Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature
Lacquer trees (<i>Toxicodendron vernicifluum</i>), economically vital, face high-temperature stress in summer. Transcriptomic, proteomic, and metabolomic analyses were employed to investigate the mechanisms by which lacquer trees respond to high temperatures. High-temperature treatment l...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/13/11/876 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145075262128128 |
|---|---|
| author | Guoqing Bai Ruiwen Ding Qizhen Su Xiaomin Ge Shasha Li Huiying Shang Aiguo Zhao Chen Chen |
| author_facet | Guoqing Bai Ruiwen Ding Qizhen Su Xiaomin Ge Shasha Li Huiying Shang Aiguo Zhao Chen Chen |
| author_sort | Guoqing Bai |
| collection | DOAJ |
| description | Lacquer trees (<i>Toxicodendron vernicifluum</i>), economically vital, face high-temperature stress in summer. Transcriptomic, proteomic, and metabolomic analyses were employed to investigate the mechanisms by which lacquer trees respond to high temperatures. High-temperature treatment led to notable metabolite changes with 224 upregulated and 69 downregulated. Indole-3-acetic acid remained stable while abscisic acid decreased, with increases in jasmonic acid and jasmonoyl-L-isoleucine indicating complex hormonal responses. JAR1 and ABA 8′-hydroxylase encoding genes were upregulated. The rise in JAs boosted the alkaloid content and activated nitrogen transport. High temperatures also increased specific amino acids and upregulated aminotransferase and protease-encoding genes. Metabolomic analysis showed elevated flavonoid glycosides and the upregulation of glycosyltransferase genes. WPCNA found 35 protein modules involved in secondary metabolite biosynthesis, protein phosphorylation, and signal transduction. Protein–protein interaction analysis revealed MYC6’s link with flavonoid biosynthesis, indicating its role in promoting flavonoids. |
| format | Article |
| id | doaj-art-be00664b5c094cccb005ed0d53aa0ccc |
| institution | OA Journals |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-be00664b5c094cccb005ed0d53aa0ccc2025-08-20T02:28:11ZengMDPI AGBiology2079-77372024-10-01131187610.3390/biology13110876Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High TemperatureGuoqing Bai0Ruiwen Ding1Qizhen Su2Xiaomin Ge3Shasha Li4Huiying Shang5Aiguo Zhao6Chen Chen7Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, ChinaEast China Academy of Inventory and Planning of NFGA, Hangzhou 310019, ChinaQinling National Botanical Garden, Xi’an 710061, ChinaShaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, ChinaShaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, ChinaShaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, ChinaCollege of Forestry, Northwest A&F University, Yangling 712100, ChinaShaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, ChinaLacquer trees (<i>Toxicodendron vernicifluum</i>), economically vital, face high-temperature stress in summer. Transcriptomic, proteomic, and metabolomic analyses were employed to investigate the mechanisms by which lacquer trees respond to high temperatures. High-temperature treatment led to notable metabolite changes with 224 upregulated and 69 downregulated. Indole-3-acetic acid remained stable while abscisic acid decreased, with increases in jasmonic acid and jasmonoyl-L-isoleucine indicating complex hormonal responses. JAR1 and ABA 8′-hydroxylase encoding genes were upregulated. The rise in JAs boosted the alkaloid content and activated nitrogen transport. High temperatures also increased specific amino acids and upregulated aminotransferase and protease-encoding genes. Metabolomic analysis showed elevated flavonoid glycosides and the upregulation of glycosyltransferase genes. WPCNA found 35 protein modules involved in secondary metabolite biosynthesis, protein phosphorylation, and signal transduction. Protein–protein interaction analysis revealed MYC6’s link with flavonoid biosynthesis, indicating its role in promoting flavonoids.https://www.mdpi.com/2079-7737/13/11/876<i>Toxicodendron vernicifluum</i>high temperaturealkaloidflavonoid |
| spellingShingle | Guoqing Bai Ruiwen Ding Qizhen Su Xiaomin Ge Shasha Li Huiying Shang Aiguo Zhao Chen Chen Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature Biology <i>Toxicodendron vernicifluum</i> high temperature alkaloid flavonoid |
| title | Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature |
| title_full | Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature |
| title_fullStr | Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature |
| title_full_unstemmed | Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature |
| title_short | Multiple Omics Analyses Reveal Activation of Nitrogen Metabolism and Flavonoid Glycosylation in <i>Toxicodendron vernicifluum</i> Under High Temperature |
| title_sort | multiple omics analyses reveal activation of nitrogen metabolism and flavonoid glycosylation in i toxicodendron vernicifluum i under high temperature |
| topic | <i>Toxicodendron vernicifluum</i> high temperature alkaloid flavonoid |
| url | https://www.mdpi.com/2079-7737/13/11/876 |
| work_keys_str_mv | AT guoqingbai multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT ruiwending multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT qizhensu multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT xiaominge multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT shashali multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT huiyingshang multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT aiguozhao multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature AT chenchen multipleomicsanalysesrevealactivationofnitrogenmetabolismandflavonoidglycosylationinitoxicodendronvernicifluumiunderhightemperature |