Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells
This study aimed to construct an eukaryotic expression vector, pEGFP-N1-MIC-1, for overexpressing the mouse macrophage inhibitory cytokine-1 (MIC-1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC-1 gene expression and assess its impact on macrophage phenotype...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2024/2165242 |
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| _version_ | 1850178998319972352 |
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| author | HuiPeng Zhang Zhongyu Qin ShuaiShuai Shi YunFei Li Yang Song YiQiang Zhang |
| author_facet | HuiPeng Zhang Zhongyu Qin ShuaiShuai Shi YunFei Li Yang Song YiQiang Zhang |
| author_sort | HuiPeng Zhang |
| collection | DOAJ |
| description | This study aimed to construct an eukaryotic expression vector, pEGFP-N1-MIC-1, for overexpressing the mouse macrophage inhibitory cytokine-1 (MIC-1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC-1 gene expression and assess its impact on macrophage phenotype conversion. Enzyme digestion and DNA sequencing confirmed the successful construction of the pEGFP-N1-MIC-1 vector. The transfected MFC cells exhibited a significant increase in MIC-1 protein expression levels. Furthermore, transfection with pEGFP-N1-MIC-1 increased the migration and colony formation capabilities of MFC cells. These results may contribute to future research and the development of therapeutic interventions targeting MIC-1 in macrophages, particularly in the context of gastric cancer. |
| format | Article |
| id | doaj-art-bdd37d0307104effbd45c86d8eeead08 |
| institution | OA Journals |
| issn | 2210-7185 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-bdd37d0307104effbd45c86d8eeead082025-08-20T02:18:36ZengWileyAnalytical Cellular Pathology2210-71852024-01-01202410.1155/2024/2165242Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer CellsHuiPeng Zhang0Zhongyu Qin1ShuaiShuai Shi2YunFei Li3Yang Song4YiQiang Zhang5Department of General SurgeryDepartment of Basic MedicineDepartment of NephrologyInstitute of First ClinicalLaboratory Animal Center (LAC)Department of Basic MedicineThis study aimed to construct an eukaryotic expression vector, pEGFP-N1-MIC-1, for overexpressing the mouse macrophage inhibitory cytokine-1 (MIC-1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC-1 gene expression and assess its impact on macrophage phenotype conversion. Enzyme digestion and DNA sequencing confirmed the successful construction of the pEGFP-N1-MIC-1 vector. The transfected MFC cells exhibited a significant increase in MIC-1 protein expression levels. Furthermore, transfection with pEGFP-N1-MIC-1 increased the migration and colony formation capabilities of MFC cells. These results may contribute to future research and the development of therapeutic interventions targeting MIC-1 in macrophages, particularly in the context of gastric cancer.http://dx.doi.org/10.1155/2024/2165242 |
| spellingShingle | HuiPeng Zhang Zhongyu Qin ShuaiShuai Shi YunFei Li Yang Song YiQiang Zhang Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells Analytical Cellular Pathology |
| title | Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells |
| title_full | Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells |
| title_fullStr | Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells |
| title_full_unstemmed | Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells |
| title_short | Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells |
| title_sort | construction and identification of eukaryotic expression vector pegfp n1 mic 1 for mouse mic 1 gene and its effect on gastric cancer cells |
| url | http://dx.doi.org/10.1155/2024/2165242 |
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