Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease
BackgroundNon-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by the excessive accumulation of lipids as a pathological feature. Previous studies have demonstrated that Rosa roxburghii Tratt. fruit vinegar (RFV) played an important role in intervening in obesity and re...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-08-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1617931/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849235869489168384 |
|---|---|
| author | Yu Wang Yu Wang Rui Lang Lilang Li Lilang Li Yonglan Wen Ming Gao Ming Gao Jie Zhang Juan Yang Juan Yang Qiji Li Qiji Li Li Wang Li Wang Xiaosheng Yang Xiaosheng Yang Xiaolong Wang Chunzhi Xie |
| author_facet | Yu Wang Yu Wang Rui Lang Lilang Li Lilang Li Yonglan Wen Ming Gao Ming Gao Jie Zhang Juan Yang Juan Yang Qiji Li Qiji Li Li Wang Li Wang Xiaosheng Yang Xiaosheng Yang Xiaolong Wang Chunzhi Xie |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | BackgroundNon-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by the excessive accumulation of lipids as a pathological feature. Previous studies have demonstrated that Rosa roxburghii Tratt. fruit vinegar (RFV) played an important role in intervening in obesity and related complications by regulating the intestinal microbiota in high-fat diet mice.MethodsThis study investigated the mechanisms by which RFV improves NAFLD from multiple perspectives. Potential targets were predicted by network pharmacology and molecular docking analyses. Intestinal microbial communities were detected and analyzed using 16S rRNA gene sequencing technology. Liver metabolites were detected and analyzed using ultra high performance liquid chromatography quadrupole-exactive high field-X mass spectrometer (UHPLC-Q-Exactive HF-X) and Progenesis QI software. Hepatic protein expression levels were detected and quantified using Western blotting analysis and gray-value analysis, respectively.ResultsThe results indicated that, RFV could improve the diversity of intestinal microbiota in NAFLD mice, reduce the ratio of Firmicutes to Bacteroidetes (F/B), and reverse the relative abundance of differential bacteria genera related to lipid accumulation and energy metabolism. The intestinal microbiota was correlated with the levels of lipid metabolism and oxidative stress in the serum and liver of mice with NAFLD. The primary bacteria genera involved were Allobaculum, Faecalibaculum, Dubosiella, Blautia, and unclassified_f_Lachnospiraceae. A total of 441 liver metabolites were identified in NAFLD mice and participating in 21 metabolic pathways. Glycerophospholipid metabolism may be an important pathway regulating NAFLD by RFV. Phosphatidylcholines (PC) and lysophosphatidylcholinergic (LPC) metabolites were significantly regulated by RFV and had significant correlation with differential microbiota. RFV may improve NAFLD by regulating lipid synthesis in the adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) pathway. Western blotting analysis showed that, RFV could activate the AMPK phosphorylation, and reduce the expression of fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP-1c), resulting in the inhibition of fatty acids de novo synthesis and lipid accumulation.ConclusionAs a functional food, RFV has been proven to be effective in improving NAFLD. The underlying mechanisms involve the modulation of the intestinal microbiota and metabolites balance, and regulation on lipid disorders through AMPK signaling pathway. |
| format | Article |
| id | doaj-art-bd7da1910758406dbeb94bcd2e0c81b1 |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-bd7da1910758406dbeb94bcd2e0c81b12025-08-20T04:02:32ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-08-011210.3389/fnut.2025.16179311617931Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver diseaseYu Wang0Yu Wang1Rui Lang2Lilang Li3Lilang Li4Yonglan Wen5Ming Gao6Ming Gao7Jie Zhang8Juan Yang9Juan Yang10Qiji Li11Qiji Li12Li Wang13Li Wang14Xiaosheng Yang15Xiaosheng Yang16Xiaolong Wang17Chunzhi Xie18State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaState Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, ChinaNatural Products Research Center of Guizhou Province, Guiyang, ChinaChina National Research Institute of Food & Fermentation Industries Co., Ltd., Beijing, ChinaCollege of Food and Biotechnology Engineering, Xuzhou University of Technology, Xuzhou, ChinaBackgroundNon-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by the excessive accumulation of lipids as a pathological feature. Previous studies have demonstrated that Rosa roxburghii Tratt. fruit vinegar (RFV) played an important role in intervening in obesity and related complications by regulating the intestinal microbiota in high-fat diet mice.MethodsThis study investigated the mechanisms by which RFV improves NAFLD from multiple perspectives. Potential targets were predicted by network pharmacology and molecular docking analyses. Intestinal microbial communities were detected and analyzed using 16S rRNA gene sequencing technology. Liver metabolites were detected and analyzed using ultra high performance liquid chromatography quadrupole-exactive high field-X mass spectrometer (UHPLC-Q-Exactive HF-X) and Progenesis QI software. Hepatic protein expression levels were detected and quantified using Western blotting analysis and gray-value analysis, respectively.ResultsThe results indicated that, RFV could improve the diversity of intestinal microbiota in NAFLD mice, reduce the ratio of Firmicutes to Bacteroidetes (F/B), and reverse the relative abundance of differential bacteria genera related to lipid accumulation and energy metabolism. The intestinal microbiota was correlated with the levels of lipid metabolism and oxidative stress in the serum and liver of mice with NAFLD. The primary bacteria genera involved were Allobaculum, Faecalibaculum, Dubosiella, Blautia, and unclassified_f_Lachnospiraceae. A total of 441 liver metabolites were identified in NAFLD mice and participating in 21 metabolic pathways. Glycerophospholipid metabolism may be an important pathway regulating NAFLD by RFV. Phosphatidylcholines (PC) and lysophosphatidylcholinergic (LPC) metabolites were significantly regulated by RFV and had significant correlation with differential microbiota. RFV may improve NAFLD by regulating lipid synthesis in the adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) pathway. Western blotting analysis showed that, RFV could activate the AMPK phosphorylation, and reduce the expression of fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP-1c), resulting in the inhibition of fatty acids de novo synthesis and lipid accumulation.ConclusionAs a functional food, RFV has been proven to be effective in improving NAFLD. The underlying mechanisms involve the modulation of the intestinal microbiota and metabolites balance, and regulation on lipid disorders through AMPK signaling pathway.https://www.frontiersin.org/articles/10.3389/fnut.2025.1617931/fullRosa roxburghii Tratt.fruit vinegarnon-alcoholic fatty liver diseaseintestinal microbiotametabolomicsAMPK signal pathway |
| spellingShingle | Yu Wang Yu Wang Rui Lang Lilang Li Lilang Li Yonglan Wen Ming Gao Ming Gao Jie Zhang Juan Yang Juan Yang Qiji Li Qiji Li Li Wang Li Wang Xiaosheng Yang Xiaosheng Yang Xiaolong Wang Chunzhi Xie Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease Frontiers in Nutrition Rosa roxburghii Tratt. fruit vinegar non-alcoholic fatty liver disease intestinal microbiota metabolomics AMPK signal pathway |
| title | Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease |
| title_full | Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease |
| title_fullStr | Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease |
| title_full_unstemmed | Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease |
| title_short | Regulation mechanism of Rosa roxburghii Tratt. (Cili) fruit vinegar on non-alcoholic fatty liver disease |
| title_sort | regulation mechanism of rosa roxburghii tratt cili fruit vinegar on non alcoholic fatty liver disease |
| topic | Rosa roxburghii Tratt. fruit vinegar non-alcoholic fatty liver disease intestinal microbiota metabolomics AMPK signal pathway |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1617931/full |
| work_keys_str_mv | AT yuwang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT yuwang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT ruilang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT lilangli regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT lilangli regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT yonglanwen regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT minggao regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT minggao regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT jiezhang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT juanyang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT juanyang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT qijili regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT qijili regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT liwang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT liwang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT xiaoshengyang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT xiaoshengyang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT xiaolongwang regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease AT chunzhixie regulationmechanismofrosaroxburghiitrattcilifruitvinegaronnonalcoholicfattyliverdisease |