Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota
Scope: High-fat diet (HFD) induces imbalance in the small intestine environment, where fat digestion and absorption mainly take place. This study aimed to elucidate the mechanisms by which Lonicera caerulea polyphenols (LCP) might inhibit fat absorption, from the perspective of small intestine micro...
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| Format: | Article |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002324 |
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| author | Yuehua Wang Ningxuan Gao Andrea Nieto-Veloza Lingxi Zhou Xiyun Sun Xu Si Jinlong Tian Yang Lin Xinyao Jiao Bin Li |
| author_facet | Yuehua Wang Ningxuan Gao Andrea Nieto-Veloza Lingxi Zhou Xiyun Sun Xu Si Jinlong Tian Yang Lin Xinyao Jiao Bin Li |
| author_sort | Yuehua Wang |
| collection | DOAJ |
| description | Scope: High-fat diet (HFD) induces imbalance in the small intestine environment, where fat digestion and absorption mainly take place. This study aimed to elucidate the mechanisms by which Lonicera caerulea polyphenols (LCP) might inhibit fat absorption, from the perspective of small intestine microbiota and epithelial barrier integrity. Methods and results: Male Sprague-Dawley rats were given HFD with or without co-administration of LCP for 8 weeks. The results showed that LCP supplementation significantly decreased the levels of serum triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C), and increased the contents of fecal sterols, in HFD rats. LCP also inhibited the dysfunction of the small intestine epithelial barrier, via alleviating the oxidative stress activated by Nrf2-ARE pathway, and by modulating the expressions of pro-inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), nuclear factor kappa-B p65 (NF-κB p65) and inducible nitric oxide synthase (iNOS) in the small intestine. Additionally, LCP administration restored the balance in small intestine microbiota and increased the abundance of the specific bacteria, such as Lactobacillus, involved in fat absorption. Conclusion: Our results demonstrated that LCP may be beneficial to inhibit fat absorption. The mechanism seems to be associated with the protection of the epithelial barrier integrity and the modulation of specific bacteria in the small intestine. |
| format | Article |
| id | doaj-art-bd4e5e4831944a2ea0f150dc12421240 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-bd4e5e4831944a2ea0f150dc124212402025-02-03T07:06:24ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112413091322Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiotaYuehua Wang0Ningxuan Gao1Andrea Nieto-Veloza2Lingxi Zhou3Xiyun Sun4Xu Si5Jinlong Tian6Yang Lin7Xinyao Jiao8Bin Li9College of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, Shenyang 110161, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, Shenyang 110161, ChinaInstituto de Ciencia y Tecnología de Alimentos, Universidad Nacional de Colombia, Bogotá 111311, ColombiaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, Shenyang 110161, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, Shenyang 110161, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, Shenyang 110161, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Corresponding authors at: College of Food Science, Shenyang Agricultural University, Shenyang 110161, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, Shenyang 110161, China; Corresponding authors at: College of Food Science, Shenyang Agricultural University, Shenyang 110161, ChinaScope: High-fat diet (HFD) induces imbalance in the small intestine environment, where fat digestion and absorption mainly take place. This study aimed to elucidate the mechanisms by which Lonicera caerulea polyphenols (LCP) might inhibit fat absorption, from the perspective of small intestine microbiota and epithelial barrier integrity. Methods and results: Male Sprague-Dawley rats were given HFD with or without co-administration of LCP for 8 weeks. The results showed that LCP supplementation significantly decreased the levels of serum triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C), and increased the contents of fecal sterols, in HFD rats. LCP also inhibited the dysfunction of the small intestine epithelial barrier, via alleviating the oxidative stress activated by Nrf2-ARE pathway, and by modulating the expressions of pro-inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), nuclear factor kappa-B p65 (NF-κB p65) and inducible nitric oxide synthase (iNOS) in the small intestine. Additionally, LCP administration restored the balance in small intestine microbiota and increased the abundance of the specific bacteria, such as Lactobacillus, involved in fat absorption. Conclusion: Our results demonstrated that LCP may be beneficial to inhibit fat absorption. The mechanism seems to be associated with the protection of the epithelial barrier integrity and the modulation of specific bacteria in the small intestine.http://www.sciencedirect.com/science/article/pii/S2213453022002324Lonicera caerulea berriesPolyphenolsGut microbiotaOxidative stressIntestinal epithelial barrier |
| spellingShingle | Yuehua Wang Ningxuan Gao Andrea Nieto-Veloza Lingxi Zhou Xiyun Sun Xu Si Jinlong Tian Yang Lin Xinyao Jiao Bin Li Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota Food Science and Human Wellness Lonicera caerulea berries Polyphenols Gut microbiota Oxidative stress Intestinal epithelial barrier |
| title | Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota |
| title_full | Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota |
| title_fullStr | Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota |
| title_full_unstemmed | Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota |
| title_short | Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota |
| title_sort | lonicera caerulea polyphenols inhibit fat absorption by regulating nrf2 are pathway mediated epithelial barrier dysfunction and special microbiota |
| topic | Lonicera caerulea berries Polyphenols Gut microbiota Oxidative stress Intestinal epithelial barrier |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002324 |
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