Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz
Introduction. Efavirenz is an antihuman immunodeficiency virus (HIV) drug metabolized by cytochrome P450 2B6 (CYP2B6) enzyme. Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. Polymorphisms of this gene play a crucial role in the metabolism of drugs such as Efavirenz. Th...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2022/5754776 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849685001838264320 |
|---|---|
| author | Parham Mardi Bahareh Tavakoli-Far Samira Sheibani Nia Roshanak Jazayeri Massoud Houshmand |
| author_facet | Parham Mardi Bahareh Tavakoli-Far Samira Sheibani Nia Roshanak Jazayeri Massoud Houshmand |
| author_sort | Parham Mardi |
| collection | DOAJ |
| description | Introduction. Efavirenz is an antihuman immunodeficiency virus (HIV) drug metabolized by cytochrome P450 2B6 (CYP2B6) enzyme. Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. Polymorphisms of this gene play a crucial role in the metabolism of drugs such as Efavirenz. This study aims to evaluate the frequency of three clinically significant CYP2B6 polymorphisms (CYP2B6∗6 (516G > T), CYP2B6∗4 (785A > G), and CYP2B6∗5 (1459C > T)) in three major Iranian ethnicities. Methods. One hundred forty-seven participants from three main Iranian ethnicities were included in this study. After DNA extraction, CYP2B6∗6 (516G > T), CYP2B6∗4 (785A > G), and CYP2B6∗5 (1459C > T) were genotyped using tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR). Results. The frequency of the mutated allele in the Iranian population for CYP2B6∗6 (516G > T) was 41.50 (95% CI: 35.81, 47.36), which was significantly lower than in Kurds (59.62, 95% CI: 45.10, 72.99). Similarly, Kurds had a higher frequency of mutated allele of CYP2B6∗5 (1459C > T) (46.15%, 95% CI: 32.23, 60.53) than in Iranians (24.49%, 95% CI: 19.68, 29.82). The frequency of A and G alleles of CYP2B6∗4 (785A > G) was 62.59% (95% CI: 56.78, 68.13) and 37.41 (95% CI: 31.87, 43.22), respectively. Conclusion. Kurds are at higher risk of adverse drug reactions (ADRs) and insufficient anti-HIV response compared to other Iranians. |
| format | Article |
| id | doaj-art-bcfb6c146c8642bc9cbde8dedd5886a0 |
| institution | DOAJ |
| issn | 1469-5073 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj-art-bcfb6c146c8642bc9cbde8dedd5886a02025-08-20T03:23:18ZengWileyGenetics Research1469-50732022-01-01202210.1155/2022/5754776Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to EfavirenzParham Mardi0Bahareh Tavakoli-Far1Samira Sheibani Nia2Roshanak Jazayeri3Massoud Houshmand4Student Research CommitteeDietary Supplements and Probiotic Research CenterDepartment of Medical GeneticsNon-communicable Diseases Research CenterDepartment of Medical GeneticsIntroduction. Efavirenz is an antihuman immunodeficiency virus (HIV) drug metabolized by cytochrome P450 2B6 (CYP2B6) enzyme. Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. Polymorphisms of this gene play a crucial role in the metabolism of drugs such as Efavirenz. This study aims to evaluate the frequency of three clinically significant CYP2B6 polymorphisms (CYP2B6∗6 (516G > T), CYP2B6∗4 (785A > G), and CYP2B6∗5 (1459C > T)) in three major Iranian ethnicities. Methods. One hundred forty-seven participants from three main Iranian ethnicities were included in this study. After DNA extraction, CYP2B6∗6 (516G > T), CYP2B6∗4 (785A > G), and CYP2B6∗5 (1459C > T) were genotyped using tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR). Results. The frequency of the mutated allele in the Iranian population for CYP2B6∗6 (516G > T) was 41.50 (95% CI: 35.81, 47.36), which was significantly lower than in Kurds (59.62, 95% CI: 45.10, 72.99). Similarly, Kurds had a higher frequency of mutated allele of CYP2B6∗5 (1459C > T) (46.15%, 95% CI: 32.23, 60.53) than in Iranians (24.49%, 95% CI: 19.68, 29.82). The frequency of A and G alleles of CYP2B6∗4 (785A > G) was 62.59% (95% CI: 56.78, 68.13) and 37.41 (95% CI: 31.87, 43.22), respectively. Conclusion. Kurds are at higher risk of adverse drug reactions (ADRs) and insufficient anti-HIV response compared to other Iranians.http://dx.doi.org/10.1155/2022/5754776 |
| spellingShingle | Parham Mardi Bahareh Tavakoli-Far Samira Sheibani Nia Roshanak Jazayeri Massoud Houshmand Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz Genetics Research |
| title | Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz |
| title_full | Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz |
| title_fullStr | Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz |
| title_full_unstemmed | Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz |
| title_short | Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz |
| title_sort | frequency of cyp2b6 alleles in major iranian ethnicities affecting response to efavirenz |
| url | http://dx.doi.org/10.1155/2022/5754776 |
| work_keys_str_mv | AT parhammardi frequencyofcyp2b6allelesinmajoriranianethnicitiesaffectingresponsetoefavirenz AT baharehtavakolifar frequencyofcyp2b6allelesinmajoriranianethnicitiesaffectingresponsetoefavirenz AT samirasheibaninia frequencyofcyp2b6allelesinmajoriranianethnicitiesaffectingresponsetoefavirenz AT roshanakjazayeri frequencyofcyp2b6allelesinmajoriranianethnicitiesaffectingresponsetoefavirenz AT massoudhoushmand frequencyofcyp2b6allelesinmajoriranianethnicitiesaffectingresponsetoefavirenz |