STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER
To date, the role of human papillomavirus (HpV) in the development of lung cancer is still under investigation. The available published data do not provide evidence of the involvement of HpV in lung cancer pathogenesis. In Russia, there are no reports regarding the studies of viral contribution to l...
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Russian Academy of Sciences, Tomsk National Research Medical Center
2021-05-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/1755 |
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| author | M. K. Ibragimova M. M. Tsyganov A. M. Pevzner E. O. Rodionov O. V. Cheremisina S. V. Miller S. A. Tuzikov N. V. Litviakov |
| author_facet | M. K. Ibragimova M. M. Tsyganov A. M. Pevzner E. O. Rodionov O. V. Cheremisina S. V. Miller S. A. Tuzikov N. V. Litviakov |
| author_sort | M. K. Ibragimova |
| collection | DOAJ |
| description | To date, the role of human papillomavirus (HpV) in the development of lung cancer is still under investigation. The available published data do not provide evidence of the involvement of HpV in lung cancer pathogenesis. In Russia, there are no reports regarding the studies of viral contribution to lung carcinogenesis.The aim of the present study was to examine the presence of high-risk HpV infection in patients with non-small cell lung cancer (NSCLC).Material and Methods. The study included 30 patients with histologically verified stage IIBIIIA NSCLC. Normal and tumor lung tissue samples as well as whole blood samples from each patient were used. dNA was isolated from tissue samples using the QIAamp dNA mini Kit (Qiagen, Germany). Amplisens® reagent kits (Moscow, Russia) were used for dNA isolation and genotyping.Results. The presence of HpV dNA was detected in 3 (10.0 %) patients. HpV dNA was not detected in 27 (90.0 %) patients. HpV-16 genotype was observed in 2 HpV-positive patients, and HpV-33 was observed in 1 patient. A clinically insignificant viral load was found in almost all HpV-positive samples. The assessment of the virus integration in HpV16-positive lung cancer patients revealed mixed and integrated forms of the virus in the tumor tissue of these patients. The 5-year metastasis-free survival rate in all HpV-positive patients was 100 %.Conclusion. Our data showed HpV dNA prevalence rate of 10 % among NSCLC patients in the Russian population. The presence/absence of a viral component for lung cancer patients is a clinically significant parameter. |
| format | Article |
| id | doaj-art-bd7d6198040e4188a5efcd7ef05f4cfe |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2021-05-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-bd7d6198040e4188a5efcd7ef05f4cfe2025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682021-05-01202536010.21294/1814-4861-2021-20-2-53-60844STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCERM. K. Ibragimova0M. M. Tsyganov1A. M. Pevzner2E. O. Rodionov3O. V. Cheremisina4S. V. Miller5S. A. Tuzikov6N. V. Litviakov7Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; Tomsk State UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; Tomsk State UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; Siberian State University, Ministry of Healthcare of the Russian FederationCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesTo date, the role of human papillomavirus (HpV) in the development of lung cancer is still under investigation. The available published data do not provide evidence of the involvement of HpV in lung cancer pathogenesis. In Russia, there are no reports regarding the studies of viral contribution to lung carcinogenesis.The aim of the present study was to examine the presence of high-risk HpV infection in patients with non-small cell lung cancer (NSCLC).Material and Methods. The study included 30 patients with histologically verified stage IIBIIIA NSCLC. Normal and tumor lung tissue samples as well as whole blood samples from each patient were used. dNA was isolated from tissue samples using the QIAamp dNA mini Kit (Qiagen, Germany). Amplisens® reagent kits (Moscow, Russia) were used for dNA isolation and genotyping.Results. The presence of HpV dNA was detected in 3 (10.0 %) patients. HpV dNA was not detected in 27 (90.0 %) patients. HpV-16 genotype was observed in 2 HpV-positive patients, and HpV-33 was observed in 1 patient. A clinically insignificant viral load was found in almost all HpV-positive samples. The assessment of the virus integration in HpV16-positive lung cancer patients revealed mixed and integrated forms of the virus in the tumor tissue of these patients. The 5-year metastasis-free survival rate in all HpV-positive patients was 100 %.Conclusion. Our data showed HpV dNA prevalence rate of 10 % among NSCLC patients in the Russian population. The presence/absence of a viral component for lung cancer patients is a clinically significant parameter.https://www.siboncoj.ru/jour/article/view/1755hpvlung cancerprognosisphysical status of the viruslung cancer pathogenesis |
| spellingShingle | M. K. Ibragimova M. M. Tsyganov A. M. Pevzner E. O. Rodionov O. V. Cheremisina S. V. Miller S. A. Tuzikov N. V. Litviakov STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER Сибирский онкологический журнал hpv lung cancer prognosis physical status of the virus lung cancer pathogenesis |
| title | STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER |
| title_full | STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER |
| title_fullStr | STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER |
| title_full_unstemmed | STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER |
| title_short | STUDY OF THE HUMAN PAPILLOMA VIRUS PREVALENCE AND ITS PHYSICAL STATUS IN PATIENS WITH NON-SMALL CELL LUNG CANCER |
| title_sort | study of the human papilloma virus prevalence and its physical status in patiens with non small cell lung cancer |
| topic | hpv lung cancer prognosis physical status of the virus lung cancer pathogenesis |
| url | https://www.siboncoj.ru/jour/article/view/1755 |
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