Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation
Gas production from thirty samples of feedstuffs (10 samples of corn silage, grass silage, and grass hay, respectively) was assessed in vitro using two methods: the Hohenheim gas test (HGT) and the ANKOM RF Gas Production System (ANKOM). Samples were incubated in buffered rumen fluid. Gas kinetic pa...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2024-03-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/16702 |
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| author | Nejc VALCL Andrej LAVRENČIČ |
| author_facet | Nejc VALCL Andrej LAVRENČIČ |
| author_sort | Nejc VALCL |
| collection | DOAJ |
| description | Gas production from thirty samples of feedstuffs (10 samples of corn silage, grass silage, and grass hay, respectively) was assessed in vitro using two methods: the Hohenheim gas test (HGT) and the ANKOM RF Gas Production System (ANKOM). Samples were incubated in buffered rumen fluid. Gas kinetic parameters were calculated using the Gompertz model. Results revealed significantly lower gas production with the ANKOM compared to the HGT. Significant differences were observed between the HGT and ANKOM in the specific gas production rate (parameter C), maximum fermentation rate (MFR) and gas produced after 24 h of incubation (Gas24) for each feed group. High coefficients of determination (R2) were calculated between the methods for the gas kinetic parameters MFR, Gas24, total potential gas production (parameter B), decrease in the specific gas production rate (parameter A), moderate R2 for C, and low R2 for time of maximum fermentation rate (TMFR). Despite the lower quantities of gas generated with the ANKOM, there are strong correlations in the parameters of gas kinetics that promise the possibility of developing correction models. Future development of such models could position the ANKOM as a viable alternative to HGT, particularly for calculating metabolizable energy and net energy for lactation in feedstuffs. |
| format | Article |
| id | doaj-art-bda83e56e6da434ca9fcdf8d26da74bf |
| institution | OA Journals |
| issn | 1854-1941 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-bda83e56e6da434ca9fcdf8d26da74bf2025-08-20T02:05:13ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412024-03-0112011710.14720/aas.2024.120.1.1670223096Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluationNejc VALCL0Andrej LAVRENČIČ1https://orcid.org/0000-0002-5370-4103University of Ljubljana, Biotechnical Faculty, Department of Animal Science, Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Department of Animal Science, Ljubljana, SloveniaGas production from thirty samples of feedstuffs (10 samples of corn silage, grass silage, and grass hay, respectively) was assessed in vitro using two methods: the Hohenheim gas test (HGT) and the ANKOM RF Gas Production System (ANKOM). Samples were incubated in buffered rumen fluid. Gas kinetic parameters were calculated using the Gompertz model. Results revealed significantly lower gas production with the ANKOM compared to the HGT. Significant differences were observed between the HGT and ANKOM in the specific gas production rate (parameter C), maximum fermentation rate (MFR) and gas produced after 24 h of incubation (Gas24) for each feed group. High coefficients of determination (R2) were calculated between the methods for the gas kinetic parameters MFR, Gas24, total potential gas production (parameter B), decrease in the specific gas production rate (parameter A), moderate R2 for C, and low R2 for time of maximum fermentation rate (TMFR). Despite the lower quantities of gas generated with the ANKOM, there are strong correlations in the parameters of gas kinetics that promise the possibility of developing correction models. Future development of such models could position the ANKOM as a viable alternative to HGT, particularly for calculating metabolizable energy and net energy for lactation in feedstuffs.https://journals.uni-lj.si/aas/article/view/16702animal nutritionrumengas productiongas kinetic parametersankom rf gas production systemhohenheim gas test |
| spellingShingle | Nejc VALCL Andrej LAVRENČIČ Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation Acta Agriculturae Slovenica animal nutrition rumen gas production gas kinetic parameters ankom rf gas production system hohenheim gas test |
| title | Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation |
| title_full | Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation |
| title_fullStr | Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation |
| title_full_unstemmed | Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation |
| title_short | Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation |
| title_sort | rumen gas kinetics a comparative analysis of two in vitro assessment methods for forage evaluation |
| topic | animal nutrition rumen gas production gas kinetic parameters ankom rf gas production system hohenheim gas test |
| url | https://journals.uni-lj.si/aas/article/view/16702 |
| work_keys_str_mv | AT nejcvalcl rumengaskineticsacomparativeanalysisoftwoinvitroassessmentmethodsforforageevaluation AT andrejlavrencic rumengaskineticsacomparativeanalysisoftwoinvitroassessmentmethodsforforageevaluation |