Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years
Abstract Hemorrhagic disease caused by bluetongue virus (BTV) significantly impacts Florida’s deer farming industry each year. In 2022, we investigated mortality events in white-tailed deer on two separate farms. Necropsies were performed on affected animals, and tissues were subjected to RT-qPCR sc...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2025-07-01
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| Series: | Discover Viruses |
| Online Access: | https://doi.org/10.1007/s44370-025-00023-y |
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| author | Pacharapong Khrongsee Pedro H. O. Viadanna Kristen N. Wilson Tracey L. Moquin Juan M. Campos Krauer John A. Lednicky Samantha M. Wisely Kuttichantran Subramaniam |
| author_facet | Pacharapong Khrongsee Pedro H. O. Viadanna Kristen N. Wilson Tracey L. Moquin Juan M. Campos Krauer John A. Lednicky Samantha M. Wisely Kuttichantran Subramaniam |
| author_sort | Pacharapong Khrongsee |
| collection | DOAJ |
| description | Abstract Hemorrhagic disease caused by bluetongue virus (BTV) significantly impacts Florida’s deer farming industry each year. In 2022, we investigated mortality events in white-tailed deer on two separate farms. Necropsies were performed on affected animals, and tissues were subjected to RT-qPCR screening and molecular diagnostics, confirming BTV infection. Virus isolation in C6/36 cell culture and whole-genome sequencing identified BTV serotype 5 (BTV-5) infection in both fawns—the first cases reported in Florida since 2003. Our analyses further reveal that key serotype-defining genome segments (2 and 6) remained conserved, while other segments exhibited evidence of reassortment with co-circulating BTV strains in the region. This suggests that BTV-5 may have persisted undetected at low levels, undergoing genetic exchange with co-circulating strains. These findings highlight the need for improved surveillance programs to monitor genetic changes and mitigate impacts on deer and other ruminants in Florida. |
| format | Article |
| id | doaj-art-be03fe9643cd4dfa91fce635f927f27d |
| institution | Kabale University |
| issn | 3005-0960 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Discover Viruses |
| spelling | doaj-art-be03fe9643cd4dfa91fce635f927f27d2025-08-20T03:45:56ZengSpringer NatureDiscover Viruses3005-09602025-07-01211910.1007/s44370-025-00023-yGenome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 yearsPacharapong Khrongsee0Pedro H. O. Viadanna1Kristen N. Wilson2Tracey L. Moquin3Juan M. Campos Krauer4John A. Lednicky5Samantha M. Wisely6Kuttichantran Subramaniam7Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of FloridaDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of FloridaEmerging Pathogens Institute, University of FloridaEmerging Pathogens Institute, University of FloridaDepartment of Wildlife Ecology and Conservation, University of FloridaEmerging Pathogens Institute, University of FloridaEmerging Pathogens Institute, University of FloridaDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of FloridaAbstract Hemorrhagic disease caused by bluetongue virus (BTV) significantly impacts Florida’s deer farming industry each year. In 2022, we investigated mortality events in white-tailed deer on two separate farms. Necropsies were performed on affected animals, and tissues were subjected to RT-qPCR screening and molecular diagnostics, confirming BTV infection. Virus isolation in C6/36 cell culture and whole-genome sequencing identified BTV serotype 5 (BTV-5) infection in both fawns—the first cases reported in Florida since 2003. Our analyses further reveal that key serotype-defining genome segments (2 and 6) remained conserved, while other segments exhibited evidence of reassortment with co-circulating BTV strains in the region. This suggests that BTV-5 may have persisted undetected at low levels, undergoing genetic exchange with co-circulating strains. These findings highlight the need for improved surveillance programs to monitor genetic changes and mitigate impacts on deer and other ruminants in Florida.https://doi.org/10.1007/s44370-025-00023-y |
| spellingShingle | Pacharapong Khrongsee Pedro H. O. Viadanna Kristen N. Wilson Tracey L. Moquin Juan M. Campos Krauer John A. Lednicky Samantha M. Wisely Kuttichantran Subramaniam Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years Discover Viruses |
| title | Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years |
| title_full | Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years |
| title_fullStr | Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years |
| title_full_unstemmed | Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years |
| title_short | Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years |
| title_sort | genome characterization of two bluetongue virus serotype 5 strains isolated from white tailed deer first detected cases in florida after 19 years |
| url | https://doi.org/10.1007/s44370-025-00023-y |
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