Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp)
Objectives: Polymethylmethacrylate (PMMA) has been widely used, but it has several fallback properties in its interaction with bone tissue, so the addition of hydroxyapatite (HAp) material aims to improve the biocompatibility, regeneration process, and osteointegration of bone implants. The HAp mate...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426823000180 |
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| author | Claudia Michelle Darjanki Jonathan Steven Hananta Chiquita Prahasanti Noer Ulfah Banun Kusumawardani I Komang Evan Wijaksana Mohammed Aljunaid Anord Nkuba |
| author_facet | Claudia Michelle Darjanki Jonathan Steven Hananta Chiquita Prahasanti Noer Ulfah Banun Kusumawardani I Komang Evan Wijaksana Mohammed Aljunaid Anord Nkuba |
| author_sort | Claudia Michelle Darjanki |
| collection | DOAJ |
| description | Objectives: Polymethylmethacrylate (PMMA) has been widely used, but it has several fallback properties in its interaction with bone tissue, so the addition of hydroxyapatite (HAp) material aims to improve the biocompatibility, regeneration process, and osteointegration of bone implants. The HAp material can be sourced from bovine bone and processed through Good Manufacturing Practice from Tissue Bank (HApGMP), and from limestone (CaCO3) processed by Balai Besar Keramik (HApBBK).This study was to observe the expression of vascular endothelial growth factor (VEGF) and Bone morphogenetic protein-2 (BMP2) in cultured osteoblasts exposed to PMMA-HApGMP and PMMA-HApBBK as implant candidate materials. Methods: Sample of PMMA and HAp materials with a mixture of PMMA and HApBBK in the first group and a mixture of PMMA and HApGMP in the second group. Twenty-four fetal rat calvarie osteoblast cell cultures were randomly divided into 6 groups: 7- and 14-day control group, 7 and 14 days PMMA-HApGMP group, 7 and 14 days PMMA-HApBBK group. The expression of VEGF and BMP-2 was seen by immunocytochemical examination. Results: The one-way ANOVA with a significance value of 0.000 (p < 0.05). BMP-2 and VEGF expression was increased in the 7- and 14-days groups after exposure to PMMA-HApGMP and PMMA-HApBBK. Conclusion: The application of PMMA-HApGMP and PMMA-HApBBK showed an increase in the expression of VEGF and BMP-2 in osteoblast cell cultures which indicates a potential increase in the accelerated angiogenesis and osteogenesis in the bone regeneration process of bone implants. |
| format | Article |
| id | doaj-art-bd5daf7107634c79bf0d4a9aefaad291 |
| institution | DOAJ |
| issn | 2212-4268 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-bd5daf7107634c79bf0d4a9aefaad2912025-08-20T02:50:26ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682023-03-0113224324810.1016/j.jobcr.2023.02.006Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp)Claudia Michelle Darjanki0Jonathan Steven Hananta1Chiquita Prahasanti2Noer Ulfah3Banun Kusumawardani4I Komang Evan Wijaksana5Mohammed Aljunaid6Anord Nkuba7Periodontology Department, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaPeriodontology Department, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaDepartment of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; Corresponding author. Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Moestopo No 47, Surabaya, 60132, Indonesia.Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Biomedical Sciences, Faculty of Dentistry, University of Jember, Surabaya, IndonesiaDepartment of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDoctoral Program of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Dental and Oral Medicine, Faculty of Medicine, Taiz University, Taiz, YemenAqua Farms Organization. Sinza, Dar Es Salaam, Tanzania; European Marine Biological Resource, Ghent University, BelgiumObjectives: Polymethylmethacrylate (PMMA) has been widely used, but it has several fallback properties in its interaction with bone tissue, so the addition of hydroxyapatite (HAp) material aims to improve the biocompatibility, regeneration process, and osteointegration of bone implants. The HAp material can be sourced from bovine bone and processed through Good Manufacturing Practice from Tissue Bank (HApGMP), and from limestone (CaCO3) processed by Balai Besar Keramik (HApBBK).This study was to observe the expression of vascular endothelial growth factor (VEGF) and Bone morphogenetic protein-2 (BMP2) in cultured osteoblasts exposed to PMMA-HApGMP and PMMA-HApBBK as implant candidate materials. Methods: Sample of PMMA and HAp materials with a mixture of PMMA and HApBBK in the first group and a mixture of PMMA and HApGMP in the second group. Twenty-four fetal rat calvarie osteoblast cell cultures were randomly divided into 6 groups: 7- and 14-day control group, 7 and 14 days PMMA-HApGMP group, 7 and 14 days PMMA-HApBBK group. The expression of VEGF and BMP-2 was seen by immunocytochemical examination. Results: The one-way ANOVA with a significance value of 0.000 (p < 0.05). BMP-2 and VEGF expression was increased in the 7- and 14-days groups after exposure to PMMA-HApGMP and PMMA-HApBBK. Conclusion: The application of PMMA-HApGMP and PMMA-HApBBK showed an increase in the expression of VEGF and BMP-2 in osteoblast cell cultures which indicates a potential increase in the accelerated angiogenesis and osteogenesis in the bone regeneration process of bone implants.http://www.sciencedirect.com/science/article/pii/S2212426823000180BMP-2Dental implantHydroxyapatitePolymethylmethacrylateVEGFPMMA-HAp |
| spellingShingle | Claudia Michelle Darjanki Jonathan Steven Hananta Chiquita Prahasanti Noer Ulfah Banun Kusumawardani I Komang Evan Wijaksana Mohammed Aljunaid Anord Nkuba Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp) Journal of Oral Biology and Craniofacial Research BMP-2 Dental implant Hydroxyapatite Polymethylmethacrylate VEGF PMMA-HAp |
| title | Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp) |
| title_full | Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp) |
| title_fullStr | Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp) |
| title_full_unstemmed | Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp) |
| title_short | Expression of VEGF and BMP-2 in Osteoblast cells exposed to a combination of polymethylmethacrylate (PMMA) and hydroxyapatite (HAp) |
| title_sort | expression of vegf and bmp 2 in osteoblast cells exposed to a combination of polymethylmethacrylate pmma and hydroxyapatite hap |
| topic | BMP-2 Dental implant Hydroxyapatite Polymethylmethacrylate VEGF PMMA-HAp |
| url | http://www.sciencedirect.com/science/article/pii/S2212426823000180 |
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