Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells

This study aimed to construct an eukaryotic expression vector, pEGFP-N1-MIC-1, for overexpressing the mouse macrophage inhibitory cytokine-1 (MIC-1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC-1 gene expression and assess its impact on macrophage phenotype...

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Bibliographic Details
Main Authors: HuiPeng Zhang, Zhongyu Qin, ShuaiShuai Shi, YunFei Li, Yang Song, YiQiang Zhang
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2024/2165242
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