The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates
Oysters (Crassostrea gigas) have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their anti-diabetes effects and antioxidants. Oyster proteins were extracted from fresh oysters using...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302300006X |
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| author | Dongyang Zhu Zhen Yuan Di Wu Chao Wu Hesham R. El-Seedi Ming Du |
| author_facet | Dongyang Zhu Zhen Yuan Di Wu Chao Wu Hesham R. El-Seedi Ming Du |
| author_sort | Dongyang Zhu |
| collection | DOAJ |
| description | Oysters (Crassostrea gigas) have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their anti-diabetes effects and antioxidants. Oyster proteins were extracted from fresh oysters using phosphate-buffered saline and simulated gastrointestinal digestion was performed. The degree of hydrolysis (DH), structural characterization, molecular weight (Mw) distribution, free amino acid, anti-diabetic activity, and antioxidant activity were studied during in vitro simulated gastrointestinal digestion. The results showed that the α-glucosidase inhibitory activity, α-amylase inhibitory activity, DPPH radical scavenging activity, and ABTS radical scavenging activity of the oyster protein gastrointestinal digest were increased (P < 0.05) from 0 to 33.96 %, from 9.17 % to 44.22 %, from 9.01 µg trolox/mg protein to 18.48 µg trolox/mg protein, and from 21.44 µg trolox/mg protein to 56.21 µg trolox/mg protein, respectively. Additionally, the DH, β-turn structure, fluorescence intensity, free amino acid, and short peptide content (Mw < 1000 Da) increased in the simulated gastrointestinal digestion. These results indicate that the digestive hydrolysates obtained from oyster proteins could be used as natural anti-diabetic and antioxidant agents. |
| format | Article |
| id | doaj-art-bd2cba6a4274484fb0f946b88746a6ba |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-bd2cba6a4274484fb0f946b88746a6ba2025-02-03T05:40:25ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112516091617The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysatesDongyang Zhu0Zhen Yuan1Di Wu2Chao Wu3Hesham R. El-Seedi4Ming Du5School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaPharmacognosy Group, Department of Pharmaceutical Biosciences, Uppsala University, Biomedical Centre, Uppsala SE 751 24, Sweden; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China; Corresponding author.Oysters (Crassostrea gigas) have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their anti-diabetes effects and antioxidants. Oyster proteins were extracted from fresh oysters using phosphate-buffered saline and simulated gastrointestinal digestion was performed. The degree of hydrolysis (DH), structural characterization, molecular weight (Mw) distribution, free amino acid, anti-diabetic activity, and antioxidant activity were studied during in vitro simulated gastrointestinal digestion. The results showed that the α-glucosidase inhibitory activity, α-amylase inhibitory activity, DPPH radical scavenging activity, and ABTS radical scavenging activity of the oyster protein gastrointestinal digest were increased (P < 0.05) from 0 to 33.96 %, from 9.17 % to 44.22 %, from 9.01 µg trolox/mg protein to 18.48 µg trolox/mg protein, and from 21.44 µg trolox/mg protein to 56.21 µg trolox/mg protein, respectively. Additionally, the DH, β-turn structure, fluorescence intensity, free amino acid, and short peptide content (Mw < 1000 Da) increased in the simulated gastrointestinal digestion. These results indicate that the digestive hydrolysates obtained from oyster proteins could be used as natural anti-diabetic and antioxidant agents.http://www.sciencedirect.com/science/article/pii/S221345302300006XOyster proteinsSimulated gastrointestinal digestionAnti-diabetic activityAntioxidant activityFree amino acid |
| spellingShingle | Dongyang Zhu Zhen Yuan Di Wu Chao Wu Hesham R. El-Seedi Ming Du The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates Food Science and Human Wellness Oyster proteins Simulated gastrointestinal digestion Anti-diabetic activity Antioxidant activity Free amino acid |
| title | The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates |
| title_full | The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates |
| title_fullStr | The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates |
| title_full_unstemmed | The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates |
| title_short | The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates |
| title_sort | dual function of bioactive peptides derived from oyster crassostrea gigas proteins hydrolysates |
| topic | Oyster proteins Simulated gastrointestinal digestion Anti-diabetic activity Antioxidant activity Free amino acid |
| url | http://www.sciencedirect.com/science/article/pii/S221345302300006X |
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