Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target
Abstract Liver cancer is a serious malignancy worldwide, and long noncoding RNAs (lncRNAs) have been implicated in its prognosis.It remains unclear how lncRNAs related to endoplasmic reticulum stress (ERS) influence liver cancer prognosis. Here, we analyzed RNA and clinical data from the Cancer Geno...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
|
| Series: | BMC Medical Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12920-025-02142-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849699822323367936 |
|---|---|
| author | Xiao Du Ning Wei Anqi Wang Guoping Sun |
| author_facet | Xiao Du Ning Wei Anqi Wang Guoping Sun |
| author_sort | Xiao Du |
| collection | DOAJ |
| description | Abstract Liver cancer is a serious malignancy worldwide, and long noncoding RNAs (lncRNAs) have been implicated in its prognosis.It remains unclear how lncRNAs related to endoplasmic reticulum stress (ERS) influence liver cancer prognosis. Here, we analyzed RNA and clinical data from the Cancer Genome Atlas and sourced ERS-related genes from the Molecular Signatures Database. Co-expression analysis identified ERS-related lncRNAs, and Cox regression analysis as well as least absolute shrinkage and selection operator regression highlighted three lncRNAs for a prognostic model. Based on median risk scores, we classified patients into two risk groups. The high-risk group displayed poor prognosis, and this finding was validated in the test set. According to consistency clustering, the patients were assigned to two clusters, and tumor microenvironment scores were computed. Patients with a high mutation burden had worse outcomes. Furthermore, immune infiltration analysis indicated more immune cells and mutations in checkpoint molecules among high-risk individuals. Drug sensitivity varied between the risk groups. LINC01011 was selected for functional assays. Colony formation assay and CCK-8 assay revealed that silencing LINC01011 suppressed liver cancer cell proliferation. Transwell and scratch assays indicated that silencing LINC01011 inhibited liver cancer cell migration. Western blotting assay revealed that inhibiting LINC01011 induced apoptosis and simultaneously inhibited epithelial-mesenchymal transition. These findings confirm the validity of the prognostic model and indicate that LINC01011 could serve as a potential research target. |
| format | Article |
| id | doaj-art-bd3cecf58d2d4ac8ac276f2baeaf0a54 |
| institution | DOAJ |
| issn | 1755-8794 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj-art-bd3cecf58d2d4ac8ac276f2baeaf0a542025-08-20T03:18:28ZengBMCBMC Medical Genomics1755-87942025-04-0118111510.1186/s12920-025-02142-3Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic targetXiao Du0Ning Wei1Anqi Wang2Guoping Sun3Department of Oncology, the First Affiliated Hospital of Anhui Medical UniversityCheeloo College of Medicine, Shandong UniversityDepartment of Oncology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Oncology, the First Affiliated Hospital of Anhui Medical UniversityAbstract Liver cancer is a serious malignancy worldwide, and long noncoding RNAs (lncRNAs) have been implicated in its prognosis.It remains unclear how lncRNAs related to endoplasmic reticulum stress (ERS) influence liver cancer prognosis. Here, we analyzed RNA and clinical data from the Cancer Genome Atlas and sourced ERS-related genes from the Molecular Signatures Database. Co-expression analysis identified ERS-related lncRNAs, and Cox regression analysis as well as least absolute shrinkage and selection operator regression highlighted three lncRNAs for a prognostic model. Based on median risk scores, we classified patients into two risk groups. The high-risk group displayed poor prognosis, and this finding was validated in the test set. According to consistency clustering, the patients were assigned to two clusters, and tumor microenvironment scores were computed. Patients with a high mutation burden had worse outcomes. Furthermore, immune infiltration analysis indicated more immune cells and mutations in checkpoint molecules among high-risk individuals. Drug sensitivity varied between the risk groups. LINC01011 was selected for functional assays. Colony formation assay and CCK-8 assay revealed that silencing LINC01011 suppressed liver cancer cell proliferation. Transwell and scratch assays indicated that silencing LINC01011 inhibited liver cancer cell migration. Western blotting assay revealed that inhibiting LINC01011 induced apoptosis and simultaneously inhibited epithelial-mesenchymal transition. These findings confirm the validity of the prognostic model and indicate that LINC01011 could serve as a potential research target.https://doi.org/10.1186/s12920-025-02142-3Endoplasmic reticulum stressLncRNALiver cancerModel |
| spellingShingle | Xiao Du Ning Wei Anqi Wang Guoping Sun Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target BMC Medical Genomics Endoplasmic reticulum stress LncRNA Liver cancer Model |
| title | Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target |
| title_full | Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target |
| title_fullStr | Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target |
| title_full_unstemmed | Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target |
| title_short | Liver cancer-specific prognostic model developed using endoplasmic reticulum stress-related LncRNAs and LINC01011 as a potential therapeutic target |
| title_sort | liver cancer specific prognostic model developed using endoplasmic reticulum stress related lncrnas and linc01011 as a potential therapeutic target |
| topic | Endoplasmic reticulum stress LncRNA Liver cancer Model |
| url | https://doi.org/10.1186/s12920-025-02142-3 |
| work_keys_str_mv | AT xiaodu livercancerspecificprognosticmodeldevelopedusingendoplasmicreticulumstressrelatedlncrnasandlinc01011asapotentialtherapeutictarget AT ningwei livercancerspecificprognosticmodeldevelopedusingendoplasmicreticulumstressrelatedlncrnasandlinc01011asapotentialtherapeutictarget AT anqiwang livercancerspecificprognosticmodeldevelopedusingendoplasmicreticulumstressrelatedlncrnasandlinc01011asapotentialtherapeutictarget AT guopingsun livercancerspecificprognosticmodeldevelopedusingendoplasmicreticulumstressrelatedlncrnasandlinc01011asapotentialtherapeutictarget |