Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells
Objective Hair morphogenesis is tightly related to hair follicle stem cells (HFSCs) proliferation and hair follicle (HF) development. Yangtze River Delta white goats (YRDWG) HFSCs are important for producing superior-quality brush hair (SQBH). Nonetheless, the known regulatory mechanisms are not suf...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Asian-Australasian Association of Animal Production Societies
2025-06-01
|
| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0816.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850040515242754048 |
|---|---|
| author | Jian Wang Xi Wu Liuming Zhang Xiaomei Sun Wei Sun Kunzhe Dong Yongjun Li |
| author_facet | Jian Wang Xi Wu Liuming Zhang Xiaomei Sun Wei Sun Kunzhe Dong Yongjun Li |
| author_sort | Jian Wang |
| collection | DOAJ |
| description | Objective Hair morphogenesis is tightly related to hair follicle stem cells (HFSCs) proliferation and hair follicle (HF) development. Yangtze River Delta white goats (YRDWG) HFSCs are important for producing superior-quality brush hair (SQBH). Nonetheless, the known regulatory mechanisms are not sufficient to explain YRDWG gHFSCs growth, HF development, and SQBH formation. Methods To deeply investigate the interaction networks and mechanisms of circCOL1A1 in the HF development and SQBH formation of YRDWG in detail, we applied whole-transcriptome sequencing and bioinformatics analysis of circCOL1A1-knockdown and circCOL1A1-overexpressing HFSCs from YRDWG. STRING and other databases were used to construct multiple interaction networks. Differentially expressed (DE) genes, DE-miRNAs, and DE-circRNAs were further confirmed via real-time quantitative polymerase chain reaction and Sanger sequencing. Results A total of 87 genes, 96 miRNAs, and 135 circRNAs were DE between circCOL1A1-knockdown and circCOL1A1-overexpressing gHFSCs. Functional enrichment, gene ontology annotation and Kyoto encyclopedia of genes and genomes analyses identified marked enrichment of these DE- genes, DE-miRNAs, and DE-circRNAs in the MAPK, PI3K/Akt, and focal adhesion signaling pathways, which are closely associated with gHFSCs growth and HF development. In addition, through interaction network construction, four important regulatory axes were obtained, namely, the chi-circCOL1A1-miR-149-5p-CMTM3-AR, chi-circACTN1- miR-671-5p-MAPK3/COL13A1, chi-circITGA6-miR-18a-5p-FGF1/MAP3K1 and chi-circ COBLL1-miR-30a-5p/miR-128-3p-ITGA6/MAPK14/FGF14 axes. Conclusion These novel findings provide a valuable and comprehensive basis for investigating the complex mechanism by which circRNAs participate in and regulate HF development and SQBH formation in YRDWG. |
| format | Article |
| id | doaj-art-bd192f2f1cbc4809879d19c1c8de3776 |
| institution | DOAJ |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-bd192f2f1cbc4809879d19c1c8de37762025-08-20T02:56:05ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-06-013861116113910.5713/ab.24.081625411Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cellsJian Wang0Xi Wu1Liuming Zhang2Xiaomei Sun3Wei Sun4Kunzhe Dong5Yongjun Li6 Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China Immunology Center of Georgia, Medical College of Georgia, Augusta University, Augusta, Georgia, USA Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaObjective Hair morphogenesis is tightly related to hair follicle stem cells (HFSCs) proliferation and hair follicle (HF) development. Yangtze River Delta white goats (YRDWG) HFSCs are important for producing superior-quality brush hair (SQBH). Nonetheless, the known regulatory mechanisms are not sufficient to explain YRDWG gHFSCs growth, HF development, and SQBH formation. Methods To deeply investigate the interaction networks and mechanisms of circCOL1A1 in the HF development and SQBH formation of YRDWG in detail, we applied whole-transcriptome sequencing and bioinformatics analysis of circCOL1A1-knockdown and circCOL1A1-overexpressing HFSCs from YRDWG. STRING and other databases were used to construct multiple interaction networks. Differentially expressed (DE) genes, DE-miRNAs, and DE-circRNAs were further confirmed via real-time quantitative polymerase chain reaction and Sanger sequencing. Results A total of 87 genes, 96 miRNAs, and 135 circRNAs were DE between circCOL1A1-knockdown and circCOL1A1-overexpressing gHFSCs. Functional enrichment, gene ontology annotation and Kyoto encyclopedia of genes and genomes analyses identified marked enrichment of these DE- genes, DE-miRNAs, and DE-circRNAs in the MAPK, PI3K/Akt, and focal adhesion signaling pathways, which are closely associated with gHFSCs growth and HF development. In addition, through interaction network construction, four important regulatory axes were obtained, namely, the chi-circCOL1A1-miR-149-5p-CMTM3-AR, chi-circACTN1- miR-671-5p-MAPK3/COL13A1, chi-circITGA6-miR-18a-5p-FGF1/MAP3K1 and chi-circ COBLL1-miR-30a-5p/miR-128-3p-ITGA6/MAPK14/FGF14 axes. Conclusion These novel findings provide a valuable and comprehensive basis for investigating the complex mechanism by which circRNAs participate in and regulate HF development and SQBH formation in YRDWG.http://www.animbiosci.org/upload/pdf/ab-24-0816.pdfcirccol1a1differentially expressedghfscsregulatory axiswhole-transcriptome sequencing |
| spellingShingle | Jian Wang Xi Wu Liuming Zhang Xiaomei Sun Wei Sun Kunzhe Dong Yongjun Li Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells Animal Bioscience circcol1a1 differentially expressed ghfscs regulatory axis whole-transcriptome sequencing |
| title | Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells |
| title_full | Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells |
| title_fullStr | Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells |
| title_full_unstemmed | Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells |
| title_short | Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells |
| title_sort | integrative and comparative analysis of whole transcriptome sequencing in circcol1a1 knockdown and circcol1a1 overexpressing goat hair follicle stem cells |
| topic | circcol1a1 differentially expressed ghfscs regulatory axis whole-transcriptome sequencing |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0816.pdf |
| work_keys_str_mv | AT jianwang integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells AT xiwu integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells AT liumingzhang integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells AT xiaomeisun integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells AT weisun integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells AT kunzhedong integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells AT yongjunli integrativeandcomparativeanalysisofwholetranscriptomesequencingincirccol1a1knockdownandcirccol1a1overexpressinggoathairfolliclestemcells |