Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique
Introduction: Clostridium difficile is the most common cause of antibiotic-associated diarrhea and colitis. Several methods are available for the detection of C. difficile in stool samples. This study aimed to use glutamate dehydrogenase (GDH), toxin detection, culture and polymerase chain reaction...
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The Journal of Infection in Developing Countries
2023-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17473 |
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| author | Bakhtyar Nader Ali Ali Yahya Saeed Amir Abdulmawjood |
| author_facet | Bakhtyar Nader Ali Ali Yahya Saeed Amir Abdulmawjood |
| author_sort | Bakhtyar Nader Ali |
| collection | DOAJ |
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Introduction: Clostridium difficile is the most common cause of antibiotic-associated diarrhea and colitis. Several methods are available for the detection of C. difficile in stool samples. This study aimed to use glutamate dehydrogenase (GDH), toxin detection, culture and polymerase chain reaction (PCR) techniques for the diagnosis of this pathogen.
Methodology: A total of 300 stool samples were collected from children with hospital acquired diarrhea (HA-D), community acquired diarrhea (CA-D), and hospitalized non-diarrheic children as control with ages ranging from 6 months to 6 years (mean 3.7 ± 1.7). Each stool sample was divided into two parts; one part was tested for the enzyme GDH, toxin A and B and then cultured on selective media; and the other part for direct DNA extraction.
Results: From a total of 300 stool samples, 9 (3.0%) were positive for C. difficile by the PCR technique, 7 (7%) samples of which were from HA-D cases and 2 (2.0%) from CA-D cases; the control group samples were negative. The enzyme GDH was detected in 12 (12%) samples and toxins A and B in 8 (8%) samples from HA-D cases compared to 5 (5%) and 2 (2%), respectively from CA-D cases. Both GDH and the toxins were negative in control samples. Only 19 (19.0%) samples from HA-D cases gave suspected growth and all of these were negative by PCR.
Conclusions: Based on the results of this study, we conclude that the PCR technique is the only reliable method for the diagnosis of this pathogen.
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| format | Article |
| id | doaj-art-be0f62ff5b85411ba22cae117bc6524c |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-be0f62ff5b85411ba22cae117bc6524c2025-08-20T02:57:21ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-10-01171010.3855/jidc.17473Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction techniqueBakhtyar Nader Ali0Ali Yahya Saeed1Amir Abdulmawjood2Duhok Research Center, College of Science, University of Duhok, Duhok, IraqDepartment of Biology, College of Science, University of Duhok, Duhok, IraqInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany Introduction: Clostridium difficile is the most common cause of antibiotic-associated diarrhea and colitis. Several methods are available for the detection of C. difficile in stool samples. This study aimed to use glutamate dehydrogenase (GDH), toxin detection, culture and polymerase chain reaction (PCR) techniques for the diagnosis of this pathogen. Methodology: A total of 300 stool samples were collected from children with hospital acquired diarrhea (HA-D), community acquired diarrhea (CA-D), and hospitalized non-diarrheic children as control with ages ranging from 6 months to 6 years (mean 3.7 ± 1.7). Each stool sample was divided into two parts; one part was tested for the enzyme GDH, toxin A and B and then cultured on selective media; and the other part for direct DNA extraction. Results: From a total of 300 stool samples, 9 (3.0%) were positive for C. difficile by the PCR technique, 7 (7%) samples of which were from HA-D cases and 2 (2.0%) from CA-D cases; the control group samples were negative. The enzyme GDH was detected in 12 (12%) samples and toxins A and B in 8 (8%) samples from HA-D cases compared to 5 (5%) and 2 (2%), respectively from CA-D cases. Both GDH and the toxins were negative in control samples. Only 19 (19.0%) samples from HA-D cases gave suspected growth and all of these were negative by PCR. Conclusions: Based on the results of this study, we conclude that the PCR technique is the only reliable method for the diagnosis of this pathogen. https://jidc.org/index.php/journal/article/view/17473Clostridium difficilediarrheaculturePCR |
| spellingShingle | Bakhtyar Nader Ali Ali Yahya Saeed Amir Abdulmawjood Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique Journal of Infection in Developing Countries Clostridium difficile diarrhea culture PCR |
| title | Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique |
| title_full | Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique |
| title_fullStr | Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique |
| title_full_unstemmed | Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique |
| title_short | Detection of Clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique |
| title_sort | detection of clostridium difficile among diarrheic children using cultural and polymerase chain reaction technique |
| topic | Clostridium difficile diarrhea culture PCR |
| url | https://jidc.org/index.php/journal/article/view/17473 |
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