Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope
Introduction: Invasive Candida infections have recently shown a significant increase in prevalence and are associated with high mortality rates. Initiating early antifungal treatment in patients with candidemia is vital. The aim of our study was to compare the antifungal susceptibility results of a...
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The Journal of Infection in Developing Countries
2024-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/19253 |
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| author | Demet Gür Vural Gülşen Çetin Kemal Bilgin Yeliz Tanrıverdi Çaycı Asuman Birinci |
| author_facet | Demet Gür Vural Gülşen Çetin Kemal Bilgin Yeliz Tanrıverdi Çaycı Asuman Birinci |
| author_sort | Demet Gür Vural |
| collection | DOAJ |
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Introduction: Invasive Candida infections have recently shown a significant increase in prevalence and are associated with high mortality rates. Initiating early antifungal treatment in patients with candidemia is vital. The aim of our study was to compare the antifungal susceptibility results of a new method called Flat Plate Method modified from reference "Clinical and Laboratory Standards Institute (CLSI) microdilution method by us with Sensitititre Yeast One colorimetric method and the reference CLSI method.
Methodology: We tested 100 Candida isolates from blood cultures. We followed the CLSI M27-A3 (reference method for broth dilution antifungal susceptibility testing of yeasts; third edition) guidelines for testing in vitro susceptibility to amphotericin B. In the Flat Plate method, 96-well plates were used for evaluation with an inverted microscope. Minimum inhibitory concentration (MIC) values in the SYO method were measured following the manufacturer’s instructions. The MIC values obtained by all three methods were considered compatible if they were within ± 2 dilution limits.
Results: The SYO method detected C. albicans and C. glabrata with 100% essential agreement, whereas there was 96.29% essential agreement in the case of C. parapsilosis. In the Flat Plate method, the essential agreement with amphotericin B was 91.42%, for C. albicans isolates and 89.47%.for C. glabrata strains.
Conclusions: When determining early antifungal susceptibility using the Flat Plate method, the results are obtained quickly, with high accuracy, and without incurring additional costs. However, there is a need for comprehensive studies comparing different antifungals.
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| format | Article |
| id | doaj-art-bdeda2c4ffee46d6957146235cc491e7 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-bdeda2c4ffee46d6957146235cc491e72025-08-20T02:57:17ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-02-01180210.3855/jidc.19253Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscopeDemet Gür Vural0Gülşen Çetin1Kemal Bilgin2Yeliz Tanrıverdi Çaycı3Asuman Birinci4Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey Introduction: Invasive Candida infections have recently shown a significant increase in prevalence and are associated with high mortality rates. Initiating early antifungal treatment in patients with candidemia is vital. The aim of our study was to compare the antifungal susceptibility results of a new method called Flat Plate Method modified from reference "Clinical and Laboratory Standards Institute (CLSI) microdilution method by us with Sensitititre Yeast One colorimetric method and the reference CLSI method. Methodology: We tested 100 Candida isolates from blood cultures. We followed the CLSI M27-A3 (reference method for broth dilution antifungal susceptibility testing of yeasts; third edition) guidelines for testing in vitro susceptibility to amphotericin B. In the Flat Plate method, 96-well plates were used for evaluation with an inverted microscope. Minimum inhibitory concentration (MIC) values in the SYO method were measured following the manufacturer’s instructions. The MIC values obtained by all three methods were considered compatible if they were within ± 2 dilution limits. Results: The SYO method detected C. albicans and C. glabrata with 100% essential agreement, whereas there was 96.29% essential agreement in the case of C. parapsilosis. In the Flat Plate method, the essential agreement with amphotericin B was 91.42%, for C. albicans isolates and 89.47%.for C. glabrata strains. Conclusions: When determining early antifungal susceptibility using the Flat Plate method, the results are obtained quickly, with high accuracy, and without incurring additional costs. However, there is a need for comprehensive studies comparing different antifungals. https://jidc.org/index.php/journal/article/view/19253Candidaamphotericin Bsusceptibility |
| spellingShingle | Demet Gür Vural Gülşen Çetin Kemal Bilgin Yeliz Tanrıverdi Çaycı Asuman Birinci Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope Journal of Infection in Developing Countries Candida amphotericin B susceptibility |
| title | Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope |
| title_full | Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope |
| title_fullStr | Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope |
| title_full_unstemmed | Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope |
| title_short | Determination of amphotericin B antifungal susceptibility in Candida strains using an inverted microscope |
| title_sort | determination of amphotericin b antifungal susceptibility in candida strains using an inverted microscope |
| topic | Candida amphotericin B susceptibility |
| url | https://jidc.org/index.php/journal/article/view/19253 |
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