Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota
Abstract Background Endometriosis, affecting 10% of women in their reproductive years, remains poorly understood. Both individual and environmental unexplained factors are implicated in this heterogenous condition. This study aims to examine the influence of a Western diet on endometriosis lesion de...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s12916-024-03738-9 |
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| author | Guillaume Parpex Benoît Chassaing Mathilde Bourdon Pietro Santulli Ludivine Doridot Marine Thomas Frédéric Batteux Sandrine Chouzenoux Charles Chapron Carole Nicco Louis Marcellin |
| author_facet | Guillaume Parpex Benoît Chassaing Mathilde Bourdon Pietro Santulli Ludivine Doridot Marine Thomas Frédéric Batteux Sandrine Chouzenoux Charles Chapron Carole Nicco Louis Marcellin |
| author_sort | Guillaume Parpex |
| collection | DOAJ |
| description | Abstract Background Endometriosis, affecting 10% of women in their reproductive years, remains poorly understood. Both individual and environmental unexplained factors are implicated in this heterogenous condition. This study aims to examine the influence of a Western diet on endometriosis lesion development in mice and to uncover the mechanisms involved. Methods Mice were fed either a control diet or a Western diet (high in fatty acids and low in fiber) for 4 weeks. Endometriosis was then surgically induced, and lesion development was monitored by ultrasound. After 7 weeks, the mice were sacrificed for analysis of lesion characteristics through RT-qPCR, immunohistochemistry, and flow cytometry. Additionally, the intestinal microbiota was assessed using 16S rRNA gene sequencing. Results Mice on the Western diet developed lesions that were significantly twice as large compared to those on the control diet. These lesions exhibited greater fibrosis and proliferation, alongside enhanced macrophage activity and leptin pathway expression. Changes in the intestinal microbiota were significantly noted after endometriosis induction, regardless of diet. Notably, mice on the Western diet with the most substantial lesions showed a loss of Akkermansia Muciniphila in their intestinal microbiota. Conclusions A Western diet significantly exacerbates lesion size in a mouse model of endometriosis, accompanied by metabolic and immune alterations. The onset of endometriosis also leads to substantial shifts in intestinal microbiota, suggesting a potential link between diet, intestinal health, and endometriosis development. Graphical Abstract |
| format | Article |
| id | doaj-art-bd81e121f7724bde9565f376dc2b8db0 |
| institution | OA Journals |
| issn | 1741-7015 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-bd81e121f7724bde9565f376dc2b8db02025-08-20T02:13:39ZengBMCBMC Medicine1741-70152024-11-0122111510.1186/s12916-024-03738-9Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiotaGuillaume Parpex0Benoît Chassaing1Mathilde Bourdon2Pietro Santulli3Ludivine Doridot4Marine Thomas5Frédéric Batteux6Sandrine Chouzenoux7Charles Chapron8Carole Nicco9Louis Marcellin10Department of Gynecology Obstetrics II and Reproductive Medicine (Professor Chapron), Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Universitaire Paris Centre (HUPC), Centre Hospitalier Universitaire (CHU) CochinInstitut Pasteur, Université Paris Cité, Microbiome-Host Interaction GroupDepartment of Gynecology Obstetrics II and Reproductive Medicine (Professor Chapron), Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Universitaire Paris Centre (HUPC), Centre Hospitalier Universitaire (CHU) CochinDepartment of Gynecology Obstetrics II and Reproductive Medicine (Professor Chapron), Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Universitaire Paris Centre (HUPC), Centre Hospitalier Universitaire (CHU) CochinUniversité Paris Cité, CNRS, Institut CochinUniversité Paris Cité, CNRS, Institut CochinUniversité Paris Cité, CNRS, Institut CochinUniversité Paris Cité, CNRS, Institut CochinDepartment of Gynecology Obstetrics II and Reproductive Medicine (Professor Chapron), Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Universitaire Paris Centre (HUPC), Centre Hospitalier Universitaire (CHU) CochinUniversité Paris Cité, CNRS, Institut CochinDepartment of Gynecology Obstetrics II and Reproductive Medicine (Professor Chapron), Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Universitaire Paris Centre (HUPC), Centre Hospitalier Universitaire (CHU) CochinAbstract Background Endometriosis, affecting 10% of women in their reproductive years, remains poorly understood. Both individual and environmental unexplained factors are implicated in this heterogenous condition. This study aims to examine the influence of a Western diet on endometriosis lesion development in mice and to uncover the mechanisms involved. Methods Mice were fed either a control diet or a Western diet (high in fatty acids and low in fiber) for 4 weeks. Endometriosis was then surgically induced, and lesion development was monitored by ultrasound. After 7 weeks, the mice were sacrificed for analysis of lesion characteristics through RT-qPCR, immunohistochemistry, and flow cytometry. Additionally, the intestinal microbiota was assessed using 16S rRNA gene sequencing. Results Mice on the Western diet developed lesions that were significantly twice as large compared to those on the control diet. These lesions exhibited greater fibrosis and proliferation, alongside enhanced macrophage activity and leptin pathway expression. Changes in the intestinal microbiota were significantly noted after endometriosis induction, regardless of diet. Notably, mice on the Western diet with the most substantial lesions showed a loss of Akkermansia Muciniphila in their intestinal microbiota. Conclusions A Western diet significantly exacerbates lesion size in a mouse model of endometriosis, accompanied by metabolic and immune alterations. The onset of endometriosis also leads to substantial shifts in intestinal microbiota, suggesting a potential link between diet, intestinal health, and endometriosis development. Graphical Abstracthttps://doi.org/10.1186/s12916-024-03738-9EndometriosisDietIntestinal MicrobiotaAkkermansia muciniphila |
| spellingShingle | Guillaume Parpex Benoît Chassaing Mathilde Bourdon Pietro Santulli Ludivine Doridot Marine Thomas Frédéric Batteux Sandrine Chouzenoux Charles Chapron Carole Nicco Louis Marcellin Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota BMC Medicine Endometriosis Diet Intestinal Microbiota Akkermansia muciniphila |
| title | Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota |
| title_full | Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota |
| title_fullStr | Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota |
| title_full_unstemmed | Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota |
| title_short | Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiota |
| title_sort | western diet promotes endometriotic lesion growth in mice and induces depletion of akkermansia muciniphila in intestinal microbiota |
| topic | Endometriosis Diet Intestinal Microbiota Akkermansia muciniphila |
| url | https://doi.org/10.1186/s12916-024-03738-9 |
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