Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria
Abstract Study was designed to determine frequency and distribution of unsuccessful test results and factors associated with high Error rates (ER) in Truenat sites in Nigeria. A retrospective cross-sectional study was done involving secondary data analysis of 38 Truenat facilities between January an...
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Nature Portfolio
2025-06-01
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| Online Access: | https://doi.org/10.1038/s41598-025-04199-7 |
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| author | Jamiu Olabamiji Austin Ihesie Kingsley Ochei Nkiru Nwokoye Rupert Eneogu Omotayo Salau Peter Nwadike Ikechukwu Anaedobe Jelilat Salau Bethrand Odume Aderonke Agbaje Debby Nongo Omosalewa Oyelaran Wayne van Gemert Lucy Mupfumi Elom Emeka Chukwuma Anyaike Temitayo Lagundoye Edmund Ndudi Ossai |
| author_facet | Jamiu Olabamiji Austin Ihesie Kingsley Ochei Nkiru Nwokoye Rupert Eneogu Omotayo Salau Peter Nwadike Ikechukwu Anaedobe Jelilat Salau Bethrand Odume Aderonke Agbaje Debby Nongo Omosalewa Oyelaran Wayne van Gemert Lucy Mupfumi Elom Emeka Chukwuma Anyaike Temitayo Lagundoye Edmund Ndudi Ossai |
| author_sort | Jamiu Olabamiji |
| collection | DOAJ |
| description | Abstract Study was designed to determine frequency and distribution of unsuccessful test results and factors associated with high Error rates (ER) in Truenat sites in Nigeria. A retrospective cross-sectional study was done involving secondary data analysis of 38 Truenat facilities between January and December 2022. Socio-demographic information of key Truenat lab staff were collected. High ER was determined by proportion of health facilities with > 3% unsuccessful tests. A total of 56,799 Trueprep DNA extractions were conducted, unsuccessful tests were 1043. (ER = 1.8%). For Truenat MTB, 56,749 tests were conducted and 1350 were unsuccessful. (ER = 2.4%). High Trueprep DNA extraction test errors was recorded in 23.7% of facilities and 31.6% for Truenat MTB tests. No factor significantly influenced any of the test errors. Unsuccessful Trueprep test error codes were 1021 while unsuccessful Truelab test error codes were 1198. Highest Trueprep error recorded was cartridge error (44.1%), while highest Truelab error was internal control not amplifying in PCR (78%). Implementation of Truenat as a new tool for TB diagnosis in Nigeria is associated with low ERs. It is recommended that Truenat training curriculum in-country be updated to focus on and address the possible underlying causes of these errors as the country plans for scale up. |
| format | Article |
| id | doaj-art-bd68cd5202a640eaa5a535310042be00 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-bd68cd5202a640eaa5a535310042be002025-08-20T03:10:28ZengNature PortfolioScientific Reports2045-23222025-06-011511910.1038/s41598-025-04199-7Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in NigeriaJamiu Olabamiji0Austin Ihesie1Kingsley Ochei2Nkiru Nwokoye3Rupert Eneogu4Omotayo Salau5Peter Nwadike6Ikechukwu Anaedobe7Jelilat Salau8Bethrand Odume9Aderonke Agbaje10Debby Nongo11Omosalewa Oyelaran12Wayne van Gemert13Lucy Mupfumi14Elom Emeka15Chukwuma Anyaike16Temitayo Lagundoye17Edmund Ndudi Ossai18IHV-NigeriaHIV AIDS & TB Office, USAID NigeriaUSAID Leap ProjectKNCV NigeriaHIV AIDS & TB Office, USAID NigeriaKNCV NigeriaKNCV NigeriaKNCV NigeriaIHV-NigeriaKNCV NigeriaIHV-NigeriaHIV AIDS & TB Office, USAID NigeriaHIV AIDS & TB Office, USAID NigeriaSTOP TB PartnershipSTOP TB PartnershipFederal Ministry of HealthFederal Ministry of HealthIndependent ConsultantDepartment of Community Medicine, College of Health Sciences, Ebonyi State UniversityAbstract Study was designed to determine frequency and distribution of unsuccessful test results and factors associated with high Error rates (ER) in Truenat sites in Nigeria. A retrospective cross-sectional study was done involving secondary data analysis of 38 Truenat facilities between January and December 2022. Socio-demographic information of key Truenat lab staff were collected. High ER was determined by proportion of health facilities with > 3% unsuccessful tests. A total of 56,799 Trueprep DNA extractions were conducted, unsuccessful tests were 1043. (ER = 1.8%). For Truenat MTB, 56,749 tests were conducted and 1350 were unsuccessful. (ER = 2.4%). High Trueprep DNA extraction test errors was recorded in 23.7% of facilities and 31.6% for Truenat MTB tests. No factor significantly influenced any of the test errors. Unsuccessful Trueprep test error codes were 1021 while unsuccessful Truelab test error codes were 1198. Highest Trueprep error recorded was cartridge error (44.1%), while highest Truelab error was internal control not amplifying in PCR (78%). Implementation of Truenat as a new tool for TB diagnosis in Nigeria is associated with low ERs. It is recommended that Truenat training curriculum in-country be updated to focus on and address the possible underlying causes of these errors as the country plans for scale up.https://doi.org/10.1038/s41598-025-04199-7TruenatTrueprepTruelabUnsuccessful testsHigh errorNigeria |
| spellingShingle | Jamiu Olabamiji Austin Ihesie Kingsley Ochei Nkiru Nwokoye Rupert Eneogu Omotayo Salau Peter Nwadike Ikechukwu Anaedobe Jelilat Salau Bethrand Odume Aderonke Agbaje Debby Nongo Omosalewa Oyelaran Wayne van Gemert Lucy Mupfumi Elom Emeka Chukwuma Anyaike Temitayo Lagundoye Edmund Ndudi Ossai Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria Scientific Reports Truenat Trueprep Truelab Unsuccessful tests High error Nigeria |
| title | Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria |
| title_full | Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria |
| title_fullStr | Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria |
| title_full_unstemmed | Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria |
| title_short | Pilot implementation result of frequency, distribution, and factors associated with unsuccessful tests and error rates at Truenat sites in Nigeria |
| title_sort | pilot implementation result of frequency distribution and factors associated with unsuccessful tests and error rates at truenat sites in nigeria |
| topic | Truenat Trueprep Truelab Unsuccessful tests High error Nigeria |
| url | https://doi.org/10.1038/s41598-025-04199-7 |
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