Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds
To study the factors affecting <i>Penaeus vannamei</i> production in small-scale greenhouse ponds, four ponds in Jiangmen, Guangdong Province, China were selected. This study investigated the variation in the characteristics of bacterial communities and pathogens in pond water and shrimp...
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2025-02-01
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| author | Siyu Wu Haochang Su Lei Su Yucheng Cao Guoliang Wen Yu Xu Bin Shen Shanshan Wu Yuting Su Xiaojuan Hu |
| author_facet | Siyu Wu Haochang Su Lei Su Yucheng Cao Guoliang Wen Yu Xu Bin Shen Shanshan Wu Yuting Su Xiaojuan Hu |
| author_sort | Siyu Wu |
| collection | DOAJ |
| description | To study the factors affecting <i>Penaeus vannamei</i> production in small-scale greenhouse ponds, four ponds in Jiangmen, Guangdong Province, China were selected. This study investigated the variation in the characteristics of bacterial communities and pathogens in pond water and shrimp intestines, as well as water quality factors during the culture stage. Multivariate linear regression equations were used to analyse the potential factors affecting production. The nitrite concentration reached its peak in the mid-culture stage, with a maximum of 16.3 mg·L<sup>−1</sup>, whereas total nitrogen and salinity were highest in the late culture stage, reaching 48.4 mg·L<sup>−1</sup> and 26, respectively. The dominant bacteria in the pond water were <i>Marivita</i> and Rhodobacteraceae, whereas in the shrimp intestines, they were <i>Bacillus</i> and <i>Candidatus Bacilloplasma.</i> The nitrifying bacteria in the pond water were dominated by <i>Nitrosomonas</i> and <i>Nitrobacter</i>. Pathogens detected in the pond water included acute hepatopancreatic necrosis disease (AHPND), <i>Enterocytozoon hepatopenaei</i> (EHP), and white spot syndrome virus (WSSV). The counts of EHP and the relative abundance of <i>Ardenticatenales_norank</i> and <i>Marivita</i> in the pond were the main factors affecting the shrimp production (<i>p</i> < 0.01). This study indicates that establishing optimal bacterial communities, such as <i>Marivita</i>, <i>Nitrobacter</i>, and Rhodobacteraceae, and controlling the counts of EHP and AHPND pathogens is crucial for regulating the pond environment and enhancing production. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-bd7a64e5d73042adbf2dce02866a71682025-08-20T01:48:53ZengMDPI AGMicroorganisms2076-26072025-02-0113354610.3390/microorganisms13030546Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse PondsSiyu Wu0Haochang Su1Lei Su2Yucheng Cao3Guoliang Wen4Yu Xu5Bin Shen6Shanshan Wu7Yuting Su8Xiaojuan Hu9South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510300, ChinaGuangzhou Resource Environmental Protection Technology Co., Ltd., Guangzhou 510699, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510300, ChinaNational Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaGuangzhou Resource Environmental Protection Technology Co., Ltd., Guangzhou 510699, ChinaGuangzhou Resource Environmental Protection Technology Co., Ltd., Guangzhou 510699, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510300, ChinaTo study the factors affecting <i>Penaeus vannamei</i> production in small-scale greenhouse ponds, four ponds in Jiangmen, Guangdong Province, China were selected. This study investigated the variation in the characteristics of bacterial communities and pathogens in pond water and shrimp intestines, as well as water quality factors during the culture stage. Multivariate linear regression equations were used to analyse the potential factors affecting production. The nitrite concentration reached its peak in the mid-culture stage, with a maximum of 16.3 mg·L<sup>−1</sup>, whereas total nitrogen and salinity were highest in the late culture stage, reaching 48.4 mg·L<sup>−1</sup> and 26, respectively. The dominant bacteria in the pond water were <i>Marivita</i> and Rhodobacteraceae, whereas in the shrimp intestines, they were <i>Bacillus</i> and <i>Candidatus Bacilloplasma.</i> The nitrifying bacteria in the pond water were dominated by <i>Nitrosomonas</i> and <i>Nitrobacter</i>. Pathogens detected in the pond water included acute hepatopancreatic necrosis disease (AHPND), <i>Enterocytozoon hepatopenaei</i> (EHP), and white spot syndrome virus (WSSV). The counts of EHP and the relative abundance of <i>Ardenticatenales_norank</i> and <i>Marivita</i> in the pond were the main factors affecting the shrimp production (<i>p</i> < 0.01). This study indicates that establishing optimal bacterial communities, such as <i>Marivita</i>, <i>Nitrobacter</i>, and Rhodobacteraceae, and controlling the counts of EHP and AHPND pathogens is crucial for regulating the pond environment and enhancing production.https://www.mdpi.com/2076-2607/13/3/546small-scale greenhouse pondmicrobial communitywater qualityshrimp production<i>Penaeus vannamei</i> |
| spellingShingle | Siyu Wu Haochang Su Lei Su Yucheng Cao Guoliang Wen Yu Xu Bin Shen Shanshan Wu Yuting Su Xiaojuan Hu Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds Microorganisms small-scale greenhouse pond microbial community water quality shrimp production <i>Penaeus vannamei</i> |
| title | Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds |
| title_full | Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds |
| title_fullStr | Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds |
| title_full_unstemmed | Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds |
| title_short | Variation of Microorganisms and Water Quality, and Their Impacts on the Production of <i>Penaeus vannamei</i> in Small-Scale Greenhouse Ponds |
| title_sort | variation of microorganisms and water quality and their impacts on the production of i penaeus vannamei i in small scale greenhouse ponds |
| topic | small-scale greenhouse pond microbial community water quality shrimp production <i>Penaeus vannamei</i> |
| url | https://www.mdpi.com/2076-2607/13/3/546 |
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