Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception
Background Prolidase is a manganese (Mn)-dependent cytosolic exopeptidase that degrades imidodipeptides with C-terminal proline or hydroxyproline. Prolidase recycling from imidodipeptides plays a critical role in collagen resynthesis and extracellular matrix (ECM) remodelling. Following an increase...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2346228 |
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| author | Kadriye Erdoğan Emine Utlu Özen İnci Kahyaoğlu Salim Neselioglu Özcan Erel Serra Akar Özhan Özdemir Cihangir Mutlu Ercan Yaprak Engin Üstün |
| author_facet | Kadriye Erdoğan Emine Utlu Özen İnci Kahyaoğlu Salim Neselioglu Özcan Erel Serra Akar Özhan Özdemir Cihangir Mutlu Ercan Yaprak Engin Üstün |
| author_sort | Kadriye Erdoğan |
| collection | DOAJ |
| description | Background Prolidase is a manganese (Mn)-dependent cytosolic exopeptidase that degrades imidodipeptides with C-terminal proline or hydroxyproline. Prolidase recycling from imidodipeptides plays a critical role in collagen resynthesis and extracellular matrix (ECM) remodelling. Following an increase in gonadotropins, ovarian and follicular collagen undergo substantial degradation. Abnormal ovarian ECM composition is associated with polycystic ovary syndrome (PCOS). This study aimed to examine prolidase activity in the serum and follicular fluid (FF) of women undergoing in vitro fertilisation/intracytoplasmic sperm injection (IVF/ICSI) treatment, comparing those with PCOS to those with normal ovarian function.Methods This prospective study enrolled 50 participants, of whom 44 were included. PCOS diagnosis followed the Rotterdam consensus criteria, with 20 patients constituting the study group. The control group comprised 24 individuals with mild-to-moderate male infertility. Prolidase enzyme activity in serum and FF was measured using the Chinard reagent via spectrophotometric analysis and compared between the groups.Results Serum and FF prolidase levels were significantly lower in patients with PCOS (p < 0.05). A direct correlation was observed between serum and FF prolidase levels (p < 0.05). Although blastocyst quality scoring (BQS) significantly decreased in PCOS patients, no statistical difference was observed in the clinical pregnancy rate between the groups (p < 0.05) (p > 0.05). A negative correlation existed between serum prolidase levels and total antral follicle (AF) count (p < 0.05). Conversely, both serum and FF prolidase levels positively correlated with BQS (r = 0.574)(p < 0.05) (r = 0.650)(p < 0.05).Conclusions Patients with PCOS showed lower serum and FF prolidase levels, indicating abnormal degradation of ovarian and follicular collagen, potentially causing anovulation. |
| format | Article |
| id | doaj-art-bd00b7c323464e6f9dbaa984e62ae8fa |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-bd00b7c323464e6f9dbaa984e62ae8fa2025-01-09T12:13:18ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2346228Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conceptionKadriye Erdoğan0Emine Utlu Özen1İnci Kahyaoğlu2Salim Neselioglu3Özcan Erel4Serra Akar5Özhan Özdemir6Cihangir Mutlu Ercan7Yaprak Engin Üstün8Department of Obstetrics and Gynecology, Health Sciences University, Etlik Zübeyde Hanım Women’s Health Training and Research Hospital, Ankara, TurkeyDepartment of Embryology, Health Sciences University, Etlik Zübeyde Hanım Women’s Health Training and Research Hospital, Ankara, TurkeyDepartment of Obstetrics and Gynecology, Health Sciences University, Etlik Zübeyde Hanım Women’s Health Training and Research Hospital, Ankara, TurkeyDepartment of Biochemistry, University of Health Sciences, Ankara City Hospital, Ankara, TurkeyDepartment of Biochemistry, University of Health Sciences, Ankara City Hospital, Ankara, TurkeyDepartment of Obstetrics and Gynaecology, University of Health Sciences, Ankara City Hospital, Ankara, TurkeyDepartment of Obstetrics and Gynecology, Gülhane Training and Research Hospitals, Ankara, TurkeyDepartment of Obstetrics and Gynecology, Gülhane Training and Research Hospitals, Ankara, TurkeyDepartment of Obstetrics and Gynecology, Health Sciences University, Etlik Zübeyde Hanım Women’s Health Training and Research Hospital, Ankara, TurkeyBackground Prolidase is a manganese (Mn)-dependent cytosolic exopeptidase that degrades imidodipeptides with C-terminal proline or hydroxyproline. Prolidase recycling from imidodipeptides plays a critical role in collagen resynthesis and extracellular matrix (ECM) remodelling. Following an increase in gonadotropins, ovarian and follicular collagen undergo substantial degradation. Abnormal ovarian ECM composition is associated with polycystic ovary syndrome (PCOS). This study aimed to examine prolidase activity in the serum and follicular fluid (FF) of women undergoing in vitro fertilisation/intracytoplasmic sperm injection (IVF/ICSI) treatment, comparing those with PCOS to those with normal ovarian function.Methods This prospective study enrolled 50 participants, of whom 44 were included. PCOS diagnosis followed the Rotterdam consensus criteria, with 20 patients constituting the study group. The control group comprised 24 individuals with mild-to-moderate male infertility. Prolidase enzyme activity in serum and FF was measured using the Chinard reagent via spectrophotometric analysis and compared between the groups.Results Serum and FF prolidase levels were significantly lower in patients with PCOS (p < 0.05). A direct correlation was observed between serum and FF prolidase levels (p < 0.05). Although blastocyst quality scoring (BQS) significantly decreased in PCOS patients, no statistical difference was observed in the clinical pregnancy rate between the groups (p < 0.05) (p > 0.05). A negative correlation existed between serum prolidase levels and total antral follicle (AF) count (p < 0.05). Conversely, both serum and FF prolidase levels positively correlated with BQS (r = 0.574)(p < 0.05) (r = 0.650)(p < 0.05).Conclusions Patients with PCOS showed lower serum and FF prolidase levels, indicating abnormal degradation of ovarian and follicular collagen, potentially causing anovulation.https://www.tandfonline.com/doi/10.1080/01443615.2024.2346228Assisted conceptionpolycystic ovary syndromeprolidase activity |
| spellingShingle | Kadriye Erdoğan Emine Utlu Özen İnci Kahyaoğlu Salim Neselioglu Özcan Erel Serra Akar Özhan Özdemir Cihangir Mutlu Ercan Yaprak Engin Üstün Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception Journal of Obstetrics and Gynaecology Assisted conception polycystic ovary syndrome prolidase activity |
| title | Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception |
| title_full | Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception |
| title_fullStr | Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception |
| title_full_unstemmed | Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception |
| title_short | Prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception |
| title_sort | prolidase activity in women with polycystic ovarian syndrome undergoing assisted conception |
| topic | Assisted conception polycystic ovary syndrome prolidase activity |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2346228 |
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