Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research
Single-cell adhesion assays can be divided into studies on attachment and detachment events, and several methods that enable the characterization of both processes have been established in the past. Due to their low invasiveness, label-free principles, and contactless operation, optical methods are...
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MDPI AG
2025-06-01
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| author | Kamila Duś-Szachniewicz Sławomir Drobczyński |
| author_facet | Kamila Duś-Szachniewicz Sławomir Drobczyński |
| author_sort | Kamila Duś-Szachniewicz |
| collection | DOAJ |
| description | Single-cell adhesion assays can be divided into studies on attachment and detachment events, and several methods that enable the characterization of both processes have been established in the past. Due to their low invasiveness, label-free principles, and contactless operation, optical methods are especially beneficial for this purpose. Historically, optical tweezers (OTs) have been used to explore single-cell detachment events, allowing for the precise determination of minute physical forces. However, it has been noted that OTs can also be used to study single-cell attachment dynamics, including the evaluation of minimum cell-to-cell contact times necessary to establish a stable adhesive bond. Here, we provide a step-by-step protocol to effectively evaluate minute changes in the adhesion of single leukemia–lymphoma cells using optical tweezers with low laser intensities. This serves as a valuable in vitro model to determine the effects of physical and chemical factors on the adhesive properties of leukemia–lymphoma (LL) cells. |
| format | Article |
| id | doaj-art-bdf11b4ee4a8433bb86bf3b57d2e1d10 |
| institution | Kabale University |
| issn | 2409-9279 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj-art-bdf11b4ee4a8433bb86bf3b57d2e1d102025-08-20T03:27:32ZengMDPI AGMethods and Protocols2409-92792025-06-01835910.3390/mps8030059Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma ResearchKamila Duś-Szachniewicz0Sławomir Drobczyński1Department of Clinical and Experimental Pathology, Institute of General and Experimental Pathology, Wrocław Medical University, 50-368 Wrocław, PolandDepartment of Optics and Photonics, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, 50-370 Wrocław, PolandSingle-cell adhesion assays can be divided into studies on attachment and detachment events, and several methods that enable the characterization of both processes have been established in the past. Due to their low invasiveness, label-free principles, and contactless operation, optical methods are especially beneficial for this purpose. Historically, optical tweezers (OTs) have been used to explore single-cell detachment events, allowing for the precise determination of minute physical forces. However, it has been noted that OTs can also be used to study single-cell attachment dynamics, including the evaluation of minimum cell-to-cell contact times necessary to establish a stable adhesive bond. Here, we provide a step-by-step protocol to effectively evaluate minute changes in the adhesion of single leukemia–lymphoma cells using optical tweezers with low laser intensities. This serves as a valuable in vitro model to determine the effects of physical and chemical factors on the adhesive properties of leukemia–lymphoma (LL) cells.https://www.mdpi.com/2409-9279/8/3/59optical tweezersleukemia–lymphoma (LL) cell linesminimum cell-to-cell adhesion timesingle cell adhesion assayhematological malignancies |
| spellingShingle | Kamila Duś-Szachniewicz Sławomir Drobczyński Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research Methods and Protocols optical tweezers leukemia–lymphoma (LL) cell lines minimum cell-to-cell adhesion time single cell adhesion assay hematological malignancies |
| title | Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research |
| title_full | Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research |
| title_fullStr | Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research |
| title_full_unstemmed | Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research |
| title_short | Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research |
| title_sort | determination of the minimum cell to cell adhesion time using optical tweezers in leukemia and lymphoma research |
| topic | optical tweezers leukemia–lymphoma (LL) cell lines minimum cell-to-cell adhesion time single cell adhesion assay hematological malignancies |
| url | https://www.mdpi.com/2409-9279/8/3/59 |
| work_keys_str_mv | AT kamiladusszachniewicz determinationoftheminimumcelltocelladhesiontimeusingopticaltweezersinleukemiaandlymphomaresearch AT sławomirdrobczynski determinationoftheminimumcelltocelladhesiontimeusingopticaltweezersinleukemiaandlymphomaresearch |