High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices
Staphylococcal enterotoxins (SEs) serve as the primary cause of staphylococcal food poisoning and other foodborne intoxications. Among them, staphylococcal enterotoxin C (SEC) has the highest prevalence in dairy products, leading to multiple outbreaks all around the world. Thus, it is of great signi...
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Elsevier
2024-12-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024167060 |
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| author | Chunmei Zhang Yuanjie Sun Xiyang Zhang Yongming Li Zhijia Liu Shuya Yang Dongbo Jiang Jing Wang Boquan Jin Yun Zhang Kun Yang |
| author_facet | Chunmei Zhang Yuanjie Sun Xiyang Zhang Yongming Li Zhijia Liu Shuya Yang Dongbo Jiang Jing Wang Boquan Jin Yun Zhang Kun Yang |
| author_sort | Chunmei Zhang |
| collection | DOAJ |
| description | Staphylococcal enterotoxins (SEs) serve as the primary cause of staphylococcal food poisoning and other foodborne intoxications. Among them, staphylococcal enterotoxin C (SEC) has the highest prevalence in dairy products, leading to multiple outbreaks all around the world. Thus, it is of great significance to develop a highly sensitive, highly specific and easy to operate chemiluminescent sandwich enzyme immunoassay (CLEIA) for detecting staphylococcal enterotoxin C (SEC1). We selected two pairs of anti-SEC1 monoclonal antibodies (mAbs) (SEC1-G8 and SEC1-C4), and a chemiluminescent sandwich enzyme immunoassay (CLEIA) was constructed. This approach can detect SEC1 within a concentration spectrum of 3.2–4000 pg/mL, with the detection limit being 2.1 pg/mL. At three concentrations (3.2, 20, and 400 pg/mL), both the intra- and inter-assay coefficient variations were coming in at 6.31 % and 11.2 % respectively. No cross-reaction was noticed in the SEA, SEB, and SED tests. SEC1 was successfully detected by employing the CLEIA method in spiked matrices and commercial samples, and the average recovery rate ranges from 81.6 % to 108.1 %. Therefore, the highly sensitive, SEC1- specific, and easy-to-operate CLEIA could be a useful tool in the near future for quantifying SEC1 in public health and food safety. |
| format | Article |
| id | doaj-art-bd209c86da494eaebf8a36d6e5f39c92 |
| institution | OA Journals |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-bd209c86da494eaebf8a36d6e5f39c922025-08-20T01:59:31ZengElsevierHeliyon2405-84402024-12-011023e4067510.1016/j.heliyon.2024.e40675High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matricesChunmei Zhang0Yuanjie Sun1Xiyang Zhang2Yongming Li3Zhijia Liu4Shuya Yang5Dongbo Jiang6Jing Wang7Boquan Jin8Yun Zhang9Kun Yang10Department of Immunology, The Fourth Military Medical University, Xi'an, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, ChinaLaboratory Department, Affiliated Hospital of Army Medical University NCO School, Shijiazhuang, ChinaDepartment of Urology, The Eighth Medical Center of Chinese People's Liberation Army General Hospital, Beijing, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, ChinaDepartment of Immunology, The Fourth Military Medical University, Xi'an, China; Corresponding author.Department of Immunology, The Fourth Military Medical University, Xi'an, China; Corresponding author. Department of Immunology, The Fourth Military Medical University, 169 Changle West Road, Xincheng District, Xi'an, 710032, China.Staphylococcal enterotoxins (SEs) serve as the primary cause of staphylococcal food poisoning and other foodborne intoxications. Among them, staphylococcal enterotoxin C (SEC) has the highest prevalence in dairy products, leading to multiple outbreaks all around the world. Thus, it is of great significance to develop a highly sensitive, highly specific and easy to operate chemiluminescent sandwich enzyme immunoassay (CLEIA) for detecting staphylococcal enterotoxin C (SEC1). We selected two pairs of anti-SEC1 monoclonal antibodies (mAbs) (SEC1-G8 and SEC1-C4), and a chemiluminescent sandwich enzyme immunoassay (CLEIA) was constructed. This approach can detect SEC1 within a concentration spectrum of 3.2–4000 pg/mL, with the detection limit being 2.1 pg/mL. At three concentrations (3.2, 20, and 400 pg/mL), both the intra- and inter-assay coefficient variations were coming in at 6.31 % and 11.2 % respectively. No cross-reaction was noticed in the SEA, SEB, and SED tests. SEC1 was successfully detected by employing the CLEIA method in spiked matrices and commercial samples, and the average recovery rate ranges from 81.6 % to 108.1 %. Therefore, the highly sensitive, SEC1- specific, and easy-to-operate CLEIA could be a useful tool in the near future for quantifying SEC1 in public health and food safety.http://www.sciencedirect.com/science/article/pii/S2405844024167060Staphylococcal enterotoxin C1Chemiluminescence enzyme immunoassaySensitivityStaphylococcal food poisoningApplicationFood safety |
| spellingShingle | Chunmei Zhang Yuanjie Sun Xiyang Zhang Yongming Li Zhijia Liu Shuya Yang Dongbo Jiang Jing Wang Boquan Jin Yun Zhang Kun Yang High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices Heliyon Staphylococcal enterotoxin C1 Chemiluminescence enzyme immunoassay Sensitivity Staphylococcal food poisoning Application Food safety |
| title | High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices |
| title_full | High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices |
| title_fullStr | High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices |
| title_full_unstemmed | High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices |
| title_short | High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices |
| title_sort | high sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin c1 and its application in multi matrices |
| topic | Staphylococcal enterotoxin C1 Chemiluminescence enzyme immunoassay Sensitivity Staphylococcal food poisoning Application Food safety |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024167060 |
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