Intraguild predation between Pardosa pseudoannulata and Paederus fuscipes in ratoon rice fields and its influencing factors
The intraguild predation (IGP) level among the main generalist natural enemies is related to their population occurrence and the effectiveness of “green control” strategies for insect pests in agroecosystem. In this study, the detection systems of quantitative real-time polymerase chain reaction (qP...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2022-12-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.07.041 |
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| Summary: | The intraguild predation (IGP) level among the main generalist natural enemies is related to their population occurrence and the effectiveness of “green control” strategies for insect pests in agroecosystem. In this study, the detection systems of quantitative real-time polymerase chain reaction (qPCR) probe were first established and then optimized for analyzing the DNA of Pardosa pseudoannulata and Paederus fuscipes in ratoon rice fields. Then the detection systems were used to analyze the IGP levels between the two predators in ratoon rice fields. On this basis, the IGP and their biological and abiotic factors affecting the IGP level were studied under indoor conditions. The results showed that the primers and probes designed for the two predators had strong specificity. The established and optimized qPCR detection systems had good amplification repeatability and high sensitivity for the target genes of the two predators. After the system was used to detect 1 527 individuals of the two predators from ratoon rice fields and the indoor IGP tests, it was found that there was a ubiquitous and strong bidirectional IGP between the two predators in the rice field. The role transformation of intraguild predator or prey in the IGP and the influence of ambient temperature on the IGP level were closely related to their relative body sizes (developmental stages). The IGP level can be affected through extraguild preys and abiotic factors and their combination to various extents. These results enrich the IGP theory among arthropod predators in the rice ecosystem, and lay good foundations for further IGP study of natural enemies. |
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| ISSN: | 1008-9209 2097-5155 |