Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes

Lijing Huo,1,2 Xuexin Liu,2 Nan Ding,2 Hairui Zhang,1 Shiyu Hou,1 Jintian He,1 Baohua Zhao1 1College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, People’s Republic of China; 2Department of Medical Laboratory, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic of ChinaCo...

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Main Authors: Huo L, Liu X, Ding N, Zhang H, Hou S, He J, Zhao B
Format: Article
Language:English
Published: Dove Medical Press 2025-02-01
Series:Diabetes, Metabolic Syndrome and Obesity
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Online Access:https://www.dovepress.com/pyroptosis-mediator-gsdmd-in-plasma-a-promising-biomarker-for-early-di-peer-reviewed-fulltext-article-DMSO
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author Huo L
Liu X
Ding N
Zhang H
Hou S
He J
Zhao B
author_facet Huo L
Liu X
Ding N
Zhang H
Hou S
He J
Zhao B
author_sort Huo L
collection DOAJ
description Lijing Huo,1,2 Xuexin Liu,2 Nan Ding,2 Hairui Zhang,1 Shiyu Hou,1 Jintian He,1 Baohua Zhao1 1College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, People’s Republic of China; 2Department of Medical Laboratory, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic of ChinaCorrespondence: Jintian He; Baohua Zhao, College of Life Science, Hebei Normal University, No. 20 Road East of 2nd Ring South, Shijiazhuang City, Hebei Province, 050024, People’s Republic of China, Tel +86 311 80786666 ; +86 311 80787777, Email 576477418@qq.com; zhaobaohua@mail.hebtu.edu.cnObjective: This study investigates the potential of plasma Gasdermin D (GSDMD) as a novel biomarker for early diagnosis and monitoring of type 2 diabetes mellitus.Methods: We conducted a comparative analysis of clinical indicators among newly diagnosed diabetes patients, those with prediabetes, and healthy controls, finding significantly elevated plasma GSDMD levels in the diabetes group (P < 0.05).Results: Correlation analyses revealed that GSDMD levels were positively associated with inflammatory markers and indicators of insulin resistance, while negatively correlating with HOMA-β (P < 0.001). Logistic regression analysis identified GSDMD, IL-6, and CRP as independent risk factors for type 2 diabetes (P < 0.001). The area under the ROC curve for plasma GSDMD was 0.988, indicating superior diagnostic capability compared to traditional markers like CRP (0.902) and IL-6 (0.857). With a threshold of 17.67 pg/mL, plasma GSDMD exhibited a sensitivity of 93.9% and specificity of 98.0%.Conclusion: These findings suggest that plasma GSDMD not only reflects early metabolic abnormalities associated with type 2 diabetes but also holds promise as a therapeutic target to mitigate inflammatory responses and improve insulin resistance. Further large-scale clinical studies are warranted to validate its diagnostic utility and enhance clinical applications.Keywords: biomarker discovery, gasdermin D, early diagnosis, type 2 diabetes mellitus, plasma biomarker, prediabetes
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issn 1178-7007
language English
publishDate 2025-02-01
publisher Dove Medical Press
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series Diabetes, Metabolic Syndrome and Obesity
spelling doaj-art-bd400b2ed34b4e8c9fa3eb30ada14ffb2025-08-20T03:11:37ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072025-02-01Volume 18453464100176Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 DiabetesHuo LLiu XDing NZhang HHou SHe JZhao BLijing Huo,1,2 Xuexin Liu,2 Nan Ding,2 Hairui Zhang,1 Shiyu Hou,1 Jintian He,1 Baohua Zhao1 1College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, People’s Republic of China; 2Department of Medical Laboratory, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic of ChinaCorrespondence: Jintian He; Baohua Zhao, College of Life Science, Hebei Normal University, No. 20 Road East of 2nd Ring South, Shijiazhuang City, Hebei Province, 050024, People’s Republic of China, Tel +86 311 80786666 ; +86 311 80787777, Email 576477418@qq.com; zhaobaohua@mail.hebtu.edu.cnObjective: This study investigates the potential of plasma Gasdermin D (GSDMD) as a novel biomarker for early diagnosis and monitoring of type 2 diabetes mellitus.Methods: We conducted a comparative analysis of clinical indicators among newly diagnosed diabetes patients, those with prediabetes, and healthy controls, finding significantly elevated plasma GSDMD levels in the diabetes group (P < 0.05).Results: Correlation analyses revealed that GSDMD levels were positively associated with inflammatory markers and indicators of insulin resistance, while negatively correlating with HOMA-β (P < 0.001). Logistic regression analysis identified GSDMD, IL-6, and CRP as independent risk factors for type 2 diabetes (P < 0.001). The area under the ROC curve for plasma GSDMD was 0.988, indicating superior diagnostic capability compared to traditional markers like CRP (0.902) and IL-6 (0.857). With a threshold of 17.67 pg/mL, plasma GSDMD exhibited a sensitivity of 93.9% and specificity of 98.0%.Conclusion: These findings suggest that plasma GSDMD not only reflects early metabolic abnormalities associated with type 2 diabetes but also holds promise as a therapeutic target to mitigate inflammatory responses and improve insulin resistance. Further large-scale clinical studies are warranted to validate its diagnostic utility and enhance clinical applications.Keywords: biomarker discovery, gasdermin D, early diagnosis, type 2 diabetes mellitus, plasma biomarker, prediabeteshttps://www.dovepress.com/pyroptosis-mediator-gsdmd-in-plasma-a-promising-biomarker-for-early-di-peer-reviewed-fulltext-article-DMSObiomarker discoverygasdermin dearly diagnosistype 2 diabetes mellitusplasma biomarkerprediabetes
spellingShingle Huo L
Liu X
Ding N
Zhang H
Hou S
He J
Zhao B
Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes
Diabetes, Metabolic Syndrome and Obesity
biomarker discovery
gasdermin d
early diagnosis
type 2 diabetes mellitus
plasma biomarker
prediabetes
title Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes
title_full Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes
title_fullStr Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes
title_full_unstemmed Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes
title_short Pyroptosis-Mediator GSDMD in Plasma: A Promising Biomarker for Early Diagnosis of Type 2 Diabetes
title_sort pyroptosis mediator gsdmd in plasma a promising biomarker for early diagnosis of type 2 diabetes
topic biomarker discovery
gasdermin d
early diagnosis
type 2 diabetes mellitus
plasma biomarker
prediabetes
url https://www.dovepress.com/pyroptosis-mediator-gsdmd-in-plasma-a-promising-biomarker-for-early-di-peer-reviewed-fulltext-article-DMSO
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