A universal method for the purification of C2H2 zinc finger arrays.
Zinc fingers (ZFs) are compact, modular, sequence-specific polynucleotide-binding domains uniquely suited for use as DNA probes and for the targeted delivery of effector domains for purposes such as gene regulation and editing. Despite recent advances in both the design and application of ZF-contain...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0318295 |
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| author | Jingchang Liang Maia Azubel Guanqiao Wang Yan Nie Roger D Kornberg Andrew J Beel Pierre-Jean Mattei |
| author_facet | Jingchang Liang Maia Azubel Guanqiao Wang Yan Nie Roger D Kornberg Andrew J Beel Pierre-Jean Mattei |
| author_sort | Jingchang Liang |
| collection | DOAJ |
| description | Zinc fingers (ZFs) are compact, modular, sequence-specific polynucleotide-binding domains uniquely suited for use as DNA probes and for the targeted delivery of effector domains for purposes such as gene regulation and editing. Despite recent advances in both the design and application of ZF-containing proteins, there is still a lack of a general method for their expression and purification. Here we describe a simple method, involving two chromatographic steps, for the production of homogeneous, functional ZF proteins in high yield (one milligram per liter of bacterial culture), and we demonstrate the generality of this method by applying it to a diverse set of eight C2H2-type ZF proteins. By incorporating a surface-exposed terminal cysteine residue that enables site-specific conjugation with maleimide-activated fluorophores, we confirm the suitability of these probes for in situ labeling of specific DNA sequences in human cells. |
| format | Article |
| id | doaj-art-be06136a424a4154bc4533f96c68eac6 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-be06136a424a4154bc4533f96c68eac62025-02-12T05:31:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031829510.1371/journal.pone.0318295A universal method for the purification of C2H2 zinc finger arrays.Jingchang LiangMaia AzubelGuanqiao WangYan NieRoger D KornbergAndrew J BeelPierre-Jean MatteiZinc fingers (ZFs) are compact, modular, sequence-specific polynucleotide-binding domains uniquely suited for use as DNA probes and for the targeted delivery of effector domains for purposes such as gene regulation and editing. Despite recent advances in both the design and application of ZF-containing proteins, there is still a lack of a general method for their expression and purification. Here we describe a simple method, involving two chromatographic steps, for the production of homogeneous, functional ZF proteins in high yield (one milligram per liter of bacterial culture), and we demonstrate the generality of this method by applying it to a diverse set of eight C2H2-type ZF proteins. By incorporating a surface-exposed terminal cysteine residue that enables site-specific conjugation with maleimide-activated fluorophores, we confirm the suitability of these probes for in situ labeling of specific DNA sequences in human cells.https://doi.org/10.1371/journal.pone.0318295 |
| spellingShingle | Jingchang Liang Maia Azubel Guanqiao Wang Yan Nie Roger D Kornberg Andrew J Beel Pierre-Jean Mattei A universal method for the purification of C2H2 zinc finger arrays. PLoS ONE |
| title | A universal method for the purification of C2H2 zinc finger arrays. |
| title_full | A universal method for the purification of C2H2 zinc finger arrays. |
| title_fullStr | A universal method for the purification of C2H2 zinc finger arrays. |
| title_full_unstemmed | A universal method for the purification of C2H2 zinc finger arrays. |
| title_short | A universal method for the purification of C2H2 zinc finger arrays. |
| title_sort | universal method for the purification of c2h2 zinc finger arrays |
| url | https://doi.org/10.1371/journal.pone.0318295 |
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