Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding

ABSTRACT Genetic research is unevenly distributed across the globe, with most research done in temperate zones. To better understand the birdlife in an under‐represented, arid subtropical country, Qatar, we blood sampled birds and salvaged tissue from dead birds, then sequenced a mitochondrial marke...

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Main Authors: Emily Rebecca Alison Cramer, Kuei‐Chiu Chen, Arild Johnsen
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Ecology and Evolution
Subjects:
Online Access:https://doi.org/10.1002/ece3.71817
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author Emily Rebecca Alison Cramer
Kuei‐Chiu Chen
Arild Johnsen
author_facet Emily Rebecca Alison Cramer
Kuei‐Chiu Chen
Arild Johnsen
author_sort Emily Rebecca Alison Cramer
collection DOAJ
description ABSTRACT Genetic research is unevenly distributed across the globe, with most research done in temperate zones. To better understand the birdlife in an under‐represented, arid subtropical country, Qatar, we blood sampled birds and salvaged tissue from dead birds, then sequenced a mitochondrial marker (cytochrome c oxidase subunit I, COI, the “DNA barcoding” gene). We chose the DNA barcoding gene because it has previously proven useful for preliminary explorations of evolutionary history. We obtained DNA barcodes for 115 birds (34 species, 12 orders). Our data suggest that the existing DNA barcode reference library, built largely from sampling in the Americas, Europe, and east Asia, is generally sufficient for species identification in Qatar. Based on DNA barcode similarity, Qatar provides overwintering habitat to some species with apparent strong migratory connectivity and others with weaker migratory connectivity. Among locally breeding species, we found no evidence of hybridization between House Sparrows Passer domesticus (n = 16 males) and Spanish Sparrows P. hispaniolensis (n = 14 males), breeding simultaneously and in the same habitats, although in other locations of range overlap, habitat segregation and timing of breeding are hypothesized to be the primary reproductive barrier between them. Our results highlight the benefits of expanding the geographic range of genetic and ecological research.
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spelling doaj-art-bd98a5d9aa794c509309411e07badcf12025-08-20T02:46:14ZengWileyEcology and Evolution2045-77582025-07-01157n/an/a10.1002/ece3.71817Overwintering and Resident Birds in Qatar: Explorations With DNA BarcodingEmily Rebecca Alison Cramer0Kuei‐Chiu Chen1Arild Johnsen2Marine Sciences, Tjärnö Marine Laboratory University of Gothenburg Strömstad SwedenDepartment of Premedical Education Weill Cornell Medicine‐Qatar Education City QatarNatural History Museum University of Oslo Oslo NorwayABSTRACT Genetic research is unevenly distributed across the globe, with most research done in temperate zones. To better understand the birdlife in an under‐represented, arid subtropical country, Qatar, we blood sampled birds and salvaged tissue from dead birds, then sequenced a mitochondrial marker (cytochrome c oxidase subunit I, COI, the “DNA barcoding” gene). We chose the DNA barcoding gene because it has previously proven useful for preliminary explorations of evolutionary history. We obtained DNA barcodes for 115 birds (34 species, 12 orders). Our data suggest that the existing DNA barcode reference library, built largely from sampling in the Americas, Europe, and east Asia, is generally sufficient for species identification in Qatar. Based on DNA barcode similarity, Qatar provides overwintering habitat to some species with apparent strong migratory connectivity and others with weaker migratory connectivity. Among locally breeding species, we found no evidence of hybridization between House Sparrows Passer domesticus (n = 16 males) and Spanish Sparrows P. hispaniolensis (n = 14 males), breeding simultaneously and in the same habitats, although in other locations of range overlap, habitat segregation and timing of breeding are hypothesized to be the primary reproductive barrier between them. Our results highlight the benefits of expanding the geographic range of genetic and ecological research.https://doi.org/10.1002/ece3.71817avifaunacytochrome c oxidase I (COI)DNA barcodingMiddle Eastmigration
spellingShingle Emily Rebecca Alison Cramer
Kuei‐Chiu Chen
Arild Johnsen
Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding
Ecology and Evolution
avifauna
cytochrome c oxidase I (COI)
DNA barcoding
Middle East
migration
title Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding
title_full Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding
title_fullStr Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding
title_full_unstemmed Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding
title_short Overwintering and Resident Birds in Qatar: Explorations With DNA Barcoding
title_sort overwintering and resident birds in qatar explorations with dna barcoding
topic avifauna
cytochrome c oxidase I (COI)
DNA barcoding
Middle East
migration
url https://doi.org/10.1002/ece3.71817
work_keys_str_mv AT emilyrebeccaalisoncramer overwinteringandresidentbirdsinqatarexplorationswithdnabarcoding
AT kueichiuchen overwinteringandresidentbirdsinqatarexplorationswithdnabarcoding
AT arildjohnsen overwinteringandresidentbirdsinqatarexplorationswithdnabarcoding