Evaluation of bone development and organs in rat fetuses exposed to tartrazine
Tartrazine finds widespread application in the realms of alimentation, pharmaceuticals, cosmetic formulations, and textile manufacturing. Tartrazine has a negative effect on human health such as hyperactivity, allergies and asthma in children. Substances such as tartrazine might effect the embryo in...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024174877 |
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| author | Osman Öztürk Sümeyye Uçar Züleyha Doğanyiğit Aslı Okan Oflamaz Evrim Suna Arıkan Şükrü Ateş Seher Yılmaz |
| author_facet | Osman Öztürk Sümeyye Uçar Züleyha Doğanyiğit Aslı Okan Oflamaz Evrim Suna Arıkan Şükrü Ateş Seher Yılmaz |
| author_sort | Osman Öztürk |
| collection | DOAJ |
| description | Tartrazine finds widespread application in the realms of alimentation, pharmaceuticals, cosmetic formulations, and textile manufacturing. Tartrazine has a negative effect on human health such as hyperactivity, allergies and asthma in children. Substances such as tartrazine might effect the embryo in a kind of aspects, containing physical or mental disorders, and a decrease in the child's intellectual memory. In this study, Sprague dawley female rats, 70–100 days old, weighing 250–300 g, with confirmed pregnancy, were divided into two groups of 5: control and tartrazine group. Rats were sacrificed on the 20th day and heart, lung, kidney and liver tissues were removed from the fetuses. The effect of tartrazine on fetal bone development was assessed by double skeletal staining, histological analysis on organs, and IL-6, IL-1β, TNF-α, and TRPM2 gene levels. It has been observed that tartrazine, which is frequently used as a food dye, damages important fetal tissues such as liver, kidney, lung and heart. A statistically meaningful reduce was observed in the total length, total area, bone length and bone area values of the limb bones in the tartrazine group compared to the control group (p < 0.05). It was observed that the mRNA levels of IL-6, IL-1β, TNF-α and TRPM2 genes in the livers of the fetuses changed compared to the control group (p < 0.05). In this study on the use of tartrazine during pregnancy, it was observed that both organs and bone development were damaged. More studies are needed on the effects of tartrazine. |
| format | Article |
| id | doaj-art-bdb3ac4ee8634aa28b42af8e27a67f9f |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-bdb3ac4ee8634aa28b42af8e27a67f9f2025-01-17T04:51:20ZengElsevierHeliyon2405-84402025-01-01111e41456Evaluation of bone development and organs in rat fetuses exposed to tartrazineOsman Öztürk0Sümeyye Uçar1Züleyha Doğanyiğit2Aslı Okan Oflamaz3Evrim Suna Arıkan4Şükrü Ateş5Seher Yılmaz6Yozgat Bozok University, Faculty of Medicine, Department of Pediatric Health and Diseases, Yozgat, TurkeyErciyes University, Faculty of Medicine, Department of Anatomy, Kayseri, TurkeyYozgat Bozok University, Faculty of Medicine, Department of Histology and Embryology, Yozgat, TurkeyYozgat Bozok University, Faculty of Medicine, Department of Histology and Embryology, Yozgat, TurkeyAfyonkarahisar Health Sciences University, Faculty of Medicine, Department of Medical Biology, Afyonkarahisar, TurkeyYozgat Bozok University, Faculty of Medicine, Department of Anatomy, Yozgat, TurkeyYozgat Bozok University, Faculty of Medicine, Department of Anatomy, Yozgat, Turkey; Corresponding author.Tartrazine finds widespread application in the realms of alimentation, pharmaceuticals, cosmetic formulations, and textile manufacturing. Tartrazine has a negative effect on human health such as hyperactivity, allergies and asthma in children. Substances such as tartrazine might effect the embryo in a kind of aspects, containing physical or mental disorders, and a decrease in the child's intellectual memory. In this study, Sprague dawley female rats, 70–100 days old, weighing 250–300 g, with confirmed pregnancy, were divided into two groups of 5: control and tartrazine group. Rats were sacrificed on the 20th day and heart, lung, kidney and liver tissues were removed from the fetuses. The effect of tartrazine on fetal bone development was assessed by double skeletal staining, histological analysis on organs, and IL-6, IL-1β, TNF-α, and TRPM2 gene levels. It has been observed that tartrazine, which is frequently used as a food dye, damages important fetal tissues such as liver, kidney, lung and heart. A statistically meaningful reduce was observed in the total length, total area, bone length and bone area values of the limb bones in the tartrazine group compared to the control group (p < 0.05). It was observed that the mRNA levels of IL-6, IL-1β, TNF-α and TRPM2 genes in the livers of the fetuses changed compared to the control group (p < 0.05). In this study on the use of tartrazine during pregnancy, it was observed that both organs and bone development were damaged. More studies are needed on the effects of tartrazine.http://www.sciencedirect.com/science/article/pii/S2405844024174877TartrazineTeratogenic effectDouble skeleton stainingFood dyeRat fetuses |
| spellingShingle | Osman Öztürk Sümeyye Uçar Züleyha Doğanyiğit Aslı Okan Oflamaz Evrim Suna Arıkan Şükrü Ateş Seher Yılmaz Evaluation of bone development and organs in rat fetuses exposed to tartrazine Heliyon Tartrazine Teratogenic effect Double skeleton staining Food dye Rat fetuses |
| title | Evaluation of bone development and organs in rat fetuses exposed to tartrazine |
| title_full | Evaluation of bone development and organs in rat fetuses exposed to tartrazine |
| title_fullStr | Evaluation of bone development and organs in rat fetuses exposed to tartrazine |
| title_full_unstemmed | Evaluation of bone development and organs in rat fetuses exposed to tartrazine |
| title_short | Evaluation of bone development and organs in rat fetuses exposed to tartrazine |
| title_sort | evaluation of bone development and organs in rat fetuses exposed to tartrazine |
| topic | Tartrazine Teratogenic effect Double skeleton staining Food dye Rat fetuses |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024174877 |
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