Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains
Xylanase produced from Trichoderma spp. can induce plant resistance, which is one of important biocontrol mechanisms of fungal Trichoderma. The effect of cultural condition on the producing xylanase by T. harzianum strains was investigated. Appropriate pH, temperature, concentration of surface activ...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2009-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.004 |
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| _version_ | 1849412852529496064 |
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| author | LIU Ya-li LIU Lu-ning ZHANG Jing-ze XU Tong |
| author_facet | LIU Ya-li LIU Lu-ning ZHANG Jing-ze XU Tong |
| author_sort | LIU Ya-li |
| collection | DOAJ |
| description | Xylanase produced from Trichoderma spp. can induce plant resistance, which is one of important biocontrol mechanisms of fungal Trichoderma. The effect of cultural condition on the producing xylanase by T. harzianum strains was investigated. Appropriate pH, temperature, concentration of surface active agent Tween 80, incubation period were optimized for enzyme production. The results show that the culture conditions of maximum enzyme activities produced from different T. harzianum strains were different in shaker flasks with semi-solid media. Maximum enzyme activities were at pH 5.0, 30 ℃, 0.7%(concentration of surface active agent Tween 80) and 72 h for NF<sub>9</sub>, pH 4.0, 27 ℃, 1.2% and 84 h for P<sub>1</sub>, pH 5.0, 27 ℃, 0.7% and 84 h for CT<sub>22</sub>, pH 4.0, 30 ℃, 0.7% and 96 h for TC<sub>3</sub>, respectively. |
| format | Article |
| id | doaj-art-bdb64600494244d2a0dbbd833f17fc4e |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2009-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-bdb64600494244d2a0dbbd833f17fc4e2025-08-20T03:34:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-03-013514114610.3785/j.issn.1008-9209.2009.02.00410089209Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strainsLIU Ya-liLIU Lu-ningZHANG Jing-zeXU TongXylanase produced from Trichoderma spp. can induce plant resistance, which is one of important biocontrol mechanisms of fungal Trichoderma. The effect of cultural condition on the producing xylanase by T. harzianum strains was investigated. Appropriate pH, temperature, concentration of surface active agent Tween 80, incubation period were optimized for enzyme production. The results show that the culture conditions of maximum enzyme activities produced from different T. harzianum strains were different in shaker flasks with semi-solid media. Maximum enzyme activities were at pH 5.0, 30 ℃, 0.7%(concentration of surface active agent Tween 80) and 72 h for NF<sub>9</sub>, pH 4.0, 27 ℃, 1.2% and 84 h for P<sub>1</sub>, pH 5.0, 27 ℃, 0.7% and 84 h for CT<sub>22</sub>, pH 4.0, 30 ℃, 0.7% and 96 h for TC<sub>3</sub>, respectively.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.004biocontrol agent<italic>Trichoderma harzianum</italic> strainsxylanaseculture conditions |
| spellingShingle | LIU Ya-li LIU Lu-ning ZHANG Jing-ze XU Tong Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains 浙江大学学报. 农业与生命科学版 biocontrol agent <italic>Trichoderma harzianum</italic> strains xylanase culture conditions |
| title | Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains |
| title_full | Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains |
| title_fullStr | Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains |
| title_full_unstemmed | Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains |
| title_short | Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains |
| title_sort | effect of culture conditions on xylanase activity produced from biocontrol agent trichoderma harzianum strains |
| topic | biocontrol agent <italic>Trichoderma harzianum</italic> strains xylanase culture conditions |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.004 |
| work_keys_str_mv | AT liuyali effectofcultureconditionsonxylanaseactivityproducedfrombiocontrolagenttrichodermaharzianumstrains AT liuluning effectofcultureconditionsonxylanaseactivityproducedfrombiocontrolagenttrichodermaharzianumstrains AT zhangjingze effectofcultureconditionsonxylanaseactivityproducedfrombiocontrolagenttrichodermaharzianumstrains AT xutong effectofcultureconditionsonxylanaseactivityproducedfrombiocontrolagenttrichodermaharzianumstrains |