Determination of S-genotypes of 13 Chinese pear cultivars based on sequence analysis
To identify S-RNase genes and establish the correlation between the S-RNase genes and S-genotypes of Chinese pears, 13 cultivars were investigated. Genomic PCR with primers derived from the conserved nucleotide sequence of Japanese S-RNases, was carried out. As a result, 13 S-alleles were amplified....
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.004 |
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| Summary: | To identify S-RNase genes and establish the correlation between the S-RNase genes and S-genotypes of Chinese pears, 13 cultivars were investigated. Genomic PCR with primers derived from the conserved nucleotide sequence of Japanese S-RNases, was carried out. As a result, 13 S-alleles were amplified. Comparison of the nucleotide and amino acid sequences between the 13 S-alleles and S-alleles deposited under Genbank databases revealed that 11 S-alleles corresponded to S<sub>1</sub>, S<sub>7</sub>, S<sub>12</sub>, S<sub>15</sub>, S<sub>16</sub>, S<sub>18</sub>, S<sub>19</sub>, S<sub>22</sub>, S<sub>27</sub>, S<sub>29</sub> and S<sub>34</sub>-RNases, and the other two were new S-alleles (designated as S<sub>37</sub> and S<sub>38</sub>). A pairwise comparison among the 13 S-alleles revealed an identity of 58% (between S<sub>18</sub> and S<sub>27</sub>) to 94% (between S<sub>7</sub> and S<sub>27</sub>, S<sub>12</sub> and S<sub>19</sub>, S<sub>15</sub> and S<sub>37</sub>, S<sub>15</sub> and S<sub>38</sub>) at the deduced amino acid level. In addition. S<sub>19</sub> was found to be the most frequent S-allele in Chinese pears. Based on restriction sites analysis, a rapid and economical method was developed for the identification of S<sub>19</sub> without going to the effort of sequencing but using the digestion with S<sub>19</sub>-specific restriction endonuclease Afl Ⅱ. The 13 cultivars were genotyped as follows: 'Bingtang' (S<sub>16</sub>S<sub>19</sub>), ‘Liuleng' (S<sub>16</sub>S<sub>19</sub>), ‘Jinxiang' (S<sub>34</sub>S<sub>37</sub>), 'Esu' (S<sub>15</sub>S<sub>38</sub>), ‘Mili' (S<sub>19</sub>S<sub>29</sub>), 'Tianchengzi' (S<sub>7</sub>S<sub>12</sub>), ‘Daqingpi' (S<sub>19</sub> S<sub>34</sub>), ‘Qiubai' (S<sub>19</sub>S<sub>34</sub>), ‘Zisu' (S<sub>19</sub>S<sub>34</sub>), ‘Huachangba' (S<sub>19</sub>S<sub>22</sub>), 'Guanyangxueli' (S<sub>18</sub>S<sub>27</sub>), ‘Zaomi' (S<sub>19</sub>S<sub>29</sub>) and ‘Qingmian' (S<sub>1</sub>S<sub>18</sub>). Elucidation of S-genotypes of Chinese pears would contribute to higher and stable yields in the orchards and a more efficient breeding program. |
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| ISSN: | 1008-9209 2097-5155 |