Size-dependent interactions between calciprotein particles and vascular endothelium
The underlying mechanisms governing the interactions between nanoparticles and vascular endothelial barrier remain largely unexplored, which is crucial for the optimal design of nanoparticles for clinical applications. In this study, the size-dependent interactions between calciprotein particles (CP...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425001577 |
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| author | Zeping Zhang Xinyue Wang Caihao Huang Meixia Wang Wei Cui Liang Hao Rui Yang Hong-hui Wang Xing Zhang |
| author_facet | Zeping Zhang Xinyue Wang Caihao Huang Meixia Wang Wei Cui Liang Hao Rui Yang Hong-hui Wang Xing Zhang |
| author_sort | Zeping Zhang |
| collection | DOAJ |
| description | The underlying mechanisms governing the interactions between nanoparticles and vascular endothelial barrier remain largely unexplored, which is crucial for the optimal design of nanoparticles for clinical applications. In this study, the size-dependent interactions between calciprotein particles (CPPs) and endothelial cells (ECs) were investigated using a rat model of chronic kidney disease (CKD) induced by 5/6 nephrectomy. Two primary types of CPP1 were studied: small-sized CPP1 (S-CPP1, <50 nm) and larger CPP1 (L-CPP1, <100 nm), detected three and five weeks post-surgery, respectively. By adjusting the amounts of Ca2+, HPO42− and H2PO4− ions in Dulbecco's Modified Eagle Medium supplemented with 10 % (V/V) fetal bovine serum and 1 % (V/V) Pen-Strep solution, S-CPP1 (<50 nm) with an elliptical shape, L-CPP1 (50–100 nm), and secondary CPPs (CPP2, >100 nm) with a needle-like crystalline structure, resembling endogenous CPPs, were synthesized. The results showed that S-CPP1 significantly increased endothelial permeability at concentrations of 445 μg/mL and 890 μg/mL, thereby disrupting the integrity of the endothelial barrier formed by a confluent monolayer of ECs. Immunofluorescence analysis revealed that L-CPP1 was internalized by ECs via endocytosis, while S-CPP1 disrupted VE-cadherin junctions, leading to irregular cell morphology and widened intercellular gaps. These structural changes likely contribute to medial calcification as CPPs accumulate within the circulatory system. In conclusion, the findings underscore that the interaction between CPPs and the vascular endothelium is strongly size-dependent, with significant implications for vascular system health and the design of nanoparticle-based therapies. |
| format | Article |
| id | doaj-art-bdd8e1027507456699b2aefd3b804c6b |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-bdd8e1027507456699b2aefd3b804c6b2025-08-20T03:13:18ZengElsevierMaterials Today Bio2590-00642025-04-013110159910.1016/j.mtbio.2025.101599Size-dependent interactions between calciprotein particles and vascular endotheliumZeping Zhang0Xinyue Wang1Caihao Huang2Meixia Wang3Wei Cui4Liang Hao5Rui Yang6Hong-hui Wang7Xing Zhang8Institute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China; School of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, ChinaInstitute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China; School of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, ChinaInstitute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China; School of Materials Science and Engineering, Dalian University of Technology, Dalian, Liaoning 116024, ChinaState Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, ChinaInstitute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China; School of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, ChinaSchool of Forensic Medicine, China Medical University, Shenyang, Liaoning 110026, ChinaInstitute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China; School of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, ChinaState Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, China; Corresponding author.Institute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China; School of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China; Corresponding author. Institute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China.The underlying mechanisms governing the interactions between nanoparticles and vascular endothelial barrier remain largely unexplored, which is crucial for the optimal design of nanoparticles for clinical applications. In this study, the size-dependent interactions between calciprotein particles (CPPs) and endothelial cells (ECs) were investigated using a rat model of chronic kidney disease (CKD) induced by 5/6 nephrectomy. Two primary types of CPP1 were studied: small-sized CPP1 (S-CPP1, <50 nm) and larger CPP1 (L-CPP1, <100 nm), detected three and five weeks post-surgery, respectively. By adjusting the amounts of Ca2+, HPO42− and H2PO4− ions in Dulbecco's Modified Eagle Medium supplemented with 10 % (V/V) fetal bovine serum and 1 % (V/V) Pen-Strep solution, S-CPP1 (<50 nm) with an elliptical shape, L-CPP1 (50–100 nm), and secondary CPPs (CPP2, >100 nm) with a needle-like crystalline structure, resembling endogenous CPPs, were synthesized. The results showed that S-CPP1 significantly increased endothelial permeability at concentrations of 445 μg/mL and 890 μg/mL, thereby disrupting the integrity of the endothelial barrier formed by a confluent monolayer of ECs. Immunofluorescence analysis revealed that L-CPP1 was internalized by ECs via endocytosis, while S-CPP1 disrupted VE-cadherin junctions, leading to irregular cell morphology and widened intercellular gaps. These structural changes likely contribute to medial calcification as CPPs accumulate within the circulatory system. In conclusion, the findings underscore that the interaction between CPPs and the vascular endothelium is strongly size-dependent, with significant implications for vascular system health and the design of nanoparticle-based therapies.http://www.sciencedirect.com/science/article/pii/S2590006425001577EndotheliumCalciprotein nanoparticlesChronic kidney diseaseBiomimetic synthesis |
| spellingShingle | Zeping Zhang Xinyue Wang Caihao Huang Meixia Wang Wei Cui Liang Hao Rui Yang Hong-hui Wang Xing Zhang Size-dependent interactions between calciprotein particles and vascular endothelium Materials Today Bio Endothelium Calciprotein nanoparticles Chronic kidney disease Biomimetic synthesis |
| title | Size-dependent interactions between calciprotein particles and vascular endothelium |
| title_full | Size-dependent interactions between calciprotein particles and vascular endothelium |
| title_fullStr | Size-dependent interactions between calciprotein particles and vascular endothelium |
| title_full_unstemmed | Size-dependent interactions between calciprotein particles and vascular endothelium |
| title_short | Size-dependent interactions between calciprotein particles and vascular endothelium |
| title_sort | size dependent interactions between calciprotein particles and vascular endothelium |
| topic | Endothelium Calciprotein nanoparticles Chronic kidney disease Biomimetic synthesis |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425001577 |
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