robust soybean growth and development. Low P stress negatively impacts plant physiological and biochemical processes, such as photosynthesis, respiration, and energy transfer. Soybean roots play key roles in plant adaptive responses to P stress and other soil-related environmental stressors. Study the changes in soybean root exudates and differences in related metabolic pathways under low phosphorus stress, analyzing the response mechanism of soybean roots to phosphorus stress from the perspective of root exudates, which provide a theoretical basis for further analyzing the physiological mechanism of phosphorus stress on soybean. In this study, soybean roots were exposed to three phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress) and 31 mg/L (Normal P) for 10 days and 20 days, then root exudates were analyzed via ultra-high-performance liquid chromatography-mass spectrometry to identify effects of P stress on root metabolite profiles and associated metabolic pathways. Our results revealed that with increasing P stress severity and/or duration, soybean roots produced altered types, quantities, and increased numbers of exudate metabolites (DMs in the P1 group were primarily upregulated, whereas those in the P11 group were predominately downregulated) caused by changes in regulation of activities of numerous metabolic pathways. These pathways had functions related to environmental adaptation, energy metabolism, and scavenging of reactive oxygen species and primarily included amino acid, flavonoid, and nicotinate and nicotinamide metabolic pathways and pathways related to isoquinoline alkaloid biosynthesis, sugar catabolism, and phospholipid metabolism. These metabolites and metabolic pathways lay a foundation to support further investigations of physiological mechanisms underlying the soybean root response to P deficiency. |
| format | Article |
| id | doaj-art-be17454214e04bdfb60f431f11f1dcda |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-be17454214e04bdfb60f431f11f1dcda2025-08-20T02:58:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031425610.1371/journal.pone.0314256Response of soybean root exudates and related metabolic pathways to low phosphorus stress.Yubo YaoHongmei YuanDandan LiuLili ChengPhosphorus (P) is an essential elemental nutrient required in high abundance for robust soybean growth and development. Low P stress negatively impacts plant physiological and biochemical processes, such as photosynthesis, respiration, and energy transfer. Soybean roots play key roles in plant adaptive responses to P stress and other soil-related environmental stressors. Study the changes in soybean root exudates and differences in related metabolic pathways under low phosphorus stress, analyzing the response mechanism of soybean roots to phosphorus stress from the perspective of root exudates, which provide a theoretical basis for further analyzing the physiological mechanism of phosphorus stress on soybean. In this study, soybean roots were exposed to three phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress) and 31 mg/L (Normal P) for 10 days and 20 days, then root exudates were analyzed via ultra-high-performance liquid chromatography-mass spectrometry to identify effects of P stress on root metabolite profiles and associated metabolic pathways. Our results revealed that with increasing P stress severity and/or duration, soybean roots produced altered types, quantities, and increased numbers of exudate metabolites (DMs in the P1 group were primarily upregulated, whereas those in the P11 group were predominately downregulated) caused by changes in regulation of activities of numerous metabolic pathways. These pathways had functions related to environmental adaptation, energy metabolism, and scavenging of reactive oxygen species and primarily included amino acid, flavonoid, and nicotinate and nicotinamide metabolic pathways and pathways related to isoquinoline alkaloid biosynthesis, sugar catabolism, and phospholipid metabolism. These metabolites and metabolic pathways lay a foundation to support further investigations of physiological mechanisms underlying the soybean root response to P deficiency.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0314256&type=printable |
| spellingShingle | Yubo Yao Hongmei Yuan Dandan Liu Lili Cheng Response of soybean root exudates and related metabolic pathways to low phosphorus stress. PLoS ONE |
| title | Response of soybean root exudates and related metabolic pathways to low phosphorus stress. |
| title_full | Response of soybean root exudates and related metabolic pathways to low phosphorus stress. |
| title_fullStr | Response of soybean root exudates and related metabolic pathways to low phosphorus stress. |
| title_full_unstemmed | Response of soybean root exudates and related metabolic pathways to low phosphorus stress. |
| title_short | Response of soybean root exudates and related metabolic pathways to low phosphorus stress. |
| title_sort | response of soybean root exudates and related metabolic pathways to low phosphorus stress |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0314256&type=printable |
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