Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells.
Two key events, namely adhesion and invasion, are pivotal to the occurrence of metastasis. Importantly, the 37 kDa/67 kDa laminin receptor (LRP/LR) has been implicated in enhancing these two events thus facilitating cancer progression. In the current study, the role of LRP/LR in the adhesion and inv...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0096268&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850192014144962560 |
|---|---|
| author | Carryn Chetty Thandokuhle Khumalo Bianca Da Costa Dias Uwe Reusch Stefan Knackmuss Melvyn Little Stefan F T Weiss |
| author_facet | Carryn Chetty Thandokuhle Khumalo Bianca Da Costa Dias Uwe Reusch Stefan Knackmuss Melvyn Little Stefan F T Weiss |
| author_sort | Carryn Chetty |
| collection | DOAJ |
| description | Two key events, namely adhesion and invasion, are pivotal to the occurrence of metastasis. Importantly, the 37 kDa/67 kDa laminin receptor (LRP/LR) has been implicated in enhancing these two events thus facilitating cancer progression. In the current study, the role of LRP/LR in the adhesion and invasion of liver cancer (HUH-7) and leukaemia (K562) cells was investigated. Flow cytometry revealed that the HUH-7 cells displayed significantly higher cell surface LRP/LR levels compared to the poorly-invasive breast cancer (MCF-7) control cells, whilst the K562 cells displayed significantly lower cell surface LRP/LR levels in comparison to the MCF-7 control cells. However, Western blotting and densitometric analysis revealed that all three tumorigenic cell lines did not differ significantly with regards to total LRP/LR levels. Furthermore, treatment of liver cancer cells with anti-LRP/LR specific antibody IgG1-iS18 (0.2 mg/ml) significantly reduced the adhesive potential of cells to laminin-1 and the invasive potential of cells through the ECM-like Matrigel, whilst leukaemia cells showed no significant differences in both instances. Additionally, Pearson's correlation coefficients suggested direct proportionality between cell surface LRP/LR levels and the adhesive and invasive potential of liver cancer and leukaemia cells. These findings suggest the potential use of anti-LRP/LR specific antibody IgG1-iS18 as an alternative therapeutic tool for metastatic liver cancer through impediment of the LRP/LR- laminin-1 interaction. |
| format | Article |
| id | doaj-art-bdbf3ea3158c47489cd2da5a661970a7 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-bdbf3ea3158c47489cd2da5a661970a72025-08-20T02:14:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9626810.1371/journal.pone.0096268Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells.Carryn ChettyThandokuhle KhumaloBianca Da Costa DiasUwe ReuschStefan KnackmussMelvyn LittleStefan F T WeissTwo key events, namely adhesion and invasion, are pivotal to the occurrence of metastasis. Importantly, the 37 kDa/67 kDa laminin receptor (LRP/LR) has been implicated in enhancing these two events thus facilitating cancer progression. In the current study, the role of LRP/LR in the adhesion and invasion of liver cancer (HUH-7) and leukaemia (K562) cells was investigated. Flow cytometry revealed that the HUH-7 cells displayed significantly higher cell surface LRP/LR levels compared to the poorly-invasive breast cancer (MCF-7) control cells, whilst the K562 cells displayed significantly lower cell surface LRP/LR levels in comparison to the MCF-7 control cells. However, Western blotting and densitometric analysis revealed that all three tumorigenic cell lines did not differ significantly with regards to total LRP/LR levels. Furthermore, treatment of liver cancer cells with anti-LRP/LR specific antibody IgG1-iS18 (0.2 mg/ml) significantly reduced the adhesive potential of cells to laminin-1 and the invasive potential of cells through the ECM-like Matrigel, whilst leukaemia cells showed no significant differences in both instances. Additionally, Pearson's correlation coefficients suggested direct proportionality between cell surface LRP/LR levels and the adhesive and invasive potential of liver cancer and leukaemia cells. These findings suggest the potential use of anti-LRP/LR specific antibody IgG1-iS18 as an alternative therapeutic tool for metastatic liver cancer through impediment of the LRP/LR- laminin-1 interaction.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0096268&type=printable |
| spellingShingle | Carryn Chetty Thandokuhle Khumalo Bianca Da Costa Dias Uwe Reusch Stefan Knackmuss Melvyn Little Stefan F T Weiss Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. PLoS ONE |
| title | Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. |
| title_full | Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. |
| title_fullStr | Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. |
| title_full_unstemmed | Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. |
| title_short | Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. |
| title_sort | anti lrp lr specific antibody igg1 is18 impedes adhesion and invasion of liver cancer cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0096268&type=printable |
| work_keys_str_mv | AT carrynchetty antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells AT thandokuhlekhumalo antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells AT biancadacostadias antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells AT uwereusch antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells AT stefanknackmuss antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells AT melvynlittle antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells AT stefanftweiss antilrplrspecificantibodyigg1is18impedesadhesionandinvasionoflivercancercells |