Research Progress on Chitinase and Chitosanase with Transglycosylation Activity

N−acetyl chitooligosaccharides and chitooligosaccharides with high degree of polymerization are highly valued for their superior bioactivities. However, traditional preparation methods face various limitations, such as uncontrollable processes and challenges inseparation and purification. Recent stu...

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Main Authors: Yingying WEI, Minjuan GUI, Huihui SUN, Ling ZHAO, Rong CAO
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-07-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070134
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author Yingying WEI
Minjuan GUI
Huihui SUN
Ling ZHAO
Rong CAO
author_facet Yingying WEI
Minjuan GUI
Huihui SUN
Ling ZHAO
Rong CAO
author_sort Yingying WEI
collection DOAJ
description N−acetyl chitooligosaccharides and chitooligosaccharides with high degree of polymerization are highly valued for their superior bioactivities. However, traditional preparation methods face various limitations, such as uncontrollable processes and challenges inseparation and purification. Recent studies have discovered that certain chitinases and chitosanases possess both hydrolysis and transglycosylation (TG) activities. The TG reaction offers advantages in producing oligosaccharides with high-degree polymerization, including easy accessibility to substrates and low production costs. This paper reviews recent advances in chitinases and chitosanases with TG activity, analyzes the key amino acid residues affecting TG activity in chitinases, and discusses the impact of molecular modifications of these key residues on TG activity. The goal is to provide insights for further research on chitinases and chitosanases with TG activity and their subsequent development and utilization, thereby promoting the green production and industrialization of oligosaccharides with high-degree polymerization.
format Article
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publishDate 2025-07-01
publisher The editorial department of Science and Technology of Food Industry
record_format Article
series Shipin gongye ke-ji
spelling doaj-art-bcf56cc0d4894c2bb5f4312c35baaa7a2025-08-20T03:23:56ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-07-01461340441210.13386/j.issn1002-0306.20240701342024070134-13Research Progress on Chitinase and Chitosanase with Transglycosylation ActivityYingying WEI0Minjuan GUI1Huihui SUN2Ling ZHAO3Rong CAO4College of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaN−acetyl chitooligosaccharides and chitooligosaccharides with high degree of polymerization are highly valued for their superior bioactivities. However, traditional preparation methods face various limitations, such as uncontrollable processes and challenges inseparation and purification. Recent studies have discovered that certain chitinases and chitosanases possess both hydrolysis and transglycosylation (TG) activities. The TG reaction offers advantages in producing oligosaccharides with high-degree polymerization, including easy accessibility to substrates and low production costs. This paper reviews recent advances in chitinases and chitosanases with TG activity, analyzes the key amino acid residues affecting TG activity in chitinases, and discusses the impact of molecular modifications of these key residues on TG activity. The goal is to provide insights for further research on chitinases and chitosanases with TG activity and their subsequent development and utilization, thereby promoting the green production and industrialization of oligosaccharides with high-degree polymerization.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070134transglycosylation activitychitinasechitosanasehigh deep of polymerizationmolecular modification
spellingShingle Yingying WEI
Minjuan GUI
Huihui SUN
Ling ZHAO
Rong CAO
Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
Shipin gongye ke-ji
transglycosylation activity
chitinase
chitosanase
high deep of polymerization
molecular modification
title Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
title_full Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
title_fullStr Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
title_full_unstemmed Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
title_short Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
title_sort research progress on chitinase and chitosanase with transglycosylation activity
topic transglycosylation activity
chitinase
chitosanase
high deep of polymerization
molecular modification
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070134
work_keys_str_mv AT yingyingwei researchprogressonchitinaseandchitosanasewithtransglycosylationactivity
AT minjuangui researchprogressonchitinaseandchitosanasewithtransglycosylationactivity
AT huihuisun researchprogressonchitinaseandchitosanasewithtransglycosylationactivity
AT lingzhao researchprogressonchitinaseandchitosanasewithtransglycosylationactivity
AT rongcao researchprogressonchitinaseandchitosanasewithtransglycosylationactivity