RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells
Abstract Background Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammatio...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-12-01
|
| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-022-00787-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850136668465528832 |
|---|---|
| author | Weilei Yao Tongxin Wang Lu Huang Zhengxi Bao Shu Wen Feiruo Huang |
| author_facet | Weilei Yao Tongxin Wang Lu Huang Zhengxi Bao Shu Wen Feiruo Huang |
| author_sort | Weilei Yao |
| collection | DOAJ |
| description | Abstract Background Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine model. One hundred sixty 21-day-old castrated weaned pigs (Duroc × Landrace × Yorkshire, average initial body weight was 7.05 ± 0.28 kg, equal numbers of castrated males and females) were allotted to four groups and fed with a basal diet or a basal diet containing 200, 400, or 600 mg embelin/kg for 28 d. The growth performance, intestinal inflammatory cytokines, morphology of jejunum and ileum, tight junctions in the intestinal mucosa of piglets were tested. IPEC-1 cells with overexpression of P300/CBP associating factor (PCAF) were treated with embelin, the activity of PCAF and acetylation of nuclear factor-κB (NF-κB) were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in vitro. Results The results showed that embelin decreased (P < 0.05) serum D-lactate and diamine oxidase (DAO) levels, and enhanced the expression of ZO-1, occludin and claudin-1 protein in jejunum and ileum. Moreover, the expression levels of critical inflammation molecules (interleukin-1β, interleukin-6, tumor necrosis factor-α, and NF-κB) were down-regulated (P < 0.05) by embelin in jejunal and ileal mucosa. Meanwhile, the activity of PCAF were down-regulated (P < 0.05) by embelin. Importantly, transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities; embelin treatment downregulated (P < 0.05) the acetylation and activities of NF-κB by 31.7%–74.6% in IPEC-1 cells with overexpression of PCAF. Conclusions These results suggested that embelin ameliorates intestinal inflammation in weaned pigs, which might be mediated by suppressing the PCAF/NF-κB signaling pathway. |
| format | Article |
| id | doaj-art-bd1dd1d5767d479fb1b147258cdf8925 |
| institution | OA Journals |
| issn | 2049-1891 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj-art-bd1dd1d5767d479fb1b147258cdf89252025-08-20T02:31:03ZengBMCJournal of Animal Science and Biotechnology2049-18912022-12-0113111010.1186/s40104-022-00787-zRETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cellsWeilei Yao0Tongxin Wang1Lu Huang2Zhengxi Bao3Shu Wen4Feiruo Huang5Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityAbstract Background Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine model. One hundred sixty 21-day-old castrated weaned pigs (Duroc × Landrace × Yorkshire, average initial body weight was 7.05 ± 0.28 kg, equal numbers of castrated males and females) were allotted to four groups and fed with a basal diet or a basal diet containing 200, 400, or 600 mg embelin/kg for 28 d. The growth performance, intestinal inflammatory cytokines, morphology of jejunum and ileum, tight junctions in the intestinal mucosa of piglets were tested. IPEC-1 cells with overexpression of P300/CBP associating factor (PCAF) were treated with embelin, the activity of PCAF and acetylation of nuclear factor-κB (NF-κB) were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in vitro. Results The results showed that embelin decreased (P < 0.05) serum D-lactate and diamine oxidase (DAO) levels, and enhanced the expression of ZO-1, occludin and claudin-1 protein in jejunum and ileum. Moreover, the expression levels of critical inflammation molecules (interleukin-1β, interleukin-6, tumor necrosis factor-α, and NF-κB) were down-regulated (P < 0.05) by embelin in jejunal and ileal mucosa. Meanwhile, the activity of PCAF were down-regulated (P < 0.05) by embelin. Importantly, transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities; embelin treatment downregulated (P < 0.05) the acetylation and activities of NF-κB by 31.7%–74.6% in IPEC-1 cells with overexpression of PCAF. Conclusions These results suggested that embelin ameliorates intestinal inflammation in weaned pigs, which might be mediated by suppressing the PCAF/NF-κB signaling pathway.https://doi.org/10.1186/s40104-022-00787-zAcetylation, InflammationIntestinal barrierPCAFWeaned pig |
| spellingShingle | Weilei Yao Tongxin Wang Lu Huang Zhengxi Bao Shu Wen Feiruo Huang RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells Journal of Animal Science and Biotechnology Acetylation, Inflammation Intestinal barrier PCAF Weaned pig |
| title | RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
| title_full | RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
| title_fullStr | RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
| title_full_unstemmed | RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
| title_short | RETRACTED ARTICLE: Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
| title_sort | retracted article embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via pcaf nf κb signaling pathway in intestinal epithelial cells |
| topic | Acetylation, Inflammation Intestinal barrier PCAF Weaned pig |
| url | https://doi.org/10.1186/s40104-022-00787-z |
| work_keys_str_mv | AT weileiyao retractedarticleembelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT tongxinwang retractedarticleembelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT luhuang retractedarticleembelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT zhengxibao retractedarticleembelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT shuwen retractedarticleembelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT feiruohuang retractedarticleembelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells |