Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress

Background and aim: Approximately 80% of the population in developing countries is affected by gastric ulcers. Gastric ulcers are associated with pain, and there is an urgent need for drugs that can manage both conditions. Ricinodendron heudelotii (Baill.) Pierre ex. Heckel has long been used in man...

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Main Authors: Isaac Tabiri Henneh, Wisdom Ahlidja, Ernest Amponsah Asiamah, Denzel Opoku-Kwabi, Henry Mensah Korsah, Fynn Malcom, Stephen Mensah Arhin, Michael Buenor Adinortey, Francis Ackah Armah
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Language:English
Published: Elsevier 2025-07-01
Series:Journal of Traditional and Complementary Medicine
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Online Access:http://www.sciencedirect.com/science/article/pii/S2225411024000269
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author Isaac Tabiri Henneh
Wisdom Ahlidja
Ernest Amponsah Asiamah
Denzel Opoku-Kwabi
Henry Mensah Korsah
Fynn Malcom
Stephen Mensah Arhin
Michael Buenor Adinortey
Francis Ackah Armah
author_facet Isaac Tabiri Henneh
Wisdom Ahlidja
Ernest Amponsah Asiamah
Denzel Opoku-Kwabi
Henry Mensah Korsah
Fynn Malcom
Stephen Mensah Arhin
Michael Buenor Adinortey
Francis Ackah Armah
author_sort Isaac Tabiri Henneh
collection DOAJ
description Background and aim: Approximately 80% of the population in developing countries is affected by gastric ulcers. Gastric ulcers are associated with pain, and there is an urgent need for drugs that can manage both conditions. Ricinodendron heudelotii (Baill.) Pierre ex. Heckel has long been used in managing gastric ulcers and pain in African Traditional Medicine. In the present study, the antinociceptive and anti-ulcer activity of Ricinodendron heudelotii hydroethanolic stem bark extract (RHE) was evaluated. Experimental procedure: The antinociceptive effect of RHE was tested in ICR mice using the hot plate test, the glutamate-induced nociceptive test, and the formalin test. Gastric ulcers were induced in healthy male adult albino rats in acidified alcohol-induced ulcer (AAIU) and indomethacin-induced ulcer (IIU) models. After ulcer induction, the rats were treated with either RHE (30, 100, or 300 mg/kg, p.o.) or 10 mg/kg omeprazole. The stomachs of the sacrificed animals were removed for further analysis. Results and conclusion: RHE (30, 100, and 300 mg/kg) exhibited an anti-nociceptive effect in the hot plate, formalin and glutamate tests. The opioid receptor antagonist, naloxone, reversed the analgesic effect, indicating that the extract may be acting via the opioidergic pathway. But compared to the model group in both AAIU and IIU, oral administration of RHE decreased ulcer index, raised gastric pH levels, increased tissue levels of total antioxidant capacity, catalase, total thiols, and glutathione, and kept gastric histoarchitecture intact. RHE exhibited anti-nociceptive effects, possibly via the opioidergic pathway and anti-ulcer activity via reduction of oxidative stress.
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spelling doaj-art-bd37733665af44bb94f6ac1fa577d5b42025-08-20T02:35:19ZengElsevierJournal of Traditional and Complementary Medicine2225-41102025-07-0115433134210.1016/j.jtcme.2024.03.007Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stressIsaac Tabiri Henneh0Wisdom Ahlidja1Ernest Amponsah Asiamah2Denzel Opoku-Kwabi3Henry Mensah Korsah4Fynn Malcom5Stephen Mensah Arhin6Michael Buenor Adinortey7Francis Ackah Armah8Department of Pharmacotherapeutics and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana; Corresponding author.Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Chemistry, School of Physical Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana; Corresponding author.Background and aim: Approximately 80% of the population in developing countries is affected by gastric ulcers. Gastric ulcers are associated with pain, and there is an urgent need for drugs that can manage both conditions. Ricinodendron heudelotii (Baill.) Pierre ex. Heckel has long been used in managing gastric ulcers and pain in African Traditional Medicine. In the present study, the antinociceptive and anti-ulcer activity of Ricinodendron heudelotii hydroethanolic stem bark extract (RHE) was evaluated. Experimental procedure: The antinociceptive effect of RHE was tested in ICR mice using the hot plate test, the glutamate-induced nociceptive test, and the formalin test. Gastric ulcers were induced in healthy male adult albino rats in acidified alcohol-induced ulcer (AAIU) and indomethacin-induced ulcer (IIU) models. After ulcer induction, the rats were treated with either RHE (30, 100, or 300 mg/kg, p.o.) or 10 mg/kg omeprazole. The stomachs of the sacrificed animals were removed for further analysis. Results and conclusion: RHE (30, 100, and 300 mg/kg) exhibited an anti-nociceptive effect in the hot plate, formalin and glutamate tests. The opioid receptor antagonist, naloxone, reversed the analgesic effect, indicating that the extract may be acting via the opioidergic pathway. But compared to the model group in both AAIU and IIU, oral administration of RHE decreased ulcer index, raised gastric pH levels, increased tissue levels of total antioxidant capacity, catalase, total thiols, and glutathione, and kept gastric histoarchitecture intact. RHE exhibited anti-nociceptive effects, possibly via the opioidergic pathway and anti-ulcer activity via reduction of oxidative stress.http://www.sciencedirect.com/science/article/pii/S2225411024000269AntioxidantsAnti-ulcer agentsGastric ulcerOxidative stressRicinodendron heudelotii
spellingShingle Isaac Tabiri Henneh
Wisdom Ahlidja
Ernest Amponsah Asiamah
Denzel Opoku-Kwabi
Henry Mensah Korsah
Fynn Malcom
Stephen Mensah Arhin
Michael Buenor Adinortey
Francis Ackah Armah
Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress
Journal of Traditional and Complementary Medicine
Antioxidants
Anti-ulcer agents
Gastric ulcer
Oxidative stress
Ricinodendron heudelotii
title Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress
title_full Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress
title_fullStr Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress
title_full_unstemmed Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress
title_short Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress
title_sort antinociceptive and anti ulcerogenic effect of hydroethanolic stem bark extract of ricinodendron heudelotii baill pierre ex heckel euphorbiaceae involvement of the opioidergic pathway and attenuation of oxidative stress
topic Antioxidants
Anti-ulcer agents
Gastric ulcer
Oxidative stress
Ricinodendron heudelotii
url http://www.sciencedirect.com/science/article/pii/S2225411024000269
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