Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems

The interaction of microorganisms and their enzyme activity is one of the key indicators for a comprehensive measurement of soil health. The aim of this study was to determine significant correlations between different soil microorganisms and enzyme activities of β-glucosidase, N-acetyl-glucosaminid...

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Main Authors: Maša Pintarič, Ana Štuhec, Eva Tratnik, Tomaž Langerholc
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/14/12/1562
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author Maša Pintarič
Ana Štuhec
Eva Tratnik
Tomaž Langerholc
author_facet Maša Pintarič
Ana Štuhec
Eva Tratnik
Tomaž Langerholc
author_sort Maša Pintarič
collection DOAJ
description The interaction of microorganisms and their enzyme activity is one of the key indicators for a comprehensive measurement of soil health. The aim of this study was to determine significant correlations between different soil microorganisms and enzyme activities of β-glucosidase, N-acetyl-glucosaminidase, urease, arylamidase, phosphatase, acid phosphatase, alkaline phosphatase, and arylsulfatase after supplementation with standard fertilizer, spent mushroom substrate and composed fertilizer in soils from conventional–integrated, organic and biodynamic farming. Samples were grouped according to the farming system and fertilization for all seasons. The biodynamic farm was the least affected by the different fertilizations, except for standard fertilization. Standard fertilizer caused negative correlations between the actinomycetes and the arylsulfatase in organic and biodynamic farms. The same fertilization affected the actinomycetes/phosphatase relationship differently, regardless of the basic soil structure. Actinomycetes correlated positively with acid phosphatase and urease in conventional–integrated and biodynamic farms after spent mushroom substrate, respectively. Arylamidase activity in relation to total microorganisms responded to fertilization with standard fertilizer and spent mushroom substrate independently of the basic soil structure. Fertilization can influence the soil microbe/enzyme relationships in different soils. Regardless of the basic soil structure, some of these relationships could be important indicators for further studies.
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spelling doaj-art-bdb52ffe8f4349de9fcbbd79582b97072025-08-20T02:53:44ZengMDPI AGLife2075-17292024-11-011412156210.3390/life14121562Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming SystemsMaša Pintarič0Ana Štuhec1Eva Tratnik2Tomaž Langerholc3Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, SloveniaDepartment of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, SloveniaDepartment of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, SloveniaDepartment of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, SloveniaThe interaction of microorganisms and their enzyme activity is one of the key indicators for a comprehensive measurement of soil health. The aim of this study was to determine significant correlations between different soil microorganisms and enzyme activities of β-glucosidase, N-acetyl-glucosaminidase, urease, arylamidase, phosphatase, acid phosphatase, alkaline phosphatase, and arylsulfatase after supplementation with standard fertilizer, spent mushroom substrate and composed fertilizer in soils from conventional–integrated, organic and biodynamic farming. Samples were grouped according to the farming system and fertilization for all seasons. The biodynamic farm was the least affected by the different fertilizations, except for standard fertilization. Standard fertilizer caused negative correlations between the actinomycetes and the arylsulfatase in organic and biodynamic farms. The same fertilization affected the actinomycetes/phosphatase relationship differently, regardless of the basic soil structure. Actinomycetes correlated positively with acid phosphatase and urease in conventional–integrated and biodynamic farms after spent mushroom substrate, respectively. Arylamidase activity in relation to total microorganisms responded to fertilization with standard fertilizer and spent mushroom substrate independently of the basic soil structure. Fertilization can influence the soil microbe/enzyme relationships in different soils. Regardless of the basic soil structure, some of these relationships could be important indicators for further studies.https://www.mdpi.com/2075-1729/14/12/1562agricultureconventional–integratedorganicbiodynamicfertilizationsoil microorganisms
spellingShingle Maša Pintarič
Ana Štuhec
Eva Tratnik
Tomaž Langerholc
Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems
Life
agriculture
conventional–integrated
organic
biodynamic
fertilization
soil microorganisms
title Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems
title_full Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems
title_fullStr Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems
title_full_unstemmed Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems
title_short Specific Fertilization Practices Reveal Important Insights into the Complex Interaction Between Microbes and Enzymes in Soils of Different Farming Systems
title_sort specific fertilization practices reveal important insights into the complex interaction between microbes and enzymes in soils of different farming systems
topic agriculture
conventional–integrated
organic
biodynamic
fertilization
soil microorganisms
url https://www.mdpi.com/2075-1729/14/12/1562
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AT anastuhec specificfertilizationpracticesrevealimportantinsightsintothecomplexinteractionbetweenmicrobesandenzymesinsoilsofdifferentfarmingsystems
AT evatratnik specificfertilizationpracticesrevealimportantinsightsintothecomplexinteractionbetweenmicrobesandenzymesinsoilsofdifferentfarmingsystems
AT tomazlangerholc specificfertilizationpracticesrevealimportantinsightsintothecomplexinteractionbetweenmicrobesandenzymesinsoilsofdifferentfarmingsystems